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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 404 results for: pho;

Showing: 371 - 380 of 404 results

  1. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ... the phosphatase you choose. CIP (calf alkaline phosphatase) or SAP (shrimp alkaline phosphatase) are ...to use a single enzyme, but this will require phosphatase treatment of your recipient plasmid as well as...overhangs after digestion, you will need to use a phosphatase to prevent re-circularization of the recipient...treat your digested recipient plasmid with a phosphatase prior to the ligation step or prior to the gel...

  2. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis Introduction Restriction enzymes are naturally...and analyze the resulting fragments by gel electrophoresis . The pattern of the fragments on the gel can...to analyze a restriction digest. Equipment Electrophoresis chamber Pipetman Pipet tips Reagents Liquid...manufacturer's instructions) Gel loading dye Electrophoresis buffer Verifying Total Plasmid Size -OR- Insert... tricks see the Restriction Digest and Gel Electrophoresis pages....

  3. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ... the phosphatase you choose. CIP (calf alkaline phosphatase) or SAP (shrimp alkaline phosphatase) are ...overhangs after digestion, you will need to use a phosphatase to prevent re-circularization of the vector. ... treat your digested recipient vector with a phosphatase prior to the ligation step or prior to the gel...

  4. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...gel electrophoresis, which separates DNA by their length in base pairs. Following electrophoresis, you...Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis DNA Ligation Background Information Gel purification...Protocol: Gel Purification Follow the Agarose Gel Electrophoresis Protocol with the following amendments: Notes...

  5. Video Library

    Type
    Protocol
    ... Technology Protocol Agarose Gel Electrophoresis Gel electrophoresis is the standard laboratory procedure...visualization and purification. Agarose Gel Electrophoresis Protocol Purifying DNA from an Agarose Gel ...DNA fragments based on size. Following gel electrophoresis, you can cut DNA bands out of the agarose gel...

  6. General Transfection

    Type
    Protocol
    ...min. 25 mM chloroquine diphosphate Dissolve 0.129 g of chloroquine diphosphate salt into 10 mL of sterile...fresh DMEM complete containing 25 µM chloroquine diphosphate and incubate ~5 h For 10 mL of DMEM complete,...complete, add 10 µL of 25 mM chloroquine diphosphate. Dilute 18.9 µg of DNA into 500 µL of Opti-Pro SFM. Pro-...

  8. Pipetting Protocol

    Type
    Protocol
    ...Information Anatomy of a Pipette This front view photo of a P1000 pipette shows the plunger at the top,... pipette tip off of the pipette. This side view photo of a P1000 pipette shows the volume adjustment ring...ready to begin using the pipette. To the right is a photo of how you might hold a pipette. Without moving ...

  9. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...of each well at 450 nm on the spectrophotometer. Use the spectrophotometer to generate a standard curve...an automated ELISA plate washer. Equipment Spectrophotometer compatible with 96-well plates 1–10 µL single...

  10. Ligation Independent Cloning

    Type
    Protocol
    ...cloning scars"). A “stuffer” sequence allows for electrophoretic separation of linearized vector from the reaction...linearized vector from the reaction mixture by gel electrophoresis followed by gel purification . The cut vector...
Showing: 371 - 380 of 404 results