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We narrowed to 386 results for: nans

Showing: 91 - 120 of 386 results

  1. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...brain samples from the James Trimmer Lab, using recombinant antibodies with different isotypes. (A) Neocortex... affinity reagents like fluorescently-labeled nanobodies or scFvs. Many, many options for fluorescently.... (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...Beuschel, R. T., Kabat, J., Croteau, J., Davis, J., Hernandez, J. M., & Germain, R. N. (2020). IBEX: A versatile...Immunofluorescence Resources on Addgene.org Find recombinant antibodies available at Addgene Protocols for...

  2. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...protocol is composed of steps that separate out contaminants from an impure AAV preparation. The 15% iodixanol...macromolecules. The 40% and 25% steps are used to remove contaminants with lower densities, including empty capsids.... Image adapted from Zolotukhin, S., et al. "Recombinant adeno-associated virus purification using novel...Left image adapted from Zolotukhin, S., et al. "Recombinant adeno-associated virus purification using novel...by silver stain. Note the increased number of contaminants in each fraction. * AAV capsid proteins VP1,...

  3. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...contrast, fluorescence imaging has emerged as the dominant light microscopy contrast technique in modern ...only excites fluorophores within a few hundred nanometers of the glass/water interface. Super resolution...only excites fluorophores within a few hundred nanometers of the coverslip (4). Essentially, an optical...10 fold increase in resolution, down to 10s of nanometers. Thick dynamic samples Ex: 3D cell cultures, ...KI, Sahl SJ, Hell SW. Lens-based fluorescence nanoscopy. Quarterly reviews of biophysics 48, 178-243 (...

  4. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...fluorescently tagged nanocellulose for nanotoxicological studies. Environmental Science: Nano, 6(5), 1516–1526...isothiocyanate (FITC) can be stably combined with nanoparticles, maintaining intensity at ~85% of its original...specificity to ensure accurate tagging. In Fluorescence Resonance Energy Transfer (FRET) applications, the dyes ...

  5. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...CRISPR/Cas9 could be used to edit several autosomal-dominant mutations that have been connected to the early...Chinese Academy of Sciences found that mice with a dominant mutation in a gene that causes cataracts could... chose to explore its potential in mice with a dominant cataract disorder caused by a single copy of a...applications are also possible through the use of nanoparticles. [6].    References 1. Zhang B. 2021. “CRISPR...

  6. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...and the ITRs are retained with the gene in a recombinant AAV (rAAV) vector (Figure 1). Everything else...outside the ITRs gets left behind.    Figure 1: Recombinant AAV vector with ITRs that flank the gene cassette...plasmid DNA with mutated ITR sequences. Using recombinant-deficient bacterial strains such as NEB Stable...individual reads allows for identification of non-dominant ITR sequences.  Restriction digests with the enzymes...

  7. CRISPR Library Amplification

    Type
    Protocol
    ...lower values than Nanodrop, although most protocols have been designed with Nanodrop as the quantification...lentiviral particles. See below for options if the recombinant band makes up a significant proportion of the...solutions. Quantify the individual Maxipreps by Nanodrop, Picogreen, or Qubit. Each different DNA measurement...burden on the bacteria. Bacteria that contain a recombinant plasmid likely grow faster in the closed system...

  8. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    ...auditing insurance, finance and high-tech industries. He has earned both an MBA in finance and a B.S. in accounting... on average 20 PhD’s a year and we have a very dominant presence in the biotech sector in both patent ...effectiveness of the Plan's risk management, control, and governance processes. Prior to Tufts Health Plan, Carl worked...

  9. Hot Plasmids - May 2022

    Type
    Blog Post
    ... The binding of the GFP nanobody to its target stabilizes the TurboID-nanobody protein and allows TurboID...TurboID fused to a conditionally stabilized GFP-nanobody rather than a specific protein of interest. This...GFP-tagged protein (albeit slightly offset due to the nanobody-GFP interaction). The modularity of the system...

