We narrowed to 869 results for: SOM

...them back to wild type), differentiate them into somatic cells affected by the disease and study what’s ...guide RNA binding site works well. However, since some guide RNAs may tolerate a mismatch in the binding...

...plant breeding approaches, these techniques are cumbersome, often requiring several rounds of selection ...functionality of the CRISPR system in most organisms, some plant-specific changes to CRISPR components are ...

...serotypes have a primary cellular receptor and sometimes one or multiple coreceptors, which may be present...present on different cell types and sometimes vary between target species or even strains. Pro tip! Getting...

...of the vector. In order to reduce the chances of some clones being outcompeted by others in liquid culture...extraction reagent (BPER from Thermo works well) with some added lysozyme, DNAse I, and protease inhibitor ...

... was really due to the intended edit rather than some passenger variant that came along with the single...likely to be active, not only for SpCas9 but also for some other Cas enzymes. It seems to be the case that ...

...construction of plasmid vectors and/or rely on cumbersome in vitro synthesis of single guide RNAs (sgRNAs...of knock-in experiments depends on many factors. Some of these include: Most importantly, the crRNA...

...planning your chemogenetics experiment, here are some things to consider: Activation or Inhibition? Do...with cell-type specific promoters. Table 4 lists some common promoters found in chemogenetic receptor ...

...brief overview of the wild-type opsin of each and some examples of relevant variants. This list is not ...pick both an opsin and a delivery system. Here are some key factors to consider: Excitation or inhibition...

...work than using established methods and minimizes some of the technical issues associated with high levels...Classic genetic engineering methods enabling chromosomal integration of sequences in bacteria are time-consuming...

...'s out there, Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses...

...We’re so excited to share some good news with you: with contributions from depositors around the globe...

...Twitter, so keep an eye out there for a chance to win some cool Addgene swag.  And, of course, to all our depositors...

...and even those who might just need a refresher on some best practices for introductory science techniques...

...your best ideas because the finalists will receive some newly designed Addgene swag. Have fun! Suggest a...

...Every year, we like to add in some holiday cheer to our blog and social media. This year, we're doing...

...we have added new sections to share and describe some incredible new CRISPR technologies developed and...

... asking you to help us make the decision between some meaningful names…. Watch the video to find out the...

...Addgene's past. Framed photos and slideshows shared some of the moments we captured over the years, including...

...Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth day of CRISPR, Addgene...

...Every year we celebrate the 12 Days of CRISPR with some of the latest and greatest in CRISPR tools and resources...