Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 704 results for: des.1

Showing: 701 - 704 of 704 results

  1. DNA Quantification

    Type
    Protocol
    ... measure your samples, place 1-2µL of mini-prepped DNA onto the pedestal. Close the lid and click measure...Repeat for each sample. Notes: Keep in mind that despite the accuracy of the NanoDrop, if two consecutive...

  2. Water Bath Protocol

    Type
    Protocol
    ...quicker. Pro-Tip Set up the water bath 30 minutes to 1 hour before you need to use it to allow the temperature... of bacteria or fungi. There are disinfectants designed to be used in water baths with instructions on...bath on and set the appropriate temperature as described in your protocol. How to set the temperature will...Set up your water bath so that it will reach the desired temperature by the time you need it for your experiment...water bath to prevent evaporation and maintain the desired temperature. This also helps the water bath heat...water bath even if you are using disinfectants as described above in step 3. You will also need to maintain...

  3. Gibson Assembly Protocol

    Type
    Protocol
    ...ssDNA. (Rabe & Cepko, 2020). Incubate the mix for 1 hour at 50 °C or follow manufacturer's instructions... Procedure Design your plasmid and order primers (see figure to the right). When designing your plasmid...and enrich for correctly assembled plasmids by designing primers to split an antibiotic resistance gene...and yield. If there are significant amounts of undesired product, gel-purify DNA segments. Otherwise, PCR... High-Fidelity DNA Polymerase - incorporates nucleotides to “fill in” the gaps in the annealed DNA fragments... (<150 bp). Pro-Tip Please note that the way to design the “stitching” primers and the amounts of primers...window) . NEB has other resources, such as a primer design tool (Link opens in a new window) . Gibson Assembly...

  4. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...solution, add 2-2.5 volumes 95% or 100% ethanol and 1/10 volume of 3 M Na-acetate (pH 4.8). Invert the microfuge...Last Update: Feb. 8, 2018 Equipment Desktop microcentrifuge Desktop vortexer Vacuum (optional) Reagents... several μg/μL. The protocol below is meant to describe the general procedure for purifying plasmid DNA...
Showing: 701 - 704 of 704 results