...protocols therefore differ from library to library. We strongly recommend following the protocols provided by ...

...slow-growing cells, particularly mammalian cells like neurons, may not replicate during the course of your experiment...

...we launched the Addgene YouTube channel to add a strong visual component to our educational resources. ...

...Synergistic Activation Mediator complex, generally the strongest activator, consists of a dCas9-VP64 fusion and...

...Repetitive sequences Restriction sites Sequences prone to create secondary structures in RNA transcripts...

...et al. show that genome-wide CRISPR screens are prone to some of the same flaws as RNAi screens, such ...

...110.18 (2013). 7306-11. Pubmed PMID: 23589850. 9. Cronin, J., Zhang, X.Y., Reiser, J. “Altering the tropism...

...viable? Or cytotoxic? How and where did things go wrong? The use of proper control plasmids would address...

...in many FRET experiments. In addition, other environmental factors, such as pH or autofluorescence background...

...Constitutive Strong mammalian promoter from human cytomegalovirus EF1a Constituitve Strong mammalian promoter...have complex systems of gene regulation, like operons. Operons are a cluster of different genes that are ...together. Operon regulation can be either negative or positive. Negative repressible operons, are normally...transcription. The Lac operon in bacteria is an example of a negatively controlled operon. A positive repressible...repressible operon works in the opposite way. The operon is normally transcribed until a repressor/corepressor...Promoter from Lac operon araBad Inducible by arabinose Promoter of the arabinose metabolic operon trp Repressible...human elongation factor 1 alpha CAG Constitutive Strong hybrid mammalian promoter PGK Constitutive Mammalian...

... Ashley Waldron...

...method is single strand annealing (SSA), an error prone mechanism that notably does not require a donor ...

...referred to as sequence DNA as it synthesized by electronically recording fluorescence activity as fluorescently...

...Created with BioRender.com. Tet1 demethylase Ronggui Hu’s lab has created pdCas9-Tet1-CD for targeted...

...you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed over 65...

...Off-target effects in CRISPR/Cas9 gene editing. Front Bioeng Biotechnol. 2023 Mar 9;11:1143157. doi: 10.3389...

... are also considered to be less stable and more prone to being lost in bacterial cells compared to monomers...

...and LIIbeta F 1-2 RNAi, which allows assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial...

...Central PMCID: PMC320540. 10. Goffin, Colette, Veronique Bailly, and Walter G. Verly. "Nicks 3′ or 5′ to...

... generated by directly injecting DNA into the pronuclei of fertilized eggs, which leads to random incorporation...