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We narrowed to 768 results for: Dos

Showing: 681 - 700 of 768 results

  1. CRISPR Guide

    Type
    Collection
    ...to G (or T to C) change. Adenosine DNA deaminases do not exist in nature, but have been created by directed...have been developed for inhibition using dCas9. How Do You Use a CRISPR Library? CRISPR libraries from Addgene...alterations, such as small mutations or inversions, than do large deletions generated by Cas9 systems. Cas3 must..., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J.,...

  2. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...strategy, sometimes it cannot be avoided. If you do have to do so, there is no way to control which orientation...vector backbone and therefore the only thing left to do is identify the clone(s) in which the insert is in...

  3. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...bacteria containing that plasmid from bacteria that do not contain it by artificial selection (i.e. growing...growth (bottom) Loosely close the cap on the bottle (do NOT close all the way or the bottle may explode!)...concentration ( see table below ). Note: If you intend to do a mini-prep you will usually want to start 2 mL in...

  4. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...wear it the whole time you are working in the lab. Do not eat, drink, chew gum, or apply makeup in the ...way. It is the easiest way to ensure that you’re doing your work in the safest way possible. Protecting...this SOP in another location, please note that you do so at your own risk; you should ensure that any local...

  5. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...filter or centrifugation at >500 x g for 5 minutes. Do not use the medium if the cells are overly confluent... solution into each well of a 96-well plate. By doing this, you are seeding the plate at an average density...After ~7 days, scan the plate for cell growth. If you do not observe any colonies, continue incubating the... one colony. Wells with more than a single colony do not contain a monoclonal population and should be...expansions (monoclonal, 1-5). Wild-type A549 cells do not express Cas9 and are included as a control (WT...

  6. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...regularly Do not over or under-grow your cells. Thaw a new vial of cells after 20-30 passages. Do not add...determine the optimal dose of selective reagent for your target cell line. To do this, treat target cells...surviving cells in the culture, so it is important to do regular media changes and maintain optimal growth...cells with a range of doses of antibiotic and determine the lowest dose that kills all of the cells. Prepare...should be changed every 2–3 days to maintain the dose of antibiotic, which may not be stable at 37 °C....

  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...caption courtesy of Challis et al., 2022. Where do they come from? Systemic capsids are typically derived...efficacy can vary depending on species. LY6A and LY6C1 do not have known homologs in NHPs, and capsids that...mouse strains). AAV-PHP.eC, AAV9-X1.1 and AAV1-X1 do not display strain-specific tropism and can be used...2023). High viral titer High vector genome (vg) doses are recommended for all systemic capsid applications... AAV-PHP.eB is typically administered at a total dose between 1 x 1011 and 5 x 1011 vg per animal (i.e...mL titer), while AAV-PHP.S is typically used at a dose between 3 x 1011 and 1 x 1012 vg*. Most systemic...capsids are easy to produce in high titer, but higher doses (>1 x 1012 vg total in mice) may increase the chances...

  9. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...utmost priority for countries around the world and to do so, the WHO recommends one strategy: testing, tracking...Servellita V, Singh J, Miao X, Streithorst JA, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu ...

  10. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...your target base in the protospacer sequence. If you do not find a suitable ‘NGG’ PAM at your target genomic...

  13. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...processes in different cell types. One great way to do this is to use CRY2-CIB1 clustering. Fuse either ...provided CIB constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD...

  15. Evolution of Lab Techniques

    Type
    Blog Post
    ...comparison. These next-generation sequencing techniques do not typically rely on Sanger sequencing but instead...

  19. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...Gently mix sample (do not vortex) Incubate 30 min at 37 °C Transfer to ice ** Critical: do NOT treat your ...plates) and store at -20 °C. Once a standard is thawed do not freeze it again but store at 4 °C and use within... duplicate Load 5 μL of each sample in duplicate. Do not forget to include a no template control ( NTC...

  20. Protocol - How to Streak a Plate

    Type
    Protocol
    ...broad stroke. Only touch the surface of the plate, do NOT dig into the agar. Another very popular technique...bacterium. If the bacterial growth is too dense and you do not see single colonies, re-streak onto a new agar...
Showing: 681 - 700 of 768 results