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We narrowed to 130 results for: primer

Showing: 31 - 40 of 130 results

  1. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...protothropic cassette can be amplified with the following primers: FW 5'–GGTGACGGTGCTGGTTTA–3' RV 5'–TCGATGAATTCGAGCTCG...

  2. Viral Production at Addgene

    Type
    Blog Post
    ...are titered by droplet digital PCR (ddPCR) using primers and probes targeting the ITR elements and an internal...incubated for 72 hours, and genomic DNA is extracted. Primers and probes targeting integrated copies of the lentiviral...the final viral preparation undergoes PCR with primers targeting the transfer plasmid used in the transfection...

  3. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...selectable markers for use in other cell types. Primer Binding Site A short single-stranded DNA sequence...initiation point for PCR amplification or sequencing. Primers can be exploited for sequence verification of plasmids...

  4. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...Electrophoresis Bacterial Transformation How to Design a Primer Creating Bacterial Glycerol Stocks Inoculating ... Videos What is a Plasmid? How to Design Primers for PCR Agarose Gel Electrophoresis Inoculating...Analysis We also introduced guides — practical primers to help users get started with tools like CRISPR...

  6. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...cloning: 1. Create Your PCR Product: Design standard primers (no need to add unique restriction sites on the...Pro tips Do not add 5’ phosphates to your PCR primers; you need that free hydroxyl group! You may want...

  7. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...unique barcoded Illumina-M13F bridging primer. These bridging primers are amplified and used to generate ... Type IIS restriction enzymes and standardized primers to clone your gene of interest into both sequencing...

  8. 5 Great Apps for Lab Life

    Type
    Blog Post
    ... great if you’re adding a restriction site to a primer, you only need to skim the alphabet on the side...helps you set up an optimal PCR depending on the primer sequence and the NEB polymerase used. Overall, ...

  9. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...by homology to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template...

  10. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ... that much different from conventional duplexed primer probe assays often used in real time PCR. These...NHEJ. These assays include a forward and reverse primer to amplify the target locus, as well as a reference...
Showing: 31 - 40 of 130 results