...plasmids and protocols needed for using them, are described below. Read our CRISPR 101 post for more information... the first publicly available collection of fluorescently tagged, human, induced pluripotent stem cell...

...lab determines a minimally functional MECP2 that rescues neurological defects of Rett syndrome. (Link opens...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...

...nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong, et. al, 2013 Science ...co-transfect in HR donor. Alternatively, a new system described recently is the double nickase system, with PX335...

... nuclear receptor subfamily 2, group E, member 3 ESCS, MGC49976, PNR, RNR, RP37, rd7 NR2F1 nuclear receptor...Resources You May Also Like: Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene Categories...immune response. Over 60 interleukins have been described. NK Cell Signaling Natural killer (NK) cells can...immune response. Over 60 interleukins have been described. Symbol Name Synonyms EBI3 Epstein-Barr Virus ...and standardized building blocks: 14 different fluorescent proteins, 10 mammalian promoters and enhancers...Plasmids 101: Monitoring Cell Mobility Using Fluorescent Proteins The Challenges of Cell Culture External...

...Find AAV enhancer plasmids for fluorescent labeling and recombination across brain regions and populations... enhancers for specificity and brightness of fluorescent labeling across brain regions and populations...

..., reverse primer pBluescriptKS TCGAGGTCGACGGTATC For pBluescript vector pBluescriptSK TCTAGAACTAGTGGATC...TCTAGAACTAGTGGATC For pBluescript vector pBMN 5' GCTTGGATACACGCCGC MMLV sequence, for inserts in pBMN retroviral vector...

...the particular conditions of the experiment as described in the associated article (listed below by PubMed...within promoters. When possible, the categories described on Addgene's CRISPR Plasmids and Resources page...

...Overlay Select Fluorescent Proteins 101 August 2017 | First Edition About Fluorescent Proteins 101 ... this eBook, you’ll find tips for choosing a fluorescent protein; information on FRET, biosensors, and...

...proximal promoter, and distal promoter. Below describes the specifics of these regions in eukaryotic cells...Promoters Used in Research Promoter Expression Description CMV Constitutive Strong mammalian promoter from...Promoters used in Research Promoter Expression Description T7 Constitutive but requires T7 RNA polymerase...

...include: Plasmids 101 CRISPR 101 Viral Vectors 101 Fluorescent Proteins 101 Science Career Guide Videos Watch...techniques. Topics include: Genome Engineering Fluorescent Proteins CRISPR Viral Vectors Plasmid Technology...

...lentiviral packaging methods. The three components described above (envelope, packaging, and transfer) are ...nomenclature of the plasmid backbone is meant to be descriptive of the components found within the backbone. ...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

...our Antibody Guide . ID Recombinant Antibody Description Source Species Isotype Reactivity Recommended...