  10. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...others you’ll encounter include monoclonal and recombinant antibodies). By the end, you’ll have a better...polyclonal antibody, or select a monoclonal or recombinant antibody instead. Otherwise, you might experience...of background noise compared to monoclonal or recombinant antibodies. This is because the polyclonal antibody...antibodies Learn about the advantages of using recombinant plasmid-based antibodies Resources on Addgene.org...

  11. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...neural connectivity (Nassi et al. 2015). The recombinant viruses typically used in neuroscience research...transmitted transsynaptically). This is because the recombinant forms of these viruses typically used in research...characteristics of a G-deficient rabies virus recombinant: an in vitro and in vivo study. J Gen Virol. ...Osakada F, Callaway EM. Design and generation of recombinant rabies virus vectors. Nat Protoc. 2013;8:1583...

  12. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ... determine whether two proteins are within 10 nanometers of each other, or measure the tension in the ...    What is this FRET you speak of? Förster Resonance Energy Transfer (FRET) was originally described...has led to the alternative acronym Fluorescence Resonance Energy Transfer. Unlike the typical excitation...Müller, Sara M., et al. "Quantification of Förster resonance energy transfer by monitoring sensitized emission...

  13. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...might not be the right choice. I spoke with our Financial Operations Specialist, Lucy Hammer, about why ...response to this question came from Lucy Hammer, our Financial Operations Specialist. She said, “I’ve found the...came to Addgene with a goal to learn nonprofit finance, and while I've done that, my big takeaway has ...conversation master knows that although her focus is finance, it is just as important to bring love and fun ...

  14. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...general availability of their recombinant antibodies. Since recombinant antibodies are a newer technology...chose monoclonal antibodies (mAbs) and their recombinant forms (rAbs). mAbs and rAbs have higher homogeneity...ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ...

  15. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...biosensor consists of a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically...://doi.org/10.1016/j.cell.2024.01.028. New recombinant antibodies for neurodegeneration research   By...-MAPT [AT8] and Anti-APP [6E10-A5]. Both are recombinant mouse monoclonal antibodies targeting the human...Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant antibody in human postmortem brain samples. Results...

  16. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors [Preprint]. Molecular... K., Parianos, M., Truong, V., Rostonics, E., Hernandez, A., Boye, S. L., Boye, S. E., Levites, Y., … ...Packaging of genomes can limit production of recombinant AAV-2. Gene Therapy, 6(7), Article 7. https:/... Snyder, R. O. (2010). Characterization of a recombinant adeno-associated virus type 2 Reference Standard...

  17. Of Myc and Men

    Type
    Blog Post
    ...Tagging Plasmid-based Recombinant Antibody Resources on Addgene.org  Find recombinant anti-c-Myc [9E10] ..., 1984). The strategy they landed on was to recombinantly append their proteins of interest with unrelated...have moved to producing anti-Myc [9E10] as a recombinant antibody. Researchers first sequenced the variable...

  18. PhD Applications After COVID

    Type
    Blog Post
    ... program is the right fit for you without the financial and logistical stress that accompanies traveling...faculty, students, and each other. Before COVID-19, financial and logistical obstacles could make touring potential...COVID-19? How are students funded? Are there any financial aspects that are confusing? During a traditional...interviews for a variety of reasons (logistical, financial, etc…) now have options to fully engage in the...

  19. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...repairing DNA double strand breaks – (HR). HR is the dominant homology-directed repair (HDR) pathway in mammalian...your cells. Enrich S-phase cells HR operates predominantly in S and G2 phase of the cell cycle, when a ...20): 1-9 (2020). 10.1038/s41589-019-0432-1 Mehdi Banan. Recent advances in CRISPR/Cas9-mediated knock-ins...2014) 10.7554/eLife.04766 Moreno-Mateos, M. A., Fernandez, J. P., Rouet, R., Vejnar, C. E., et al. CRISPR-Cpf1...

