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  1. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...bind discrete subunits or particular subunit pairs. Some of the antibodies in the collection are able to ...hybridoma antibodies developed in the Springer lab. Some are rabbit immunoglobulin G1 (IgG1) chimeric antibodies...

  2. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... was really due to the intended edit rather than some passenger variant that came along with the single...likely to be active, not only for SpCas9 but also for some other Cas enzymes. It seems to be the case that ...

  3. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...of the vector. In order to reduce the chances of some clones being outcompeted by others in liquid culture...extraction reagent (BPER from Thermo works well) with some added lysozyme, DNAse I, and protease inhibitor ...

  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...work than using established methods and minimizes some of the technical issues associated with high levels...Classic genetic engineering methods enabling chromosomal integration of sequences in bacteria are time-consuming...

  10. 2022 Depositor Week

    Type
    Blog Post
    ...Twitter, so keep an eye out there for a chance to win some cool Addgene swag.  And, of course, to all our depositors...

  11. The Twelve Days of Antibodies

    Type
    Blog Post
    ...Every year, we like to add in some holiday cheer to our blog and social media. This year, we're doing...

  12. CRISPR Guide Update

    Type
    Blog Post
    ...we have added new sections to share and describe some incredible new CRISPR technologies developed and...

  14. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth day of CRISPR, Addgene...

  15. The 12 Days of CRISPR

    Type
    Blog Post
    ...Every year we celebrate the 12 Days of CRISPR with some of the latest and greatest in CRISPR tools and resources...

  16. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...Addgene's past. Framed photos and slideshows shared some of the moments we captured over the years, including...
Showing: 661 - 680 of 861 results