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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 747 results for: UTY

Showing: 721 - 740 of 747 results

  1. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...gently mix. Notes: Make the 50% glycerol solution by diluting 100% glycerol in dH20. Snap top tubes are...5-6 times). Make sure that you see one uniform solution, and there are no layers present. Be sure to label...

  2. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...do you get better resolution of bands? A couple simple ways to increase the resolution (crispness) of your...agarose gel, including tips to help improve the resolution and separation of bands. Protocols...

  3. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...includes all of the precautions needed in BSL-1, along with additional precautions to prevent injuries...use 70% ethanol, quaternary ammonium, or bleach solutions. Ask your lab supervisor what is available and...

  4. Using a Light Microscope Protocol

    Type
    Protocol
    ...image and the resolution your microscope can achieve. You achieve the highest resolution when your image...magnification (the ability to make an image larger) and resolution (the ability to distinguish between two discrete...

  5. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...the bottom before placing it in the PCR machine. Diluting Primers Most people order primers from a company...concentration of each primer (10uM) by making a 1:10 dilution of the stock. For example, add 100µl of primer...cations such as Mg 2+ and Mn 2+ stabilize the buffer solution. These cations can also be used for PCR-mediated...

  6. Water Bath Protocol

    Type
    Protocol
    ...with water. You may use a 10% bleach solution or a 70% ethanol solution to wipe down the inside of the water...

  7. Centrifugation

    Type
    Protocol
    ...around the center (see image to right). Even distribution ensures that the centrifuge is balanced. Using...given as Relative Centrifugal Force (RCF) or Revolutions Per Minute (RPM), these are not the same units...

  8. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...D., & Kay, M. A. (2003). From virus evolution to vector revolution: use of naturally occurring serotypes...engineering and evolution of adeno-associated viral vectors for enhanced gene transfer . Pharmaceutical Research...enhanced function after intravenous administration, distributing widely throughout the organism to target large...viral preps . AAV retrograde (AAVrg) Directed evolution has also been employed to engineer an rAAV serotype...capsids, and capsids created through directed evolution. Created with BioRender.com. Common Uses of AAV...office can provide more information on your institution's best practices with regard to AAV-related research...vector integration . Current Opinion in Molecular Therapeutics, 11 (4), 442–447. PMID: 19649989 (Link opens...

  9. CRISPR Guide

    Type
    Guide
    ...close enough to cause a DSB. The nickase system is utilized in many CRISPR systems to achieve specific genetic... in nature, but have been created by directed evolution of the Escherichia coli TadA, a tRNA adenosine...window. These systems are well-suited to directed evolution applications. Examples of these base editing systems...activity. Knockout libraries often target 5′ constitutively expressed exons, while activation and repression...repression, many CRISPR epigenetic tools require constitutive expression of the dCas9 fusion and/or expression...CAPTURE system, developed by the Jian Xu lab , utilizes biotin tagging of dCas9 by fusing a biotin acceptor... Kim, D., Kim, S., & Kim, J. (2018). Directed evolution of CRISPR-Cas9 to increase its specificity. Nature...

  10. Promoters

    Type
    Guide
    ... factor 1 alpha CAG Constitutive Strong hybrid mammalian promoter PGK Constitutive Mammalian promoter ...Description T7 Constitutive but requires T7 RNA polymerase Promoter from T7 bacteriophage Sp6 Constitutive but requires...Research Promoter Expression Description CMV Constitutive Strong mammalian promoter from human cytomegalovirus...Inducible Tetracycline response element promoter U6 Constitutive Human U6 nuclear promoter for small RNA expression...polymerase Promoter from Sp6 bacteriophage lac Constitutive in the absense of lac repressor (lacI or lacIq...

  11. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...polyacrylamide gel electrophoresis alongside serial dilutions of a standard antibody of known concentration....labs use unpurified tissue culture supernatant routinely without any issues. Plot the mg/mL versus the ...

  12. Western Blot

    Type
    Protocol
    ...1 Reagent A to Reagent B dilution of the BCA assay. Prepare serial dilutions of the BSA standard that ...

