... it includes labs that work with agents associated with diseases in healthy humans. BSL-2 includes all...goggles in addition to the BSL-1 requirements. Conclusion Although simple, following appropriate PPE protocol...

...In this case, we will include the free nucleotide dGTP in the reaction (exclude all other nucleotides ...chew back" reaction, in which a single dNTP is included to stop the exonuclease function of the enzyme...

...have helped create several new imaging methods, including photoactivation-localization microscopy (PALM,...

...used when the part to be inserted is too long to include on overlapping PCR primers (>60 bp) but too short...stitching” primers and the amounts of primers to include in the Gibson reaction are different than with ...window) sells DNA Assembly kits and master mixes, including NEBuilder HiFi (Link opens in a new window) and...

...methods. Some of the more common delivery methods include retrovirus [2], lentivirus [4], adenovirus [5],...

... purify plasmid DNA from a bacterial culture, including protocols, tips, and FAQ...the bacteria to lyse, releasing their contents, including plasmid DNA, into solution. Add a renaturing solution...step 6 to eliminate RNA contamination. This is included in the resuspension buffer of most kits. Reference...

...groups have made improvements to base editors, including: expanding targeting scope improving editing ...

... a certain position within the primer, one can include more than one nucleotide at that position termed...have complementary regions Note: If you will be including a restriction site at the 5’ end of your primer...

...Protocol for creating a glycerol stock, including additional tips for long-term storage....the appropriate temperature. Growth conditions, including copy number and growth temperature, can be found...

...Protocol for how to purify DNA from an agarose gel, including tips to help improve the resolution and separation...increase the resolution (crispness) of your DNA bands include: a) running the gel at a lower voltage for a longer...

...to consider when selecting sequencing primers, including: Is the primer unique? Make sure the primer only...your plasmid, please email [email protected] and include: The sequencing result The original trace file ...

... You need to do this because you don’t want to include the weight of the weighing boat or paper into your...paper in the biohazard solid waste container. Conclusion Appropriately weighing reagents is important ...

...A collection of Addgene's video content, including how-to screen captures, lab procedure protocols, and...some of the key strategies of aseptic technique, including personal protective equipment, setting up a clean...

... or recommend specific products or equipment. Inclusion of this information is solely for transparency... is both functioning as expected and specific, include a positive control sample that you know expresses...

...protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation of bands...increase the resolution (crispness) of your DNA bands include: a) running the gel at a lower voltage for a longer...

...cloning by restriction enzyme digest (subcloning), including design and experimental procedures....Design (Choosing enzymes) Many DNA analysis tools, including Addgene’s Sequence Analyzer , allow you to identify...

... or recommend specific products or equipment. Inclusion of this information is solely for transparency...handling, use proper personal protective equipment including laboratory coat, laboratory goggles, and gloves...

... or recommend specific products or equipment. Inclusion of this information is solely for transparency... both functioning as expected and is specific, include a positive control sample that you know expresses...

... or recommend specific products or equipment. Inclusion of this information is solely for transparency... of the standard curve you will need to either include higher concentrations in the standard curve or ...

...without a kit. The general steps of this protocol include homogenization/lysis of cells or tissues, extraction...For the precipitation step, two options are also included: using Isopropanol or Lithium Chloride. This protocol...