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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 949 results for: ache

Showing: 621 - 640 of 949 results

  1. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...control? Addgene lists the primers used to obtain each result above the posted sequence in the "View Sequence...facility The corresponding primer sequence used for each reaction Tips and FAQs My sequence doesn’t match...

  4. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...We perform over 100 transformations each week at Addgene, so we need to be cost and time efficient. We...for at least a year when stored at -80oC.   For each new batch of competent cells, don’t forget to do...

  5. Ligation Independent Cloning

    Type
    Protocol
    ...the 3' end of the cut site until the first G is reached (shown in blue), at which point it will add back...transformation. Use 1-2 μl of annealing reaction for each transformation, following a standard protocol . ...

  6. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ...points to collaboration and delegating as keys achieving success. Chapter 9: Science by the Pint - We learn...knowledge and continuity - two lead organizers on each program, one junior and one senior.  To learn more...

  7. Science as Art

    Type
    Blog Post
    ...Albert G. Richards, a teacher of dental radiography, had focused his x-ray machine not on teeth, but on...

  8. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...in person. Joanne Kamens, Melina Fan, and Ken Fan each spoke about what Addgene means to them. Ken mentioned...The Addgene lab often works as a very well-oiled machine and this post was no exception - the rest of the...

  11. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...they would be disregarded - everyone was very approachable.     Fig. 1: Examples of DNA sequencing...was done through the “Addgene 101” series, where each team gives a presentation on what they do and how...

  12. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...property rights and handling your constructs once they reach you. As with all of our resources, we will continually... our website; towards the bottom right corner of each page there’s a blue icon displaying the word “Help...

  14. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...inquiries. The office is now focused on scientific outreach and community engagement. Information about contacting...Just a few weeks ago I became the newest Addgene Outreach Scientist - the first to be located in London ...

  15. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...could provide a streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind...prime editing with a site-specific recombinase to achieve insertion of long sequences. In their experiments...

  16. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... array is transcribed and processed into crRNAs, each including one repeat and one spacer. In bacteria...protospacers. Repeats: Bacterial DNA that appears between each spacer in the CRISPR array, distinguishing the foreign...

  17. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...This post was co-authored by Susanna Stroik and Rachel Leeson. Here at Addgene, we like to help share ...Bairoch from the Swiss Institute of Bioinformatics reached out to us to tell us about a resource called Cellosaurus...

  18. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...plasmids in a number of different experimental approaches. They first assessed the activation of different...Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT (2020) TRUPATH, an open-source biosensor platform...

  19. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...specific components that make up a plasmid and why each is important. Our “Plasmids 101” series designed...replication within a plasmid by recruiting replication machinery proteins Antibiotic Resistance Gene Allows for...

  20. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...a higher signal-to-noise ratio with a lower photobleaching rate. Given the power of SunTag, they attempted... ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule tracking...
Showing: 621 - 640 of 949 results