We narrowed to 757 results for: Dos

Plasmids can be under either stringent regulation of replication or relaxed regulation of replication. In this blog post, we explain how stringent regulation works.

Looking for a plasmid that can thrive in a number of different bacteria? Learn about broad host range plasmids in this post!

If flow plots seem confusing, our guide to understanding them is here to help!

Pseudotyping is a method use to introduce a viral envelope protein from another virus to restrict or broaden host cell. Get the what, why, how of pseudotyping in this article.

Discover the history of Rett Syndrome research, the role of the MECP2 gene, and some of the latest breakthrough therapeutic approaches for Rett Syndrome.

Learn how a new episomal plasmid-based method can be used to efficiently reprogram blood cells to induced pluripotent stem cells

Learn how to optimize CRISPR-activated homology directed repair to make targeted edits in mammalian cells.

One-pot saturation mutagenesis can quickly generate libraries of mutant plasmids for phenotypic screening. Learn more from SciComm intern Beth Kenkel.

Use site directed mutagenesis to insert small mutations into your plasmid of interest and follow these tips for a smooth process with easy validation.

The next post in the Plasmids 101 series focuses on the promoter region, the element responsible for initiating the transcription of your insert into RNA.

Protocol for how to perform a diagnostic digest to verify plasmid size, verify insert orientation, and more.

These lab safety guidelines provide steps to ensure you are working in BSL-1 and BSL-2 labs safely.

This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells.

Curious about systemic capsids? Looking for one to test in your experimental system? This 101 is an introduction to systemic capsids uses and types!

Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

Protocol for Inoculating a Bacterial Culture

This post describes CRISPR-derived base editors that catalyze base transversions, such as purines like A or G to pyrimidines like T or C.

Optogenetics aren't just for neuroscientists! In this post, we review optogenetic tools for cytoskeleton and membrane control in cells.

Get a behind-the-scenes look at how we've adapted video production during COVID-19.

With the limitations of qRT-PCR based COVID-19 testing, scientists develop CRISPR-based diagnostic tools for COVID-19.