We narrowed to 799 results for: Clu

...visualize the DNA in your gel (this table is not inclusive of all gel stains):  Stain Pre or Post Run?...

...Other methods of generating a monoclonal cell line include single-cell sorting (Link opens in a new window... protocol will vary based on several factors, including the growth properties of the cell lines, the effects...cell pool by trypsinization and break up any cell clumps into individual cells by passing several times ...Wild-type A549 cells do not express Cas9 and are included as a control (WT). Vinculin is a loading control...

...Microbial rhodopsins are encoded by opsin genes, and include bacteriorhodopsins, halorhodopsins, and channelrhodopsins...

...of applications of the Enhanced Nano-Lanterns including monitoring multiple cellular events such as the...

...helped assemble a crack team of Addgenies that includes: Michelle, the Senior Scientist who coordinates...

... it includes labs that work with agents associated with diseases in healthy humans. BSL-2 includes all...goggles in addition to the BSL-1 requirements. Conclusion Although simple, following appropriate PPE protocol...

...have helped create several new imaging methods, including photoactivation-localization microscopy (PALM,...

...In this case, we will include the free nucleotide dGTP in the reaction (exclude all other nucleotides ...chew back" reaction, in which a single dNTP is included to stop the exonuclease function of the enzyme...

...used when the part to be inserted is too long to include on overlapping PCR primers (>60 bp) but too short...stitching” primers and the amounts of primers to include in the Gibson reaction are different than with ...window) sells DNA Assembly kits and master mixes, including NEBuilder HiFi (Link opens in a new window) and...

...methods. Some of the more common delivery methods include retrovirus [2], lentivirus [4], adenovirus [5],...

... purify plasmid DNA from a bacterial culture, including protocols, tips, and FAQ...the bacteria to lyse, releasing their contents, including plasmid DNA, into solution. Add a renaturing solution...step 6 to eliminate RNA contamination. This is included in the resuspension buffer of most kits. Reference...

...groups have made improvements to base editors, including: expanding targeting scope improving editing ...

... a certain position within the primer, one can include more than one nucleotide at that position termed...have complementary regions Note: If you will be including a restriction site at the 5’ end of your primer...

...Protocol for creating a glycerol stock, including additional tips for long-term storage....the appropriate temperature. Growth conditions, including copy number and growth temperature, can be found...

...Protocol for how to purify DNA from an agarose gel, including tips to help improve the resolution and separation...increase the resolution (crispness) of your DNA bands include: a) running the gel at a lower voltage for a longer...

...to consider when selecting sequencing primers, including: Is the primer unique? Make sure the primer only...your plasmid, please email [email protected] and include: The sequencing result The original trace file ...

... You need to do this because you don’t want to include the weight of the weighing boat or paper into your...paper in the biohazard solid waste container. Conclusion Appropriately weighing reagents is important ...

...A collection of Addgene's video content, including how-to screen captures, lab procedure protocols, and...some of the key strategies of aseptic technique, including personal protective equipment, setting up a clean...

... or recommend specific products or equipment. Inclusion of this information is solely for transparency... is both functioning as expected and specific, include a positive control sample that you know expresses...

...protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation of bands...increase the resolution (crispness) of your DNA bands include: a) running the gel at a lower voltage for a longer...