Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 642 results for: ato

Showing: 91 - 120 of 642 results

  1. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...include the laboratory personnel, the serum, the cell culture media, water baths, incubators, etc. Contamination... in a single incubator since it increases the risk of cross-contamination. CO2 incubators should be cleaned...Contamination! Tissue culture media often have indicators included in the media that turns yellow from ...proliferating shortly. Once they are present in the laboratory environment, they are difficult to eradicate....tighten the flask cap before placing them in the incubators. This will also solve the problem of contamination...airflow and the lab facility periodically. The CO2 incubators, used for maintaining cell lines, must not be...

  2. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...voltage indicator Article contributed by Shreya Vedantam Genetically encoded voltage indicators (GEVIs)...fluorescent optics. Existing red fluorescent voltage indicators, however, fall short compared to GFP-based sensors... sensors and blue-light-activated optogenetic actuators.  To overcome this overlap, Yiyang Gong’s lab ...linker between them. The Ace-mScarlet voltage indicator works like many FRET-opsin GEVIs. With an increase...channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids at Addgene! Beck et al., Sci Report 2019...cyanobacteria. This kit contains 96 parts including terminators and promoters, CRISPRi parts, selectable markers...

  3. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...tag neurons activated by opioid withdrawal with tdTomato and assessed cFos expression. She will explore...periaqueductal gray (PAG), with red neurons expressing tdTomato and green neurons expressing cFos. Image from ...painful stimuli are processed by a network of excitatory and inhibitory neurons whose function and connectivity...where she engineers genetically encoded voltage indicators. Tian developed a video-based high-throughput...archaerhodopsin‑derived genetically encoded voltage indicator which had improved performance in live mouse brains... Currently, she is using the improved voltage indicator coming from the screen to understand mouse cortical...Tian. Figure 5: Citrine-tagged voltage indicator expressed in mouse cortex. Image from He Tian....

  4. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...and helper T (Th) cells. These cells produce inflammatory cytokines - interleukin (IL)-12 and interferon... eliminate early stage cancer cells, chronic inflammatory responses can augment tumor growth. Indeed, ... to precede cancer development, for example, inflammatory bowel disease is associated with the onset of... 1 (CSF-1). Additionally, tumor cells recruit regulatory T cells (T-regs) into their microenvironment ...TGFβ is also known to change CD4+ Th cells into regulatory cells to suppress function. Creating Tolerance...cell-surface proteome to inhibit expression of co-stimulatory molecules needed for T cell effector function...identities based on their level of progression, anatomical location, characteristic (solid localized tumors...

  5. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...crosslinking agent, a signaling ion, and an allosteric modulator. Because of its importance, scientists have developed... a calcium sensor Genetically encoded calcium indicators (GECIs) are fluorescent proteins linked to calcium...in living cells and organisms. Unlike chemical indicators, these DNA constructs can be delivered to intact...:509-18. Inoue M. Genetically encoded calcium indicators to probe complex brain circuit dynamics in vivo...Kanemaru K, Iino M. Genetically encoded fluorescent indicators for organellar calcium imaging. Biophysical journal...neuronal activity with genetically encoded calcium indicators. Cold Spring Harbor Protocols. 2012 Jun 1;2012...signaling using genetically targetable fluorescent indicators. Nature protocols. 2006 Aug;1(3):1057-65. Resources...

  6. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...following: the elevator pitch, talks, the poster session, networking, and social media. The elevator pitch Before... Before the conference Practice your “elevator pitch,” the 30 - 60 second blurb you will use to introduce...work you are familiar with. You can tweak your elevator pitch slightly if you know a bit more about the...or talk. After the conference After using your elevator pitch several times at the conference, you might...might notice some trends. Were there parts of your elevator pitch where the other person seemed confused, ...questions. If you or your lab identifies a potential collaborator from the meeting, reach out to them, pointing...

  7. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...development programs for bioscience educators. Using these hands-on tools, educators introduce their students to...a backbone structure of a protein, based on its atomic coordinates. These wire models were useful in that...necessary to download a pdb file of the structure’s atomic coordinates, and “open” these structures with molecular...dangerous. Subscribe to CRISPR Blog Posts How do educators and students use molecular models?   We created...something tangible. Under the guidance of a trained educator, these instances of focused attention become magical... their labs. Soon after we began working with educators in our Center for BioMolecular Modeling, we spun...

  8. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...The first is the study of non-coding regulatory elements. Regulatory DNA function is typically inferred ...bloggers! Daniel Bauer is a pediatric hematologist and principal investigator at Boston Children’s Hospital and...interested in exploring the function of non-coding regulatory elements in their native chromatin context. An...PhD student at Harvard Medical School in the laboratories of Stuart Orkin and Daniel Bauer. He received...

  9. Synthetic Biology - Networks and Gene Regulation

    Type
    Collection
    ...Examples Include: promoters and terminators repressors and activators logic gates Networks and Gene Regulation...includes both naturally ocurring and synthetic regulatory elements, pre-assembled genetic circuits, and...

