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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,052 results for: FER

Showing: 761 - 780 of 1052 results

  1. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...overexpressed from a plasmid but overexpression may interfere with the system. Another method for studying protein...whether or not the tags influence behavior. A conference and a breakthrough! During my graduate studies...you can contact her at [email protected]. References 1. Dieterich, Daniela C., et al. "In situ visualization...

  2. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post
    ...newly independent faculty member studying sex differences in thoracic aortic disease,” Prakash says. In..., the lab hopes to capture the signalosome of different receptors upon stimulation. “To study cellular... Jennifer Tsang...

  3. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post
    ...dTomato) that promote interactions between the FPs interfere with native interactions, preventing proper localization...papers should be used with caution, since the difference between studies is substantial (e.g, the poor...conclude, we recommend that you try a couple of different fluorescent proteins when protein tagging is the...

  4. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post
    ...experiment moving. Happy editing! References and Resources References Hendel, A., Bak, R., Clark, J. et...used in CRISPR-Cas9 experiments – the “gRNA” – suffers from the same issues as your experimental RNA. ...

  5. Antibodies 101: Affinity Reagents

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    Blog Post
    ...bispecific, meaning that each scFv can target a different antigen; binding of each can be either simultaneous... a scientist looking for that perfect fit!  References and Resources Additional resources on the Addgene...AddgeneBrowse our recombinant antibody plasmid collection References Grumezescu, A. M. (2018). Drug targeting and ...

  6. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    Blog Post
    ...mutations). Although the precise mutations were different than those found in eSpCas9, the effect on specificity...and/or therapeutic settings. Will combining the different mutations found in each of these great tools further...continued improvements in genome engineering! References 1. Slaymaker, Ian M., et al. "Rationally engineered...

  7. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post
    ...replicates would have different labels - i.e. they would stochastically arise from different cells throughout...development of combination therapies.         References 1. Hyo-eun, C. Bhang, et al. "Studying clonal...

  8. Plasmids 101: Cre-lox

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    Blog Post
    ...because it “causes recombination” (although later referred to as the “cyclization recombinase”), is a 38 ...same type, but are not cross-compatible. Using different lox site variants allows Cre to catalyze more ...cre-dependent techniques?  Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination...

  9. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post
    ...methods flood the cell with protein, which can both interfere with a cell’s normal function and result in a ...portal. The plasmids should be functional in many different human cell types, and while most of the genes ...Allen Institute website at alleninstitute.org. References 1. Roberts, Brock, et al. "Systematic gene tagging...

  10. CRISPR in the Clinic

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    Blog Post
    ...updated in 2023 by Susanna Stroik.  References and Resources References Yuxuan Wu et al. "Correction of a...frequency isn’t high enough to make a physiological difference, then the treatment will not work in a meaningful...

  11. High Complexity Golden Gate Assembly with NEB’s DAD

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    Blog Post
    ...base or 4-base overhangs, and with a variety of different enzymes and cycling conditions (see Table 1). ... cycling 3-base  PaqCl®, 1x T4 DNA Ligase Buffer 37-16 °C cycling 4-base PaqCl, 1x NEBridge... and don’t forget to ask DAD for some help! References Potapov, V., Ong, J. L., Kucera, R. B., Langhorst...

  12. Viral Vectors 101: Virus Safety

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    Blog Post
    ...dividing up the essential components of a virus into different plasmids. For a retroviral system this looks like... like the genetic cargo in one plasmid (the transfer plasmid), the envelope (Env) gene on a separate plasmid...Env, Gag, and Pol genes are not encoded in the transfer plasmid, the component that physically integrates...

  13. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    Blog Post
    ...their mutations corrected, and the T-cells were transferred back into the infants. Almost all patients have...population. These pre-existing antibodies can interfere with virus-cell interactions and seriously hinder... cure research and science communication.     References 1. Gaspar, H. B., S. Howe, and A. J. Thrasher...

  14. Hot Plasmids: Winter 2025

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    Blog Post
    ... tomato plants that inherited an edited gene to confer resistance to the herbicide chlorsulfuron (Homo...want a negative-going GEVI that can be used in different illumination regimes with fast activation kinetics... a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically binds to active...

  15. Magnetic Control of Proteins: More than a Dream

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    Blog Post
    ...Find the MagLOV plasmid here!   References and resources References Duret, G., Polali, S., Anderson,...just be an artifact and eliminate sources of interference, but the result persisted. (Even better, multiple...

  16. Light Sheet Fluorescence Microscopy

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    Blog Post
    ...spinal tract, CST, spinal cord and cranial nerve afferents labelled with a AAV8 expressing eGFP under the...sample and captured the reflected light using a different objective lens oriented perpendicular to the plane...Find plasmids from the Tsoulfas lab here.     References: H. Siedentopf, and R,. Zsigmondy.  (1902) Ann...

  17. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ... lines with compound autosomes are completely infertile when mated to wild fruit flies6. It's also worth...information about gene drives can be found in the referenced publications. Thank you to our guest blogger...information, visit www.sculptingevolution.org. References: Burt, A. Site-specific selfish genes as tools...

  18. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post
    ...explains it, her work has always been focused on gene transfer, from transgenes to plants, chloroplast to the...anymore. The kit contains sequences for several different fluorescent proteins. Visualizing fluorescence...for those who go to work in cancer biology.  References: A Golden Gate Modular Cloning Toolbox for Plants...

  19. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post
    ...the physical DNA samples, for when that's the preferred mode of receiving parts." "We have found an amazing...functions, which we know and love. Boolean is in reference to George Boole who in 1854 was thinking about...tell us readers, what do you want to make?   References: Jerome Bonnet et al. "Amplifying genetic logic...

  20. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    Blog Post
    ...journal editorials, science social media, and conference workshops for years. That is, presumably, why...these fields, and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth, and Ed Boyden crop up...scientist who enjoys learning a little bit about many different things (part of the reason Addgene has been such...
Showing: 761 - 780 of 1052 results