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We narrowed to 729 results for: STO

Showing: 681 - 700 of 729 results

  1. Plan Your Experiment

    Type
    Guide
    ...using CRISPR . Browse Addgene's collection of in-stock lentiviral preps . AAV vectors AAV vectors offer...overcoming the AAV size limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged on Request service...paired with restriction enzymes for further customization Usually paired with a sequencing method Can’...

  2. CRISPR Guide

    Type
    Guide
    ...insertions, or frameshift mutations leading to premature stop codons within the open reading frame (ORF) of the... cytosines in a gene’s promoter or by inducing histone acetylation or demethylation. The most common modifiers... green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence microscopy...gene by shifting the ORF and/or creating premature stop codons NHEJ N on- H omologous E nd J oining; A DNA...

  3. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...transfection, you could use 1 µl of 10 µM oligo stock for each well on 24-well plate, co-deliver into ...

  6. Promoters

    Type
    Guide
    ...factors and histones (proteins that package DNA into nucleosomes) also bind the TATA box. Histone binding ...

  8. Chemogenetics Guide

    Type
    Guide
    ...phospholipase C, which releases intracellular calcium stores. There are currently three Gq DREADDs based on ...doi.org/10.1016/j.neuron.2015.03.065 PMID: 25937170 Weston, M., Kaserer, T., Wu, A., Mouravlev, A., Carpenter...

  9. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...capsids for more information. Browse Addgene’s in-stock viral preps . AAV retrograde (AAVrg) Directed evolution...Packaging Services Packaged-on-Request AAV Preps In-Stock AAV Preps Resources on addgene.org AAV Plasmids ...

  11. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ... For long term storage of the bacteria, you can proceed with creating a glycerol stock . You can now isolate...2 0. Addgene recommends making 1000X stock solutions and storing aliquots at -20 °C. To use, dilute your...media, add 100 μL of a 100 mg/mL ampicillin stock (1000X stock) to 100 mL of LB. Antibiotic Concentrations...Agar Plate Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids Recovering Plasmid DNA from...temperature. Now screw on the top of the bottle and store the LB at room temperature. When ready to grow your...growth (bottom). Preparing Antibiotics Create a stock solution of your antibiotic. Unless otherwise indicated...

  12. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...ng/µL stock into 900 µL PBS in a microfuge tube and vortex. 1 ng/µL : Add 450 µL of 2 ng/µL stock into ...multichannel pipette, add 100 µL of TMB Stop Solution directly to the wells to stop the reaction. Measure the absorbance...different incubation options available. Caution Use the Stop Solution in a well ventilated area and avoid breathing...tube and vortex. 0.5 ng/µL : Add 450 µL of 1 ng/µL stock into 450 µL PBS in a microfuge tube and vortex. .... 0.25 ng/µL : Add 450 µL of 0.5 ng/µL stock into 450 µL PBS in a microfuge tube and vortex. 0.125 ng/...ng/µL : Add 450 µL of 0.25 ng/µL stock into 450 µL PBS in a microfuge tube and vortex. 0 ng/µL : 450 µL...concentrations of HRP yield a greenish solution. Stop the reaction before any wells display a green product...

  13. Lentivirus Production

    Type
    Protocol
    ...sterile tubes. Store the tubes at -20 °C or -80 °C. After thawing, the solution can be stored at 4 °C for... the harvested media to a polypropylene storage tube and store at 4 °C between harvest. Centrifuge the... of heat-inactivated FBS and 5 mL of glutaGRO. Store at 4 °C. Pro-Tip Different brands and lots of FBS...months, discard the tube and thaw a new working stock. The optimal mass DNA:mass PEI ratio will need to... to batch variation when making the PEI working stock, therefore the ratio of μg DNA:μg PEI needs to be....45 μm PES filter. The viral supernatant can be stored at 4 °C for several hours but should be aliquoted...aliquoted and snap frozen in liquid nitrogen and stored at -80 °C as soon as possible to avoid loss of titer...

  14. Pipetting Protocol

    Type
    Protocol
    ...multiple “stops” on the plunger, where you feel resistance while pressing down on the pipette. Stop at the...the first “stop.” The first stop expels enough air from the pipette for an accurate measurement in the ...way to the second stop to release the liquid. Pro-Tip Dispense until the first stop, wait, and then dispense...the next step. The second stop is meant to expel excess liquid from the tip once you’ve reached step 8. ... to its resting position. Wait for the liquid to stop flowing into the pipette tip. Liquids of various...dispense to the second stop to expel any residual liquid in the tip. Before releasing the plunger, lift...