  13. Cloning

    Type
    Guide
    ...recombine DNA? There are many methods that have been utilized over the years to move around pieces of DNA. Oftentimes...in the DNA. In restriction cloning, scientists utilize specific restriction enzymes to cut dsDNA of interest...reducing the amount of time required for cloning. To utilize this approach, the fragment of DNA that you would...pairs adenine (A) and thymine (T). TOPO® cloning utilizes the Taq polymerase which naturally leaves a single...traditional restriction enzyme cloning. Scientists have utilized this approach to create compatible custom overhangs... 100kb) compared to other cloning methods that utilize bacteria where it becomes progressively more difficult...

  14. Optogenetics Guide

    Type
    Guide
    ...for light-induced cAMP modulation. Utilizes BLUF (blue light utilizing FAD) domains. 441 Phytochromes BphP1...technologies developed for optogenetics research utilize light to detect, measure, and control molecular...expressed under the control of the vesicular gamma aminobutyric acid (GABA) transporter (VGAT) and ChR2 is thus...figure above, this light induced binding can be utilized to control the localization of a protein of interest...of neural activity, with single-spike temporal resolution. PLoS One. 2(3):e299. PMID 17375185 Kim B, Lin...

  15. Plan Your Experiment

    Type
    Guide
    ...your specific application. Knockout : Target constitutively expressed regions, 5’ exons, or an exon known...cell type. Figure 2: Different delivery systems utilized for CRISPR/Cas gene editing. This image summarizes... frequently use AAVs to develop CRISPR-based therapeutics. One limitation of AAV vectors is that the packaging...will ultimately depend on which CRISPR method you utilized. If your edit affects genome sequence, your edited...Chen, P. J., & Liu, D. R. (2022). Designing and executing prime editing experiments in mammalian cells. ...

  16. Lentiviral Vector Guide

    Type
    Guide
    ...followed by transduction is necessary to properly utilize lentiviral vectors. Some lentiviral plasmids (mostly...is still random and difficult to predict. In therapeutic applications, like chimeric antigen receptor ...particles. However, VSV-G is toxic under long-term, constitutive expression. In order to make stable cell lines...office can provide more information on your institution's best practices with regard to lentiviral-related...Third-generation plasmids use a hybrid 5' LTR with a constitutive promoter such as CMV or RSV. 3' LTR in cis Terminates...

  17. Chemogenetics Guide

    Type
    Guide
    ...vivo and the receptors exhibited high levels of constitutive activity, making them less than ideal. DREADDs...insensitivity to endogenous ligands, have low constitutive activity, and their activating ligands have ...This DREADD was shown to have a small amount of constitutive activity, and is not widely used. There is also...cross the blood-brain barrier in rats, and the utility of DREADDs in experiments in this species may be...

  18. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...particles. However, VSV-G is toxic under long-term, constitutive expression. In order to make stable cell lines...and provide stable integration of the intended therapeutic. While similar to lentiviral vectors, they offer...office can provide more information on your institution's best practices with regard to gamma-retroviral-related...Some newer plasmids use a hybrid 5' LTR with a constitutive promoter such as CMV or RSV. 3' LTR in cis Terminates...

  19. Molecular Biology Reference

    Type
    Guide
    ... DNA sequencing of the plasmid. Primers can be utilized to verify the sequence of the insert or other ...ampicillin. Preparing Antibiotics Create a stock solution of your antibiotic. Unless otherwise indicated...dH 2 0. Addgene recommends making 1000X stock solutions and storing aliquots at -20°C. To use, dilute ..., like Sanger, is based on DNA replication and utilizes modified fluorescently tagged nucleotides. During...

  20. Adenovirus Guide

    Type
    Guide
    ...response quickly, which has limited their use in therapeutic applications. However, this has also made them...office can provide more information on your institution's best practices with regard to AdV research. ...for outbreak pathogens . Human Vaccines & Immunotherapeutics, 13 (12), 3020–3032. https://doi.org/10.1080...
Showing: 721 - 740 of 747 results