We narrowed to 761 results for: Rest

1. Is this the right place for me? 8 tactics for choosing a lab

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   Blog Post

   ... aspects) applicable to industry if this path interests you  (e.g. human disease or models of disease,...

2. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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   Blog Post

   ...description to watch any sections you’re particularly interested in, but I’d recommend watching the whole livestream...

3. CRISPR 101: Off-Target Effects

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   ...making specific knock-out edits in a gene(s) of interest and has an average off-target frequency of 5%....

4. Viral Vectors 101: Optogenetic Tools

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   ...entire signaling pathways, but what if you’re interested in studying signaling pathways? Here’s where ...

5. Antibodies 101: Multiplex Immunofluorescence

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   ...quantification). But what if you’ve got several interesting protein targets and you want to know if they ...

6. Plasmids 101: Repressible Promoters

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   Blog Post

   ...parts, both of which must be present for a gene of interest to be expressed. These permit exquisite control...

7. Building Global Connections with the International Mentorship Program USA-EUROPE

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   Blog Post

   ...the non-profit IMFAHE, Inc. She is particularly interested in breast cancer and metastasis as well as teaching...

8. Antibodies 101: Antibody Engineering and Directed Evolution

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   Blog Post

   ...mutagenesis to refine antibody performance. If you are interested in learning more about antibody engineering technologies...

9. Deep Dive: Statistical Tests (Comparisons)

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   Blog Post

   ... available. Tailed t-tests If you are solely interested in whether or not two samples are statistically...

10. Typing CRISPR Systems

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    Blog Post

    ...CRISPR-Cas9 kicked off over a decade of frenzied interest in bacterial and archaeal immune systems. During...

11. CRISPR 101: Cytosine Transversion Editors

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    Blog Post

    ...bases. Thus, base editors have an activity window restricted to the stretch of ssDNA created by Cas9 binding...

12. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    Blog Post

    ...factors are directly injected into the organ of interest for in vivo transdifferentiation. Multiple cell...

13. Optogenetics + CRISPR, Using Light to Control Genome Editing

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    Blog Post

    ...respectively. The use of far-red light is of particular interest since blue light has limited applications in vivo...

14. CRISPR/Cas9 FAQs Answered!

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    Blog Post

    ...template, you could then: Confirm your HR by doing Restriction Fragment Length Polymorphism (RFLP) (see Figure...

15. DNA Quantification

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    Protocol

    ...Recovering Plasmid DNA from Bacterial Culture Restriction Digest of Plasmid DNA Background Information ...

16. Protocol - How to Perform Sequence Analysis

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    Protocol

    ...also like... How to Design a Primer Diagnostic Restriction Digest Introduction Sequence verification of ...

17. Water Bath Protocol

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    Protocol

    ...temperature, catalyze chemical reactions such as restriction digests needed in molecular biology, and thaw...

18. Fluorescence Titering Assay

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    Protocol

    ...with multiple integration events leading to underestimation of the true titer. Calculate the transduction...

19. Lentivirus Production

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    Protocol

    ..., and Transfer plasmid encoding your insert of interest Reagent Preparation DMEM Complete (also known ...

20. Using a Light Microscope Protocol

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    Protocol

    ... your field of view is centered on a region of interest. If needed, you can also use the fine focus knob...