We narrowed to 729 results for: STO

... sterile sodium phosphate dibasic (NaH 2 PO 4 ) Store up to 1 month at 4 °C Adjust pH to 7.0 Autoclave...Gravitrap columns and gently pour off the ethanol storage buffer. Tightly attach LabMate PD10 Buffer Reservoirs...washing with 25 mL of Protein A/G binding buffer and store in 20% ethanol at 4 °C . Pro-Tip Columns may be ...Centrifuge column at 1000 x g for 2 min to remove storage solution. Place a mark on the side of the column...antibody to 1 mg/mL with PBS if needed. For long term storage, add sterile sodium azide to 1 mM. Option 2: Buffer... the NanoDrop Spectrophotometer. For long term storage, add sterile sodium azide to 1 mM....

...equipment back to room temperature, as prolonged storage in the cold room may damage it. Using a pipettor...quantification , which can be modified for RNA. Store your RNA sample(s) at -80 °C to prevent RNA degradation...sample, consider making smaller aliquots of it and storing those in -80°C. Option #2 - TRIzol® Protocol Homogenize...DNA quantification , which can be modified for RNA.Store your RNA sample(s) at -80 °C to prevent RNA degradation...sample, consider making smaller aliquots of it and storing those in -80°C. Solution D Protocol TRIzol® Protocol...

...into sterile tubes. Store the tubes at -80 °C. After thawing, the solution can be stored at 4 °C for up to... glutaGRO 11 mL of 200 mM L-alanyl-L-glutamine. Store at 4 °C. D1 + 0.1 M sorbitol (optional) : DMEM ...months, discard the tube and thaw a new working stock. 40% POLYETHYLENE GLYCOL (PEG) 8000 solution: Dissolve... solution may separate into phases. Aliquot and store at 4 °C. Cell Lysis Buffer : 50 mM Tris HCl, 150....5 Filter sterilize through a 0.22 μm membrane. Store at 4 °C. Considerations Before You Start The health...

...Prepare 25 ng/µL stocks of each sample. Samples can be used for ddPCR immediately or stored at -20 °C until...For the first virus dilution, add 160 µL of viral stock to 240 µL of media. When adding the virus to the...Dilute Media Volume Total Volume 2.5 160 µL viral stock 240 µL 400 µL 5 200 µL of 2.5-fold dilution 200 ... is an additional 10-fold dilution of the virus stock! Don’t forget to factor this into your calculations...transduce cells: Initial dilution factor of virus stock Dilution factor after additional 10-fold dilution...

...Transformation Plasmid Cloning Creating Bacterial Glycerol Stocks Restriction Digests Guides Browse our in-depth ...

...much virus as possible. Store at 4 °C for short term (2 weeks), or aliquot and store at -80 °C for long term...Sterilize by passing through a 0.22 μm filter and store at 4 °C. 1X PBS-MK buffer Dissolve 26.3 mg of MgCl...Sterilize by passing through a 0.22 μm filter and store at 4 °C. 0.1% Poloxamer 188 in PBS (A) Add 500 µL...clean fractions. Concentration and buffer exchange: Stocks of Poloxamer 188 Poloxamer 188, 10% solution A:...

... mM EDTA Store Solution I at 4°C Solution II - Denaturing Solution 0.2 N NaOH 1.0% SDS Store Solution ...acetate 23 mL glacial acetic acid 57 mL of dH 2 O Store Solution III at 4°C Grow 2 mL overnight cultures...containing your plasmid of interest. Add 1.5 mL of the stock culture to a 1.75 mL microfuge tube. Centrifuge ... room temperature before quantifying and using. Store DNA at 4°C. Tips and FAQ Plasmid purification kits...

...Bacterial Culture Introduction If you have a glycerol stock or stab culture of bacteria and need to purify plasmid...population (single colony) of bacteria from this stock. Using a single colony from a freshly streaked agar...Obtain the approrpriate bacterial stab or glycerol stock . Using a sterile loop, pipette tip or toothpick... of the stab culture or the top of the glycerol stock. Pro-Tip If you use a wire loop you can sterilize...

...viral stock will be higher titer, consider additional dilutions. Dilution Volume of Lentivirus Stock (μL...heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and lots of FBS...it is recommended that you titer from the frozen stock to account for any loss in titer associated with...

...Reagents TAE ( recipe here ) Agarose Ethidum bromide (stock concentration of 10 mg/mL) Gel loading buffer, New...Pro-Tip It is a good idea to microwave for 30-45 sec, stop and swirl, and then continue towards a boil. Keep...approximately 0.2-0.5 μg/mL (usually about 2-3 μl of lab stock solution per 100 mL gel). EtBr binds to the DNA ...sample is unloaded, push the pipettor to the second stop and carefully raise the pipette straight out of ...

... The above graph displays the stock concentration of Carbenicillin stock used (150 µL per plate). Please... concentration (100 mg/mL, 1000x) carbenicillin stock solution in sterile water (or other antibiotic) ...

...because bacteria are used as the means for both storing and replicating plasmids. Because of this, nearly...for transforming plasmid DNA for the purposes of storage and amplification. Higher efficiency cells are ... (containing the appropriate antibiotic ) from storage at 4°C and let warm up to room temperature and ...

...cells. LITE Two-hybrid system integrating the customizable TALE DNA-binding domain with cryptochrome 2 ...

...heat inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and lots of FBS...it is recommended that you titer from the frozen stock to account for any loss in titer associated with... to Plate (μL) Final Viral Dilution 1:10 100 of Stock Virus 900 150 1,350 1:100 1:100 100 of 1:10 900 ...

...posted sequence in the "View Sequence" link. Is your custom primer found within the Addgene sequencing results...require additional sequencing and need to design a custom primer, Addgene recommends using Addgene's sequencing...

... reaction, in which a single dNTP is included to stop the exonuclease function of the enzyme and shift...vector (shown in blue here). The T4 reaction may be stopped by heating to 75° for 20 minutes. Step 6: Anneal...

...enzymes Diagnostic Restriction Digest Protocol Storing and Handling Addgene Plasmids What to do after ...receive your plasmids from Addgene Instructions for Storing and Handling Plasmids Genomic Deletions with CRISPR...

...Watch the Video! Creating Bacterial Glycerol Stocks Store bacterial strains or plasmids for long term ...

...nM DAPI working solution: Prepare a 300 µM DAPI stock solution by diluting 2.1 µL of the 5 mg/mL DAPI ...working solution by diluting 5 µL of the 300 µM DAPI stock solution into 5 mL PBS. Protect from light. Procedure...

... heat-inactivated FBS and 5mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and FBS lots can...and have been sufficiently expanded, prepare cell stocks and/or harvest to test for protein expression. ...