We narrowed to 802 results for: Clu

1. Technique: Probe Phage Genomes for Host Binding Proteins

   Type

   Blog Post

   ...refer to the paper for more details on the assay, including examples of RBPs successfully identified using...

2. Recombinase-based State Machines Enable Order-dependent Logic in vivo

   Type

   Blog Post

   ...recombinase-specific co-factors (which should be excluded from the system), these reactions are irreversible...

3. Generating Mouse Models Using CRISPR/Cas9

   Type

   Blog Post

   ...targeting, which is limited to a few strains, including 129 and C57BL/6, for which we have germline competent...

4. Plasmids 101: Restriction Cloning

   Type

   Blog Post

   ...visualize the DNA in your gel (this table is not inclusive of all gel stains):  Stain Pre or Post Run?...

5. Isolating a Monoclonal Cell Population by Limiting Dilution

   Type

   Protocol

   ...Other methods of generating a monoclonal cell line include single-cell sorting (Link opens in a new window... protocol will vary based on several factors, including the growth properties of the cell lines, the effects...cell pool by trypsinization and break up any cell clumps into individual cells by passing several times ...Wild-type A549 cells do not express Cas9 and are included as a control (WT). Vinculin is a loading control...

6. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

   Type

   Blog Post

   ...Microbial rhodopsins are encoded by opsin genes, and include bacteriorhodopsins, halorhodopsins, and channelrhodopsins...

7. Transferable Skills Guide: Teamwork

   Type

   Blog Post

   ...helped assemble a crack team of Addgenies that includes: Michelle, the Senior Scientist who coordinates...

8. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

   Type

   Protocol

   ... it includes labs that work with agents associated with diseases in healthy humans. BSL-2 includes all...goggles in addition to the BSL-1 requirements. Conclusion Although simple, following appropriate PPE protocol...

9. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

   Type

   Blog Post

   ...have helped create several new imaging methods, including photoactivation-localization microscopy (PALM,...

10. Ligation Independent Cloning

    Type

    Protocol

    ...In this case, we will include the free nucleotide dGTP in the reaction (exclude all other nucleotides ...chew back" reaction, in which a single dNTP is included to stop the exonuclease function of the enzyme...

11. Gibson Assembly Protocol

    Type

    Protocol

    ...used when the part to be inserted is too long to include on overlapping PCR primers (>60 bp) but too short...stitching” primers and the amounts of primers to include in the Gibson reaction are different than with ...window) sells DNA Assembly kits and master mixes, including NEBuilder HiFi (Link opens in a new window) and...

12. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    ...methods. Some of the more common delivery methods include retrovirus [2], lentivirus [4], adenovirus [5],...

13. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type

    Protocol

    ... purify plasmid DNA from a bacterial culture, including protocols, tips, and FAQ...the bacteria to lyse, releasing their contents, including plasmid DNA, into solution. Add a renaturing solution...step 6 to eliminate RNA contamination. This is included in the resuspension buffer of most kits. Reference...

14. CRISPR 101: Cytosine and Adenine Base Editors

    Type

    Blog Post

    ...groups have made improvements to base editors, including: expanding targeting scope improving editing ...

15. Protocol - How to Design Primers

    Type

    Protocol

    ... a certain position within the primer, one can include more than one nucleotide at that position termed...have complementary regions Note: If you will be including a restriction site at the 5’ end of your primer...

16. Protocol - How to Create a Bacterial Glycerol Stock

    Type

    Protocol

    ...Protocol for creating a glycerol stock, including additional tips for long-term storage....the appropriate temperature. Growth conditions, including copy number and growth temperature, can be found...

17. Protocol - How to Purify DNA from an Agarose Gel

    Type

    Protocol

    ...Protocol for how to purify DNA from an agarose gel, including tips to help improve the resolution and separation...increase the resolution (crispness) of your DNA bands include: a) running the gel at a lower voltage for a longer...

18. Protocol - How to Perform Sequence Analysis

    Type

    Protocol

    ...to consider when selecting sequencing primers, including: Is the primer unique? Make sure the primer only...your plasmid, please email [email protected] and include: The sequencing result The original trace file ...

19. Weighing Reagents Protocol

    Type

    Protocol

    ... You need to do this because you don’t want to include the weight of the weighing boat or paper into your...paper in the biohazard solid waste container. Conclusion Appropriately weighing reagents is important ...

20. Video Library

    Type

    Protocol

    ...A collection of Addgene's video content, including how-to screen captures, lab procedure protocols, and...some of the key strategies of aseptic technique, including personal protective equipment, setting up a clean...