We narrowed to 878 results for: SOM

...Every few months we highlight some of the new plasmids, antibodies, and viral preps in our repository...number of cell types at different genomic loci — in some cases, over 20-fold higher editing than PEmax. The...

...the independent variable you are testing and not some other unintended factor. Control plasmids are used...different days, etc, but outside constraints may impose some limitations and you should be cognizant of these...

...plant breeding approaches, these techniques are cumbersome, often requiring several rounds of selection ...functionality of the CRISPR system in most organisms, some plant-specific changes to CRISPR components are ...

...bind discrete subunits or particular subunit pairs. Some of the antibodies in the collection are able to ...hybridoma antibodies developed in the Springer lab. Some are rabbit immunoglobulin G1 (IgG1) chimeric antibodies...

...configurations can generate functional scFvs; however, some individual scFvs performing better in one configuration... of screening. Created with Biorender.com.   Some phage display libraries screen antibody genes from...

...them back to wild type), differentiate them into somatic cells affected by the disease and study what’s ...guide RNA binding site works well. However, since some guide RNAs may tolerate a mismatch in the binding...

...immunostaining of the truncated YFP. In Thy1-Brainbow mice, some neurons expressed as many as 16 copies of the injected...cell, even axons of up to 100 microns in length. Some challenges of the Brainbow system are that it does...

...nucleus, or visualizing DNA instead of proteins. Some stains can diffuse through the cell membrane freely...depending on the location of the targeted markers. Some commercially available fix/perm kits allow for labeling...

...of the vector. In order to reduce the chances of some clones being outcompeted by others in liquid culture...extraction reagent (BPER from Thermo works well) with some added lysozyme, DNAse I, and protease inhibitor ...

...serotypes have a primary cellular receptor and sometimes one or multiple coreceptors, which may be present...present on different cell types and sometimes vary between target species or even strains. Pro tip! Getting...

... was really due to the intended edit rather than some passenger variant that came along with the single...likely to be active, not only for SpCas9 but also for some other Cas enzymes. It seems to be the case that ...

...construction of plasmid vectors and/or rely on cumbersome in vitro synthesis of single guide RNAs (sgRNAs...of knock-in experiments depends on many factors. Some of these include: Most importantly, the crRNA...

...Stewart-Ornstein et al., 2025), DNA-FISH for chromosomal structural variant analysis, targeted sequencing...PM577a showed no detectable off-target editing or chromosomal abnormalities. Together, these data illustrate...

...have the same backbone, but contain unique inserts. Some libraries target or express genes representing the...contact us at [email protected] for assistance. For some libraries, plasmid DNA can be delivered directly...

...planning your chemogenetics experiment, here are some things to consider: Activation or Inhibition? Do...with cell-type specific promoters. Table 4 lists some common promoters found in chemogenetic receptor ...

...work than using established methods and minimizes some of the technical issues associated with high levels...Classic genetic engineering methods enabling chromosomal integration of sequences in bacteria are time-consuming...

...'s out there, Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses...

...We’re so excited to share some good news with you: with contributions from depositors around the globe...

...and even those who might just need a refresher on some best practices for introductory science techniques...

...your best ideas because the finalists will receive some newly designed Addgene swag. Have fun! Suggest a...