  20. Technical Design of a Western Blot

    Type
    Blog Post
    ...-lot. The highest reproducibility comes from recombinant antibodies (rAbs), which are produced from plasmids...plasmid sequencing. While rAbs are monoclonal, recombinant multiclonal antibodies — mixtures of different...non-specific binding; lot-to-lot variability Some recombinant antibodies Not always available; not always... Monoclonal antibodies Increased cost Recombinant antibodies Not always available; cost varies...non-specific binding; lot-to-lot variability Recombinant multiclonal antibodies Not always available...

  21. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...improve cell adhesion on nanofibrous matrices.” Journal of Nanobiotechnology 13.1 (2015): 1–14. PubMed...Cremer, 2003). References 1. Chapman-Smith, A., & Cronan, J. E. “Molecular Biology of Biotin Attachment ... PubMed PMID: 10064313. 2. Chapman-Smith, A., & Cronan, J. E. “The enzymatic biotinylation of proteins...

  22. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...ligand is attached to a protein binding partner or nanobody that targets the native receptor allowing the ....2021.778900 Mattis, J., Tye, K. M., Ferenczi, E. A., Ramakrishnan, C., O’Shea, D. J., Prakash, R., Gunaydin, L... B., Kadmon, J., Raja, C., Chibukhchyan, A., Ramakrishnan, C., Inoue, M., Shane, J. C., McKnight, D. J...science.aaw5202 Mattis, J., Tye, K. M., Ferenczi, E. A., Ramakrishnan, C., O’Shea, D. J., Prakash, R., Gunaydin, L...

  23. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...long-read sequencing techniques, such as Oxford Nanopore sequencing or PacBio SMRT sequencing, (which differ...long-read sequencing (1-25 kb), such as Oxford Nanopore sequencing or PacBio SMRT sequencing. These longer...Levene SD. Analysis of DNA topoisomers, knots, and catenanes by agarose gel electrophoresis. Methods Mol Biol...plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization and stability...

  24. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... is FRET and what's it good for? Fluorescence resonance energy transfer (FRET) is a biophysical interaction... excited, it can transfer energy directly via resonance to the second fluorophore (the acceptor) if the...PMID: 17512008. 4. Fessenden, James D. "Förster resonance energy transfer measurements of ryanodine receptor...Roger Y. Tsien. "Specific covalent labeling of recombinant protein molecules inside live cells." Science281.5374...

  25. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ..., J Biol Chem 2014 Apr 25;289(17):12005-15. Hernandez et al., Autophagy 2013 Nov 1;9(11):1852-61. ...of interest and co-expressing its replacement recombinant protein with a FKBP domain and a mitochondrial... FRB domain (MitoTRAP). Rapamycin binds your recombinant protein (via FKBP) and then sequesters the protein...Rinehart lab reagents for improved expression of recombinant phosphoproteins Protein phosphorylation is one... Under the direction of Sabine Suppmann, the Recombinant Protein Production group at Max-Planck Institute...

  26. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...with a relatively small amount of DNA (in the nanograms to micrograms scale depending on the experiment...more importantly, the DNA needs to be free of contaminants, such as bacterial endotoxins, that could affect... Another is the possibility of carrying over contaminants from the plasmid prep process into the final...final plasmid eluate. Possible contaminants can sneak in from almost any step of the process and include ...

  27. What's New in CRISPR - December 2019

    Type
    Blog Post
    ... blog posts on prime editing, IgnaviCas9, and Nanoblades), but we hope that this blog series will help...ribonucleoprotein delivery by lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells...’s lab developed a lentiviral capsid-based bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins...

  28. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...Bioluminescence Resonance Energy Transfer (BRET). BRET is similar to Förster Resonance Energy Transfer...heterotrimeric G protein dissociation by bioluminescence resonance energy transfer 2, or BRET2. With binding of the...

  29. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...conversion is a unique characteristic of recombinant antibodies. Recombinant antibodies are plasmid-based and ...is routinely used to increase the utility of recombinant antibody tools, the resulting chimera may have...

  30. What the HEK?

    Type
    Blog Post
    ...produce recombinant proteins at high levels. In a case where clinical applications of recombinant proteins...., HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol...
Showing: 91 - 120 of 386 results