We narrowed to 682 results for: RON

1. Addgene's Cancer Collection Pages Connect the Research Community

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   Blog Post

   ... circulatory and lymph systems are not ideal environments for many cell types. From proteins to pathways...

2. Google Forums Round Up: First Impressions of NgAgo

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   Blog Post

   ...et al. "DNA-guided genome editing using the Natronobacterium gregoryi Argonaute." Nature biotechnology ...

3. Plasmids 101: Optimizing Plasmid Yields

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   Blog Post

   ...improve plasmid stability and yield. If a plasmid is prone to recombination Use a strain optimized for plasmids...

4. SciComm: Taking Science to Elementary and Middle Schools

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   Blog Post

   ... with a good public school system that has made strong efforts in the last 5 years to incorporate more...

5. Viral Vectors 101: The Retroviral Lifecycle

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   ...Bacteria Concentrations in Indoor and Outdoor Air. Environmental Science & Technology Letters, 2(4), 84. https...

6. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   ...  Ashley Waldron...

7. Tips for arabidopsis transformation

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   ...Agrobacterium vectors for plant synthetic biology’, Frontiers in Plant Science, 4. doi: 10.3389/fpls.2013.00339...

8. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   Blog Post

   ...CRISPRko libraries are highly effective at identifying strong hits in genetic screens, they do have several limitations...

9. Plasmids 101: Colony PCR

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   ...product, then you know there could be something wrong with the PCR setup and/or the primer design. ...

10. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post

    ... proteins at once.  However, as Y2H screens are prone to both false positive and false negatives, these...

11. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    Blog Post

    ...collections. Many companies, such as Thermo Fisher and Micronic, provide 2D barcoded tubes. 1D barcodes are better...

12. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...Japanese science. I really miss the intellectual environment in the US in this regard. In addition, not many...

13. QC Sequencing Technologies at Addgene

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    ...., Milanova, D., Kerby, M. B., Snyder, M. P., & Barron, A. E. (2011). Landscape of Next-Generation Sequencing...

14. Plasmids 101: Shuttle Vectors

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    Blog Post

    ...slow-growing cells, particularly mammalian cells like neurons, may not replicate during the course of your experiment...

15. Twenty Years of Sharing: Expanding Accessibility

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    ...we launched the Addgene YouTube channel to add a strong visual component to our educational resources. ...

16. Overcoming the AAV Size Limitation for CRISPR Delivery

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    ... mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine et al developed...

17. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ (...

18. Viral Vectors 101: Preparing Pooled Libraries

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    ...protocols therefore differ from library to library. We strongly recommend following the protocols provided by ...

19. Antibodies 101: Immunoprecipitation

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    Blog Post

    ...References and resources References:  Björck L, Kronvall G (1984) Purification and some properties of streptococcal...

20. CRISPR-based Genome Editing Systems For Editing in E. coli

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    ...Escherichia coli Genome via the CRISPR-Cas9 System. Appl Environ Microbiol 81:2506–2514 . https://doi.org/10.1128...