  10. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...employing optogenetic regulators to initiate the event being monitored. Optogenetic regulators respond to specific...initiation wavelengths, excitation of the optogenetic regulator leads to spurious activation of the FRET sensor...interested in using photoactivatable, optogenetic accuators to depolarize or hyperpolarize membranes. Traditional...wavelengths needed would also activate the optogenetic accuators. The researchers took a FRET reporter and adapted...biosensors that can even be paired with optogenetic regulators. Many of these tools are available from Addgene...2017) Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging...

  11. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...upon a moratorium until another meeting, three branches – a private funder, government regulators, and ...there was a generally agreed-upon international moratorium on utilizing CRISPR/Cas9 in humans. This topic...mtDNA transfer options being investigated; A UK regulatory body tasked two separate non-governmental groups...technology to research centers engaged in long-term, mandatory follow-ups and observational studies of the technique...stop punting decisions on scientific, ethical, regulatory, and general public consensus to the next meeting...

  12. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ...lipoproteins, shuttles lipoproteins through the circulatory systems, but can also lead to the formation of...as both a powerful tool for generating complex regulatory systems and further the study of natural σ54-... PMID: 31451697. Genetically encoded voltage indicators for use in worms Article contributed by Brook...Brook Pyhtila Genetically encoded voltage indicators (GEVIs) change fluorescence in real time when neurons... excitation light intensities have made these indicators difficult to work with.  The Gottschalk lab has...microbial rhodopsin GEVIs in excitable cells of the nematode Caenorhabditis elegans. They have found several...

  13. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...site-specific recombinases, regulatory elements, developmental regulators, and lots of viral components...test many different combinations of promoters, terminators, solubility and purification tags, connectors... examine the effects of different promoters, terminators, enhancers, and cross-cassette interactions in...Standard and New E. Coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology... Signaling in CAR T Cells. Cold Spring Harbor Laboratory, 17 Feb. 2024. Crossref, https://doi.org/10.1101...Emerging CRISPR Technologies. Cold Spring Harbor Laboratory, 27 Oct. 2023. Crossref, https://doi.org/10.1016...

  14. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...environmental modulation of hepatocellular cancer cell growth." Hepatology 54.4 (2011): 1237-1248. PubMed... was contributed by Bárbara Pinho, a science communicator at the Portuguese science museum "Fábrica Centro...myofibroblasts, neuroendocrine, adipose, immune, inflammatory, angiogenic vascular, endothelial, and stromal...seems to attack us by releasing mitogenic growth mediators and improving cancer cells proliferation.  How... Pinho! Bárbara Pinho is currently a science communicator at the Portuguese science museum "Fábrica Centro...

  15. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Dorus Gadella 37351 pQC membrane TdTomato IX Membrane Palmitoylation TdTomato Connie Cepko 22479 FUmGW Membrane...118737 pBOB-CARMIL2 BH domain-tdTomato Plasma Membrane CARMIL2 BH domain tdTomato John Cooper *Fusions to other...

  16. Hot Plasmids - October 2020

    Type
    Blog Post
    ...you'll find in this post: Tools for labeling excitatory inhibitory synaptic proteins Fluorescent protein...corner New from the viral service Labeling excitatory and inhibitory synaptic proteins with FingRs ...developed AAV vectors to selectively visualize excitatory and inhibitory synapses in the brain.  ...scaffolding proteins PSD95 and gephyrin to label excitatory and inhibitory synapses, respectively. Once expressed... fluorescent proteins targeting inhibitory or excitatory synapses or that are Cre-inducible, researchers... cycle in vivo, particularly if the cells are migratory or in deep tissues. To resolve this issue, Shangqin...

  17. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...support educators interested in integrating MDH-related research into their teaching laboratories. They ...professional development, membership to a local hub of educators teaching MDH CUREs, and opportunities to mentor...

  18. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ... the notation works? The + operator denotes intersection and the – operator denotes set difference. That...inclusive markers of regulatory regions in the genome, including enhancers, promoters, insulators, and more. I...enhancers, lncRNAs, repetitive elements, repressors, insulators, and more. We know that this matter exists, but...criteria: no TTTTT allowed (this is a polymerase terminator), and no off-target effects for the identified...

  19. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...discovered the modular format of some transcriptional activators: that the DNA binding domain (DBD) and transcriptional...of whether it interacts with a transcriptional activator (1a) or a repressor (1b). Scientists took advantage... original Y2H systems utilized the yeast Gal4 activator, bacterial LexA DBD and the lacZ reporter gene...transcription-activating function of a eukaryotic regulatory protein. Science. 1986; 231:699-704. PubMed PMID... and Ptashne, M. A eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor...PubMed PMID: 2547163. 5. Ozawa, T., Kaihara, A., Sato, M., Tachihara, K., Umezawa, Y.l. Split luciferase...

