We narrowed to 653 results for: PPL
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TypeBlog Post...of CRISPR; site-directed mutagenesis generally applies to plasmids, but may also facilitate genome editing...
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Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...lessons learned in pursuing one technological application can benefit many others and accelerate the pace... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post...discounts, collaborations, access to internal award applications, etc. If this is the institute that can help... -
Hot Plasmids: Spring 2025
TypeBlog Post...popular choice for a variety of synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A.... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...to specific DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety... -
Plasmids 101: Restriction Cloning
TypeBlog Post...toward the cathode (- charge). When the voltage is applied across the gel, the DNA migrates toward the anode... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog Post...Variables: A Monte Carlo Simulation Study. Front. Appl. Math. Stat. Volume 2 (2016). doi.org/10.3389/fams... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...using. Contact Addgene ([email protected]), or the supplier of your vector, to obtain this information. Optimize... -
A History of Genome Engineering in Popular Culture
TypeBlog Post...extracting the DNA from fossilized amber and supplementing it with the DNA of present-day frogs via genetic... -
Quick Guide to All Things Lentivirus
TypeBlog Post...have been increasingly popular in fundamental and applied research since their first use in the early 90’... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...the user. This approach is useful for several applications as will be discussed below. The software could... -
Antibodies 101: Flow Cytometry
TypeBlog Post...protein composition. It is used for a variety of applications such as measuring target protein expression ... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog Post...recognition, and DNA cleavage. RNA loading Once supplied with a guide RNA — whether it’s an sgRNA or a ... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...the gene/insert. Pooled libraries are normally supplied as liquid DNA with all of the plasmids in the ... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...directed differentiation.” Specific cell media, supplements, bioactive small molecules, and growth factors... -
CRISPR/Cas9 FAQs Answered!
TypeBlog Post...with the newest CRISPR technologies and their applications can be exhausting. A quick search for "CRISPR... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...using an antibody validated for your protein and application of interest or validating an antibody yourself... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post... distinction of neighboring cells in imaging applications and the mapping of neuronal projections to their... -
Antibody Guide
TypeGuide...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...production and storage techniques, and explain common applications. Science... Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods...of other antibodies. This has useful research applications, and is covered in the following Isotypes section...signaling molecules and are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments... molecules that can be distinguished in your application. The indirect detection method uses an unconjugated... -
CRISPR Guide
TypeGuide... to various Cas enzymes have extended CRISPR applications to increasingly complex functions, including...understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR... Cas12a, which are available for specialized applications. Check out our list of additional Cas proteins...genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using...to help you select an optimized gRNA for your application (see: gRNA Design Software ). In addition to ...edits, they can be used for virtually any CRISPR application. Cas9 Orthologs While SpCas9 is the most commonly...require longer homology arms. Depending on the application, the donor DNA may be a single-stranded oligonucleotide...