We narrowed to 771 results for: VARS
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TypeBlog Post... quickly learn, and apply their knowledge to a variety of problems. In this post, I’ll share what I’ve...
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Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...brain region of interest could be low or highly variable without the behavioral test. Another readout may... -
CRISPR 101: Validating Your Genome Edit
TypeBlog Post...Pinello L. CRISPRitz: rapid, high-throughput and variant-aware in silico off-target site identification ... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...basic approaches to conflict resolution based on various levels of assertiveness and cooperation: avoiding... -
Plasmids 101: Biotinylation
TypeBlog Post...chemicals (biotinylation reagents). There are a variety of biotinylation reagents available that differ... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post... avoid scattering intellectual property across various software providers used to supplement their paper... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post...produce and distribute antibodies validate them in a variety of applications, including IF (or IHC or ICC), ... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...the Addgene Blog Learn about High Fidelity Cas9 Variants How to Design Your gRNA Delivering CRISPR Ribonucleoproteins... -
March for Science
TypeBlog Post...her PhD from the Systems Biology department at Harvard University. She is a scientist with a passion for... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post... temperature and length of induction, which will vary between different proteins. Beyond these standard... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...diseases, the mutational landscape is broad and varied, necessitating the development of many distinct... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...equal. Some work better than others. There are a variety of resources online to guide you in gRNA design... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...being used in any genetic background, but require variable amounts of experimental manipulation and screening... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...These synthetic RNAs can also be purchased from various other suppliers with a typical turnaround time ... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects include investigating... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post... their function. Their migration is induced by various types of interactions with their environment. These... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...genetically-encoded FRET donors (Fig. 1). Scores of FP variants have been created, thereby enabling a broad spectrum... -
Optogenetics Guide
TypeGuide... its variants allow for inhibition of neurons using blue-green light. Mac Variants. Mac variants have ...Inhibitory (Hyperpolarizing) ChR Variants. Alternatively, ChR variants that inhibit neurons have been created... these variants result in the hyperpolarization of neurons. Examples of anion channel variants from other...thus inhibiting function of the neuron. NpHR Variants. Variants have been engineered with enhancements such... triggered by green-yellow light. Arch Variants. Arch variants have been developed with the following ...plasmids . Glossary of Microbial Opsin Variants Opsin Type Variant Description Peak Response Spectra (nm...Phobos Blue-shifted iC++ variant 467 Aurora Red-shifted anion-selective ReaChR variant 517 Halorhodopsins Jaws... -
CRISPR Guide
TypeGuide...binding) SpCas9 VRER variant 3' NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9...LbCpf1) 5' TTTV AsCpf1 RR variant 5' TYCV LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter...out target genes in various cell types and organisms, but modifications to various Cas enzymes have extended...available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements, and...Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant 3' ...understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started...use, and there are several to choose from. Many variations of Cas9 exist, along with other Cas enzymes like... -
Antibody Guide
TypeGuide...Single-chain fragment variables (scFvs) contain only the variable heavy chain and variable light chain regions... way to produce antibodies, with the highest variability in antibodies produced. Cells - Antibodies can...cells). The genetic reorganization that creates variety in the antibody allowing for their extreme specificity...large amounts of a single antibody with little variation. Recombinant plasmids - Antibodies can be produced...the plasmid. This produces the least amount of variation between antibodies, allows for the antibody’s ...easily confirmed via sequencing, and enables the variable region and constant regions of the antibody to.... The tips of the Fab fragments contain highly variable regions, shown in light blue and light orange,...