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Addgene

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We narrowed to 958 results for: Tes

Showing: 701 - 720 of 958 results

  1. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...reaction requiring luciferin, ATP and oxygen as substrates. Production of photons by this bioluminescent...of Photon Generation Slower Faster Cofactors/Substrates Required Not Required Signal Strength Lower...

  2. Video Library

    Type
    Protocol
    ...Making LB Agar Plates Create plates to culture bacteria in the lab Pouring LB Agar Plates Protocol Streaking...Bacteria on Plates Isolate single bacterial colonies on an agar plate Streaking Bacteria on Plates Protocol...CRISPR Protocol Over-Agar Antibiotic Plating Create plates to culture bacteria in the lab Over-Agar Antibiotic...

  4. Clear and Accessible Writing

    Type
    Blog Post
    ...supernatant through each layer. The right panel illustrates a gradient tube before and after ultracentrifugation...ultracentrifugation. The viral supernatant migrates through increasingly dense layers until the macromolecule density...

  5. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...security-critical bugs, and contribute to constant updates of the software. In hardware, this process is often...are as follows: Detailed documentation, which facilitates the reproduction of the structure in a similar...

  6. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...small molecule realm is typically not feasible to test in any given screening experiment. Thus, a limited...require there to be a culture condition which eliminates manipulations that do not affect the function...

  7. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ... and have worked closely with us to functionally test our virus. Ed Boyden’s lab from MIT and Alla Karpova... methods, performs extensive quality control, generates  educational materials, and provides viral vector...

  8. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...site of interest, which nCas9 nicks and then dissociates from. PolI3M then binds the nicked DNA, and extends..., where it nicks its target DNA (left). Cas9 dissociates and PolI binds (middle) and extends from the ...

  9. Antibodies 101: ChIP

    Type
    Blog Post
    ...  Fig. 1: Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay, let’s...celebrating a successful ChIP experiment! Image courtesy of https://www.wannapik.com/   Even though...

  10. Hot Plasmids - February 2022

    Type
    Blog Post
    ...2: comparison of Cas 9 vs. Cas 12a expression cassettes when encoding multiple guides. While two guides... and fusion proteins, such as Cas9 and Cas12a, creates challenges for in vivo delivery and cell engineering...

  11. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...parts: the receptor itself and the G-proteins it associates with.  Receptor: GPCRs are also known as seven...transducing receptors in the brain they are ideal candidates for use in chemogenetics. To create chemogenetics...

  12. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ... including the recent homepage and search tool updates. Now, let’s take a look at the least visible part...behind Addgene’s new search  Like millions of other websites, we built our new search engine atop Elasticsearch...

  15. Addgene: The First Twenty Years

    Type
    Blog Post
    ...theaters, the Razr was the hottest phone on the market, and two new websites, Facebook and Gmail, were ...

  16. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally high enough for this approach... guest blogger Didem Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik....

  17. The time and cost required to make a plasmid

    Type
    Blog Post
    ...reagents and consumables, especially for things like plates and gels, which either don’t scale or have limited...my ability to tell. But even from these rough estimates, it’s clear that community engagement with and...

  19. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...and isolated with antibiotic selection. The team tested this system in vitro and in vivo. They identified...fluorescent proteins into one bright particle that segregates into one cell during cell division. To achieve...

  20. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...(Japanese, German, and English) and even on 60 Minutes. We are blessed with a large community of scientists... employee pool and we get a lot of successful candidates from internal referrals. Our long reaching reputation...
Showing: 701 - 720 of 958 results