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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 960 results for: REP

Showing: 761 - 780 of 960 results

  2. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...cell types, gene editing using homology directed repair (HDR), and generating large genomic deletions. ...delivery. You’ve decided to use RNPs, now what? Preparing the Cas9-gRNA ribonucleoprotein The first step...

  5. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...club? Where will the journal club take place? Preparing for the first meeting There are many important...include the paper, any supplemental data, software repositories, or other information needed by someone to critically...

  6. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ... abundance relative to cognate tRNA abundance Repetitive sequences Restriction sites Sequences prone to...isoacceptors induced by amino‐acid starvation." EMBO reports 6.2 (2005): 151-157. PubMed PMID: 15678157. PubMed...

  8. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...of interest and an inactivated fluorescent RNA reporter. If the target sequence is present in the pool...activity of Cas13a becomes activated and the RNA reporter will be cleaved resulting in activation of the...

  9. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...targets can be done largely in vitro; Easy and reproducible commercial synthesis (those of us who have used...highly customizable in terms of how they actually “report” as biosensors. Making aptamers glow The flexibility...

  10. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...and triggering release of the stress hormone norepinephrine to promote vigilance. A recent study investigated...those neurons are chemogenetically activated, norepinephrine release causes a freezing response, a form ...

  11. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...package. There are three steps to chalk talk preparation: Define your research vision. Organize your ...proposal or a 10-year research plan. I suggest preparing for both types. Your research vision is your approach...

  12. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...space where you can address the issue head on. Prepare for this time as if it were a meeting. Gather the...change with them. If you can, set aside time to prepare for future challenges. This doesn’t have to be ...

  14. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...becomes available. For routine infections, like strep throat, testing for antibiotic resistance isn’t ...Tackling drug-resistant infections globally: final report and recommendations." London: Wellcome Trust & ...

  15. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ... Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell 159:647–661 . https://doi.org...on Addgene.org Find Cas9 Activators Find Cas9 Repressors Catch Up on Your CRISPR Background with Our CRISPR...

  16. Gibson Assembly Protocol

    Type
    Protocol
    ... antibiotic gene. This trick can also enable replacement of "inverse PCR" reactions with a two-part Gibson...

  17. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...interest with an additional protein module that can report on location. This approach enables the study of...9759496.  3. Buckley, Anthony M., et al. "LOV-based reporters for fluorescence imaging." Current opinion in ...

  18. Five Popular Model Organisms

    Type
    Blog Post
    ... giving birth - of about 10 weeks. Adult mice reproduce as often as every three weeks so scientists can...important gene co‑expression modules." Biomedical Reports 7.2 (2017): 153-158. PubMed PMID: 28804628. PubMed...

  19. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...able to ask follow up questions, or ask people to rephrase questions was key. I had also hoped that just ... 5: The mobile monitor and teleprompter being repurposed for a different shoot to create the How to Deposit...

  20. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...risks associated with old equipment, like service repairs, labour costs, and loss of time, can exceed the...community is the ultimate value proposition. By preparing a budget before hand, you’re saving the institute...
Showing: 761 - 780 of 960 results