We narrowed to 682 results for: RON

1. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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   Blog Post

   ...Synergistic Activation Mediator complex, generally the strongest activator, consists of a dCas9-VP64 fusion and...

2. Bioinformatics at Addgene

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   Blog Post

   ...our pipeline on a local computer or in a cloud environment. Given the throughput necessary to keep up with...

3. Transferable Skills Guide: Creativity

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   ...most prominent idea, and instead building an environment where everyone has their ideas heard.  So how...

4. Antibodies 101: Immunoprecipitation

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   ...References and resources References:  Björck L, Kronvall G (1984) Purification and some properties of streptococcal...

5. How to Be an Excellent Trainee

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   ...what you don’t know – i.e., if they use a term or acronym you don’t know, or if they assume you have done...

6. Viral Vectors 101: Preparing Pooled Libraries

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   ...protocols therefore differ from library to library. We strongly recommend following the protocols provided by ...

7. CRISPR-based Genome Editing Systems For Editing in E. coli

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   ...Escherichia coli Genome via the CRISPR-Cas9 System. Appl Environ Microbiol 81:2506–2514 . https://doi.org/10.1128...

8. CRISPR-mediated Plant Base Editors

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   ...promoter driven expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple...

9. How to Write a Scientific Review Article

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   Blog Post

   ...think this is represented by factorials. In environmental science and ecology, this can be represented...

10. Mesothelioma - Causes, Symptoms, and Treatment

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    Blog Post

    ...be exposed to asbestos. However, as the U.S. Environmental Protection Agency has noted, there is no safe...

11. Getting the Most from Your Lentiviral Transduction

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    ...110.18 (2013). 7306-11. Pubmed PMID: 23589850. 9. Cronin, J., Zhang, X.Y., Reiser, J. “Altering the tropism...

12. Plasmids 101: Control Plasmids

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    ...viable? Or cytotoxic? How and where did things go wrong? The use of proper control plasmids would address...

13. Tips for Using FRET in Your Experiments

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    Blog Post

    ...in many FRET experiments. In addition, other environmental factors, such as pH or autofluorescence background...

14. Antibodies 101: Introduction to Immunofluorescence

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    ... Ashley Waldron...

15. CRISPR 101: Homology Directed Repair

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    ...method is single strand annealing (SSA), an error prone mechanism that notably does not require a donor ...

16. Evolution of Lab Techniques

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    ...referred to as sequence DNA as it synthesized by electronically recording fluorescence activity as fluorescently...

17. CRISPR 101: Epigenetics and Editing the Epigenome

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    ...Created with BioRender.com. Tet1 demethylase Ronggui Hu’s lab has created pdCas9-Tet1-CD for targeted...

18. CRISPR 101: Off-Target Effects

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    ...Off-target effects in CRISPR/Cas9 gene editing. Front Bioeng Biotechnol. 2023 Mar 9;11:1143157. doi: 10.3389...

19. Antibodies 101: Multiplex Immunofluorescence

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    Blog Post

    ...you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed over 65...

20. Plasmids 101: Dimers and Multimers

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    ... are also considered to be less stable and more prone to being lost in bacterial cells compared to monomers...