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We narrowed to 972 results for: REP

Showing: 761 - 780 of 972 results
  1. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...Thesis (who has also blogged for us) offers a free report on her website called “Secrets to Success in Graduate...development to mentoring for women and under-represented minorities. Her online content is of great value...
  2. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...lot to lot specificity and tend towards more reproducible results. Since polyclonal antibodies contain... reactive ones. And make sure to check for any reported cross-reactivity when planning your experiment...
  3. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...problems with transgenic mice that can impact reproduction include embryonic lethality, a short lifespan... to mouse modeling now complete, you should be prepared to plan an experimental strategy that uses genetically...
  4. Common Injection Routes in Mice

    Type
    Blog Post
    ...finally, to expert.   Figure 1. Visual representation of common injection routes in mice. Image created...events. Intradermal injections require a bit of extra prep, as the injection site area must be shaved first...
  5. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...have been around since 2000 when Terskikh et al. reported the production of dsRed E5. This timer predictably...fluorescent timers that change color from blue to red report on cellular trafficking." Nature chemical biology...
  6. PhD Applications After COVID

    Type
    Blog Post
    ...transcripts if requested Thank them, and eagerly await a reply. It’s a good idea to email around three professors...about your research goals and education, too! Prepping for the interview Many universities offer free...
  7. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...cell types, gene editing using homology directed repair (HDR), and generating large genomic deletions. ...delivery. You’ve decided to use RNPs, now what? Preparing the Cas9-gRNA ribonucleoprotein The first step...
  8. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...club? Where will the journal club take place? Preparing for the first meeting There are many important...include the paper, any supplemental data, software repositories, or other information needed by someone to critically...
  9. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...of interest and an inactivated fluorescent RNA reporter. If the target sequence is present in the pool...activity of Cas13a becomes activated and the RNA reporter will be cleaved resulting in activation of the...
  10. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...targets can be done largely in vitro; Easy and reproducible commercial synthesis (those of us who have used...highly customizable in terms of how they actually “report” as biosensors. Making aptamers glow The flexibility...
  11. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ... abundance relative to cognate tRNA abundance Repetitive sequences Restriction sites Sequences prone to...isoacceptors induced by amino‐acid starvation." EMBO reports 6.2 (2005): 151-157. PubMed PMID: 15678157. PubMed...
Showing: 761 - 780 of 972 results