  15. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ... 2.5 μL Forward Primer (10 μM stock) 2.5 μL Reverse Primer (10 μM stock) 36.8 μL Sterile dH 2 O 0.2 μL... 2.5 μL Forward Primer (10 μM stock) 2.5 μL Reverse Primer (10 μM stock) 0.2 μL Taq DNA Polymerase (5 ...making a 1:10 dilution of the stock. For example, add 100µl of primer stock to 900µl of dH 2 O. Tips and...liquid, normally sterile dH 2 O. To make a 100uM stock of any primer, add a number of µl of dH 2 O equal...38.5nm, add 385µl of water. After making your 100uM stock, immediately make a working concentration of each...to supplement the amount supplied by the buffer stock, where it is known to form a gradient when frozen...

  16. Transfection for Recombinant Antibodies

    Type
    Protocol
    ... A6279 Before Starting Warm the DNA and working stock of PEI-MAX to room temperature before use, and warm...sterile 0.22 µm filter. Prepare 10 mL aliquots and store the solution at -20 °C. 1 mg/mL PEI-MAX Add 1 g ... through a 0.22 µm filter. Prepare aliquots and store at -20 °C until use. BCD TFX 1000 mL BalanCD HEK293...mL 200 mM Glutagro Do not add selective reagents Store at 4 °C until use. We suggest preparing fresh solutions...500 mL BalanCD HEK293 Feed 20 mL 200 mM Glutagro Store at 4 °C until use. We suggest preparing fresh solutions... the second tube of 6 mL BCD TFX (for a 1 mg/mL stock solution of PEI-MAX, 450 µg = 450 µL). Cap the tube...our Recombinant Antibody Purification Protocol ). Store the supernatant at 4 °C until ready to use....

  17. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ... plates) and store at -20 °C. Once a standard is thawed do not freeze it again but store at 4 °C and use...standard curve plasmid (2 x 10 9 stock made in step #1): Volume of 2 x 10 9 stock or previous dilution (μL) ...Water 4.7 μL 470 μL Procedure Prepare a plasmid stock of 2 x 10 9 molecules/μL to generate a standard ...79.8 = 1.25 μL Therefore we need to dilute 1.25 μL stock into 98.74 μL H 2 0 Pro-Tip Once a validated standard...Total dilution Dilution 1 (DNase step) 5 uL AAV stock 45 uL 10X 10X Dilution 2 5 uL Dil. 1 95 uL 20X 200X...

  18. Pouring LB Agar Plates

    Type
    Protocol
    ...your plates are ready for use or storage at 4 ℃. You should not store your plates for longer than 1 month...Antibiotic Concentrations Antibiotic Recommended Stock Concentration Recommended Working Concentration ...to the molten gel mix, you should create a 1000x stock solution. For example: If you’ll be preparing plates...concentration of 100 ug/mL ampicillin, you should make a stock solution of 100,000 ug/mL (100 mg/mL). Simply measure...solidifies in the bottle while you’re pouring, you should stop pouring and re-make the gel-mix. If you’re making...material to reduce condensation. The plates are then stored at 4 ℃ until use. Once your plates have solidified...

  19. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...to a polypropylene storage tube. The media contains your lentiviral particles. Store at 4°C. m. Add 5 mL...may be stored at 4°C for a few days, but should be frozen at -20°C or -80°C for long-term storage. TIP:...concentration of 100 μg/mL antibiotic. Pour plates and store at 4°C. Hexadimethrine Bromide (Polybrene) Prepare...catalog #H9268) in 0.9% NaCl. Autoclave to sterilize. Stock solution is stable at 4°C for up to one year. The...stable at 4°C for several years. Protamine Sulfate Store protamine sulfate (MP Biomedicals catalog #194729...soluble in cold water. Puromycin Prepare a 50mg/mL stock solution of puromycin (Sigma-Aldrich catalog #P8833... Sterilize by passing through a 0.22 μm filter. Store aliquots at -20°C. I.3. Warranty Information Addgene...

  20. General Transfection

    Type
    Protocol
    ...filter. Aliquot 50–100 µL and store at -20 °C. Aliquots can be thawed and stored at 4 °C prior to use. Thawed...into sterile tubes. Store the tubes at -80 °C. After thawing the solution can be stored at 4 °C for up to...heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and lots of FBS...months, discard the tube and thaw a new working stock. Considerations Before You Start The health of the...
Showing: 681 - 700 of 729 results