Plasmids 101: Modular Cloning
Type
Blog Post
...Gate Assembly with NEB’s DAD
Interview with Nicola Patron on MoClo
Plasmids 101: Golden Gate Cloning
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We narrowed to 682 results for: RON
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CRISPR in the Clinic
TypeBlog Post...editing to treat sickle cell disease-A brief update. Front Med (Lausanne). 2023 Jan 9;9:1065377. doi: 10.3389... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...some older split-GFP constructs or certain cell environments). Also consider that a protein fusion may disrupt... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post... S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann ... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post... contain a short summary of the topic and a chronological list of highly cited papers that contain your... -
Viral Vectors 101: Virus Safety
TypeBlog Post...cultures do, and tired researchers are accident-prone researchers. Note that safety policies will vary... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...intracellular injections into the cytoplasm or pronucleus of the zygote which is both technically challenging... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog Post...control mice will have been raised in the same environment and will harbor similar epigenetic modifications... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...organisation like Building with Biology. Local environmental groups, charities and NGOs are often looking... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...tags (e.g. FLAG and STREP) and auxin-inducible degrons (AID). Further, the Yamamoto PITCh system plasmids... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post... For plasmids with highly repetitive sequences (prone to recombination in bacteria) such as lentiviral... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post... by experts in their respective fields: David Cameron, PhD - Director of Media Communications, Harvard... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...cognitive disabilities, problem behaviors, and a chronic feeling of hunger that can lead to excessive eating... -
Degrading DNA with Cascade-Cas3
TypeBlog Post...1). https://doi.org/10.1038/s41467-019-13226-x Cameron, P., Coons, M. M., Klompe, S. E., Lied, A. M., ... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog Post...AAV, but also form secondary structures that are prone to deletion from the transfer plasmid. The good ... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog Post...ligation efficiency and identification of mismatch-prone pairings. Using these 4 bp overhang observations... -
10 Basic tips for mammalian cell culture
TypeBlog Post... a well organized lab and contamination free environment for successful experiments: To clean air and ... -
Transferable Skills Guide: Managing a Team
TypeBlog Post...embrace them after one ended with the person in front of me saying “I’ve been doing some of X’s work for... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...expression. We highly recommend the use of an electronic pipettor with variable spacing (such as the Viaflo... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog Post...studying biosignature preservation in Mars analogue environments. Alejandro (Alex) Chavez is a member of ...