  20. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ... concrete happen. You may have more willing collaborators than you realize. Being at a university provides...meetings and take minutes. Get to know your collaborators from other institutions personally on conference...relationship with the perspective of my potential collaborators in mind. Being at a university allows you to...with people at your organization. You can meet collaborators  for lunch, make a point of talking to them ...as messages and keep you up-to-date on your collaborators’ professional developments without directly ...to remind myself to check in regularly with collaborators for example on a holiday or for professional...

  21. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...has a great team of moderators who facilitated scheduling, filtered out inflammatory questions, and even...and the moderators were on top of any comments that were at all trollish. Kudos to the moderators for keeping...lists of forums on particular topics. Volunteer moderators set the rules for these forums and try to keep...days). Redditors ask serious questions What the moderators did not delete or suppress were the large number...

  22. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...calcium indicator (Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs...performance, and applications of near-infrared calcium indicators in this recent review (Shcherbakova et al., 2021...encoded near-infrared fluorescent calcium ion indicator. Nat Methods 16, 171-174 (2019). Matlashov, M..... A near-infrared genetically encoded calcium indicator for in vivo imaging. Nat Biotechnol 39, 368-377...Near-infrared and far-red genetically encoded indicators of neuronal activity. J Neurosci Methods 362,...

  23. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...crop species including potato, corn, tomato, wheat, mushroom, and rice (Khatodia et al., 2016). Despite...https://doi.org/10.1128/mmbr.67.1.16-37.2003 Khatodia S, Bhatotia K, Passricha N, Khurana SMP, Tuteja N (2016... al., 2013). Nuclease dead Cas9 (dCas9) based activators (such as dCas9-VP64) or repressors (dCas9-KRAB...

  24. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...expression. Fusing dCas9 to a transcriptional activator like VP64 or VPR activates transcription, whereas... is their persistence and inheritance. CRISPR activators and repressors are thought to be reversible once...cases, epigenetic modifiers may work better than activators/repressors - dCas9-p300 increased transcriptional...enables high-throughput screening for functional regulatory elements in the human genome. Nature Biotechnology...engineering CRISPR toolkit for probing in vivo cis-regulatory interactions in the chicken embryo. BioRxiv: ...Additional Resources on the Addgene Blog Cas9 Activators: A Practical Guide CRISPR Between the Genes: ...

  25. Plant Plasmids and Resources

    Type
    Collection
    ...systems such as Arabidopsis, wheat, maize, rice, tomato, and a variety of other species. In addition to... genomes using genome engineering; validated in tomato, tobacco, M. truncatula , wheat, and barley - Daniel...metabolic engineering in plants (including tobacco and potato) - Scott Lenaghan. Joint Modular Cloning (JMC) ...

  26. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    ...have begun to use more precise genetic tools: activators, inhibitors, sensors, recombinases, and more....which was recently published in Frontiers in Neuroanatomy.  Here are the three main takeaways from the...achieved in nontransgenic animals by using cis regulatory elements such as promoters and enhancers, multicistronic...multicistronic vectors, and post-translational regulatory elements. Examples of these approaches are given...specificity of the targeted cell population.  Combinatorial strategies can also be used for retrograde tracing...

  27. Important Considerations When Using AAVs

    Type
    Blog Post
    ...cause the least amount of immune response. Any investigator who is using an in vivo animal model should ...identified with AAV2 (5), AAV6 (6) and AAV5 at excitatory connections (3). These facts beg the question...by the use of AAVs? 1) Combined functional and anatomical mapping of circuits In our lab, we use AAV-DREADD...these complexes are so robustly expressed that anatomical assessments can be easily performed too. While...identified this feature of AAVs as a new tool for neuroanatomical tracing (11). With the ability to produce neuron... Floris G., et al. "A fourth generation of neuroanatomical tracing techniques: exploiting the offspring...

  28. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...Hendel et al. 2015). Low detection rates mean the investigator can be relatively certain that their samples...off-target mutations are essential data points for investigators looking to definitively link genotype and phenotype...viable options for multiplex sequencing in many laboratories. Nevertheless, unbiased detection isn’t as sensitive...genome-wide unbiased methods. However, most investigators use single cell clones for in vitro CRISPR experiments... Hough is a Biochemistry PhD candidate in the laboratory of Prof Steve Jackson at the Gurdon Institute...Resources on the Addgene Blog Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate...

  29. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    ...professional-grade design software, like Adobe Illustrator, and I didn’t want to pay for these programs ... Vectr is like a pared down version of Adobe Illustrator. There’s both a web and a desktop version of ...Inkscape Inkscape is the most similar to Adobe Illustrator out of the three options for drawing your own...20,000 icons is designed by professional medical illustrators with input from life scientists. It’s easy to...medical images organized into four main categories: anatomy and the human body, cellular biology, medical specialities...

  30. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...CsChrimson::tdTomato, an optogenetic tool (CsChrimson) attached to a fluorescent indicator (tdTomato), as the...intermediate (d), and sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe. Image ...dendrites of T5 neurons by placing the calcium indicator GCaMP6f in the effector position, as well as to...
Showing: 91 - 120 of 642 results