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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 882 results for: NSI

Showing: 641 - 660 of 882 results

  1. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...our own repository data, giving us significant insight into which vectors are likely to be of most use...Swanson, L. E., & Fan, M. (2016). Practical Considerations for Using Pooled Lentiviral CRISPR Libraries...

  2. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...plasmids tend to be high copy number. Here, we will consider the replication mechanism of plasmids under stringent...plasmid to balance the total plasmid number.  Let’s consider pSC101. This plasmid is stringently controlled...

  3. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...with idea generation, try this thought exercise: Consider the issue as if there were no restraints on your...something completely unrelated to the issue and consider how it could be shaped to solve your problem. ...

  4. How to Be an Excellent Trainee

    Type
    Blog Post
    ...have, then bring them to your mentor. Pro tip! Consider physically walking through the protocol, by going...receive help until you’ve already started a time sensitive experiment. Ready to fly solo? Let your bench...

  5. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...editing field by making experimental workflows considerably easier, faster, and more efficient than previous...novice to expert-level researchers in achieving consistently successful CRISPR experiments. Jessica is a ...

  6. Hot Plasmids - August 2020

    Type
    Blog Post
    ...CRISPR/Cas Toolkit for plants includes 95 plasmids consisting of CRISPR/Cas nucleases, base editors, gRNA backbones...activation-based norepinephrine (GRABNE) sensors allow for sensitive and high spatiotemporal measurements of norepinephrine...

  7. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...partitioning system in the bacteria, but generally it consists of a centromere-like region on the plasmid, a ...2015) Imaging centromere-based incompatibilities: Insights into the mechanism of incompatibility mediated...

  8. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...from many other types of cancer. Mesothelioma is considered an orphan cancer; it is rare disease with only...non-resectable mesothelioma by the National Comprehensive Cancer Network, a coalition of the top 27 cancer...

  10. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...this interaction will give you an idea of how responsive and supportive the ELN team is. Once you’ve made...information. If an employee leaves the institution, the transition is smooth as all data is organized within an ...

  12. Five Popular Model Organisms

    Type
    Blog Post
    ...great model organism because they are easy and inexpensive to grow and maintain in the lab, have a short... worm, Caenorhabditis elegans, has been used extensively as a model organism since the 1970s when Sydney...

  14. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...into the mouse zygote (Qin et al., 2015).  When inside the zygotes, the gRNA will seek out its target ... Ong ST, Bergstrom DE (2018) Viable Mice with Extensive Gene Humanization (25-kbp) Created Using Embryonic...

  15. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ... that have negative impacts on fitness. One consideration when making a transgenic cell line with the ...the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon system can...

  16. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ... Summers, 1998). Multimeric plasmids are also considered to be less stable and more prone to being lost...the supercoiled plasmid monomer, and at <6 kb, consistent with the size of a supercoiled plasmid dimer....

  17. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...regions you want to evolve. With this added layer of insight, you can shrink your mutant library and increase... Biotin. The antibodies developed by IPI are extensively characterized and validated, with the application...

  18. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...training. Similarly, there are several factors to consider when selecting a postdoc mentor and lab. These... having sufficient data to write a manuscript. Consider questions like: What figures are missing? For ...

  20. Colony Formation Titering Assay

    Type
    Protocol
    ... in the lab by heating to 56 °C for 30 min. Considerations Before You Start The health of the target cell...pipetting or inverting. Aliquot 1.35 mL of the cell suspension into each well of the 6-well dish directly on... 48–72 h. Pro-Tip Different cells may be more sensitive to lentivirus or to polybrene, in which case this...
Showing: 641 - 660 of 882 results