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...monkeys it actually targets neurons, and has 45-fold higher expression levels compared to AAV9. You can now...AAV-CAG-GFP packaged in AAV9-X1.1!  We also have eight new AAV-PHP.eB vectors from the Jonathan Ting lab...

...proteins from E. coli is generally more straightforward and of a higher purity than mammalian purifications...

...mapping and design (Figure 1). Benchling: While you might think of Benchling as an electronic lab notebook...Design (DAD): This tool designs overhang sets for high complexity Golden Gate Reactions Cloning ligation...

...growing open repository of CRISPR screen data that might be able to help you out. What is the Open Repository... cross-interrogation of different datasets that might otherwise be hard to identify. Figure 2: ...

...leaders in the field of microbiology with multiple high-profile publications. My classmates consisted of...scientific manuscript is not prose; it is to be straight to the point. 3. Engage the reader Scientists ...

...celebrate our depositing PI’s who have the most highly requested plasmids. Currently, there are roughbly... highly requested plasmids...

... I settled on using glass slides. With just the right amount of agarose, I could pour a very thin gel ...surface tension.  It's a bit tricky to pour the right amount so the agarose doesn’t pour over the sides...

...period. While there are many aspects to choosing the right labs (see my webinar (updated link from 2020) on...Mentoring" Don’t ignore warning signs If lab members, neighbors or alumni give you information about the PI that...

... fused to mature fluorophores, and can bind and light up epitope tagged proteins in vivo. These probes...sites within the variable portions of heavy and light chains. Proceedings of the National Academy of Sciences...

...how aggressive my sentences might sound. What helps me in keeping the “right tone” is to address the comments...

...other reagents every day, and this crisis has highlighted the importance of access to quality materials...

...We hypothesized that this double stranding step might not actually be necessary. Since the AAV genome ...following DNA extraction, the [+] and [-] strands might naturally associate forming a double-stranded species...

..., is species compatible, and doesn’t come with a high background can be a huge challenge. Epitope tags...amino acids. FLAG tag has been used with moderate to high success rates across all applications. You can find...

...(Neuroscience AntiBody Open Resource). We are delighted to be partnered with Dr. James Trimmer of the ...this grant. Dr. Trimmer has created thousands of high-quality antibodies/nanobodies for neuroscience research...

...for similarly controlling neuronal activity with light will be available soon. We’re also distributing ...controls to determine if your AAVs are getting to the right cells using your particular delivery technique and...

...protocol takes about 2-3 weeks but requires less than eight hours worth of hands-on time. Creating a modular... the SEC-based system is both simple to use and highly efficient for C. elegans genome modification. ...

...makes NEBuilder HiFi so accurate (apart from the high-fidelity polymerase) is the ability to remove, reset...covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc...

... to address the effects of genetic variants in a high throughput manner. She helped develop a method that...shape and metabolism of leaves can be changed by light and temperature and leaf autofluorescence can interfere...

...). They saw that these AAV particles come in a slightly different shape. These AAV particles are often...purified AAV vectors. They found that they have a higher transduction efficiency, i.e. the same number of...

...focus on discussion and brainstorming Though, we might be forced to work from home and/or be in the lab...experience more interactive. The button is on the right of the Share Screen control panel on the top of ...