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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 960 results for: REP

Showing: 661 - 680 of 960 results

  1. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...sequence. The TIDE software will generate a graph representing all insertions and deletions identified within...Cas9 combo and are hoping for homology-directed repair events, or maybe you’re performing a single base...TIDER can screen for specific homology-directed repair events and small edits (Brinkman, et al., NAR)....

  2. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...depend on the target and sample type. Sample Preparation This step can involve multiple smaller steps...Preservation and Imaging Similar to Sample Preparation, this step can involve multiple smaller steps...especially if you work in an organism that is underrepresented in the biomedical research community. You ...

  3. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...Below is a step-by-step overview of the protocol: Prepare ssDNA template: The wild-type plasmid backbone ...means that if the BbvCI enzyme used for ssDNA preparation nicks the anti-sense strand, then the ssDNA template...Pooled Libraries to Generate Mutant Populations Use REPLACER Mutagenesis to Mutate Your Plasmids Resources...

  4. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ... the Confetti mouse (Snippert et al., 2010), a reporter mouse that can be used to track any cell type ...XFP expression in the Ubow mouse Another murine reporter animal, the Ubow mouse (Ghigo et al., 2013), was...dermis and identify that Langerhans cells are replenished through the proliferation of specific, and not...

  5. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...activation loop do not fully activate MEK1. In fact, replacing just one of the MEK1 phosphomimetic residues in...to eliminate all 321 amber stop codons (TAG), replacing them with TAA (7). This modification allowed the...expression vectors, the codon for the amino acid to be replaced with phosphoserine must be recoded to ‘TGA’ using...

  8. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...responsible for guiding new deposits into the repository, performing quality control, and providing technical...listening to Simon & Garfunkel’s “I Am A Rock” on repeat in my bedroom. I realized I could and should be...like kudzu. Think of a soccer player who gets a reputation for taking risky shots at the goal rather than...

  9. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...corresponding author publications (published, not in preparation) A history of successful funding (fellowship ...spurious yet intriguing findings that fail to replicate. Plan experiments logically and strategically ...perform, and interpret experiments. You'll be better prepared for your future intended career and you'll become...

  10. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...tag your protein of interest with a fluorescent reporter on the N- or C-terminus of the protein. One downside...depends on a variety of factors including sample preparation, staining procedures, and controls. To prevent...multicolor flow cytometry experiment, users must also prepare sets of controls to aid with compensation. Compensation...

  11. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...Clustered Regularly Interspaced Short Palindromic Repeats, in Pubmed returned 728 articles (3/12/2014). With...Gene Conversion Tracts from Double-Strand Break Repair in Mammalian Cells. Molecular and Cellular Biology.... (2013). One-Step generation of mice carrying reporter and conditional alleles by CRISPR/CAS-Mediated...

  13. Suggest A Plasmid You'd Like to Find at Addgene

    Type
    Blog Post
    ...Can’t find a plasmid you’re looking for in the repository? Let us know! You can notify us to reach out ...depositing, we hope this campaign will expand the repository in ways that will be most useful to you, the ...

  14. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...Now we have over 8,000 CRISPR plasmids in the repository that have been shared over 140,000 times to over...applications (such as visualize, screen, activate, and repress) on Thursday. We’d also like to see what you think...

  15. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    ...fields.  Addgene is a global nonprofit plasmid repository. Over 2,000 labs have deposited plasmids to Addgene...Zhang acknowledge the role of data and materials repositories in aiding the dramatic spread of the Cas9 technology...

  16. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...el acceso global a los materiales de nuestro repositorio. Este increíble regalo permitirá que más personas...help increase global access to materials in our repository. This incredible gift will allow more people ...

  17. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...introduced us to RUBY-red, a bright red pigment reporter for plants.  Cas7-11 and PASTE: Omar Abudayyeh...excellent Q&A from Tim Miles on everything from AAV prep to targeting to animal models. It was one of our...

  18. Call for Guest Bloggers

    Type
    Blog Post
    ... a trick to get the highest yield out of your minipreps? If so, we’d love to have you write for us! Click...molecular biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career development...

  19. Our New Antibody Mascot is....

    Type
    Blog Post
    ...our new antibody mascot, who's joining us in preparation for our upcoming affinity reagent distribution...quest. Keep an eye out for Abi as they help us prepare for our upcoming antibody launch in late March!...

  20. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...longevity. 10:56 - 12:00 : How Alex's work in DNA repair informed his decision to work with CRISPR during...Koeris Explains His Path from Grad Student to Entrepeneur Find Resources to Help You Choose Your Career...
Showing: 661 - 680 of 960 results