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TypeBlog Post...Changing Labor Laws Bring Increased Postdoc Wages Our most popular blog post this month shares incredibly important... Read All of Our Editor's Choice Blog Posts Last month there was plenty of excitement at Addgene. Our... laws and their possible repercussions in Gary’s post. Grad School Advice Part 2: Building Community ... School Advice Part 2: Building Community.” This post leads readers to our latest podcast all about graduate... your PhDs. Of course there were many fantastic posts in October. Click on the links below to catch up...use Reddit to discuss your science and more! All Posts from October 2016: Sequencing Options for CRISPR...for anyone considering a postdoc. In “Changing Labor Laws Bring Increased Postdoc Wages,” Future of Research...
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6 Steps to Submitting a Resume That Gets Seen
TypeBlog Post...to you. Many job postings will result in hundreds of applicants the day they are posted. If your resume... requested in the posting Do this online and/or via email exactly as the job posting instructs even if...most job seekers will and should apply for jobs posted online as one part of their job search. This is...to nail the job yourself. See the links in the post for additioanal advice for the science career search...search. You might also enjoy the other posts in the Addgene Blog Scientist Careers Series. ... -
Summer SciComm: Preprints
TypeBlog Post... and are typically free of charge for authors to post. They can be assessed and commented on by readers...Early-career researchers especially enjoy the ability to post a preprint as evidence of their productivity that...modes of scientific communication. Researchers often post a preprint at the same time as, or before, submitting...present some unpublished results at a conference? Post your working manuscript as a preprint too and you...subscriptions to the specialized journals in your field? Post a preprint before you submit to a journal and share...The most often-cited reasons researchers might not post a preprint are reluctance to share their work too...of preprints. Some worry about faulty work being posted as preprints, assuming that peer review is the ... -
Resume Writing for Non-academic Science Careers
TypeBlog Post...their job postings and salary expectations public, so start by looking at these university postings to give...This post was contributed by Theresa Liao of the University of British Columbia. When transitioning from...than 2 pages long. Write your resume for the job posting Don't think a resume is "one size fits all" like...your resume, and tailor the resume for the job posting. For example, if the job asks for experience in...analysis” (word by word) in your resume. If the job posting says “French-speaking is an asset,” mention “speaks...next step. Start thinking now. Start browsing job postings and look at the jobs you are interested in. Consider...Academic Job Search Handbook & Resumes Read Our Blog Post on Submitting a Resume That Gets Seen Thank You... -
Call for Guest Bloggers
TypeBlog Post...can’t do it all ourselves. Therefore, in this blog post we’re taking a moment to reach out to you, our readers...editors will work with you to help hone your blog post for our audience of biological scientists from a... way to get your name out there! Completed blog posts are also great to include on CVs and Resumes. An...interested in applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR/Cas9 Erik...Professor at Albert Einstein College of Medicine. Post: When is a Monomer not a Monomer? The Top Three ... -
Plasmids 101: Restriction Cloning
TypeBlog Post... GelRed Pre and Post (Post recommended) UV Light 0.1 GelGreen Pre and Post (Post recommended) UV or...and Post Visible Light 100 Methylene Blue Post Visible Light 100 Ethidium Bromide Pre and Post UV ... Stain Pre or Post Run? Visualization Sensitivity (ng DNA) SYBR Safe Pre and Post Blue or UV Light...samples or add the stain as the gel is being made (post or pre run in the table above, respectively). Some... will allow you to read over the insert. See our post on how to verify your plasmid for more details. ...Golden Gate or SLIC Cloning Read Other Plasmids 101 Posts Resources on Addgene.org Find the Perfect Empty... -
5 Reasons to Use Reddit for Science Communication
TypeBlog Post...could see the questions being posted as they were coming in (the AMA was posted at 8:00 am and Joanne didn...Reddit, but it wasn’t until one of our blog writers posted his article (Developing Lab Management Software...pretty strict rules about not being too sales-y in posts which falls in line with our general mission to ...evergreen! With all the great questions that were posted, Joanne’s AMA is now a great resource for any scientists...AMA in the future and link to it in future blog posts on these topics. Others can also continue the conversation... communicating face to face with the audience. I post on LinkedIn where profiles are not at all anonymous...other science career topics. However, many of the posts were about toxic advisors, fruitless job searches... -
Cloning
TypeGuide...information and tips check out our Plasmid 101 blog post on TOPO cloning. Back to Top Gibson Assembly (Isothermal... -
Addgene’s Top 10 Blog Posts from 2014
TypeBlog Post... I’ve compiled a list of the Top 10 Most Viewed Posts on our blog from the past year. A quick glance shows...technique has developed and improved. The CRISPR posts written by our guest bloggers, the experts in the...know what topics you want to learn more about. Our post on “Making Your Own Competent Cells” has been extremely.... If you haven’t yet read the following Top 10 posts of 2014, take some time and see what you’re missing...addgene.org if you are interested. Addgene's Most Popular Posts of 2014 How to Design Your gRNA for CRISPR Genome... -
Academic vs. Industry Postdocs
TypeBlog Post...the list is growing. Some post positions as they come up, whereas others post their positions all at once...Updated Apr 6, 2021. This post was contributed by Laura Desrochers, a postdoctoral scientist at AstraZeneca..., have specific web sites for postdoc listings, so the method of posting varies between companies. Like...all careers related blog posts here Find 9 tips for a successful postdoctoral experience Read Joanne Kamen's...seen listed as a requirement on some industry job postings. Also, if you work in industry for any length ...like to work on later. For instance, AstraZeneca posts most of its positions all at once at the end of ...AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry postdoc in neuroscience at AstraZeneca... -
Transferable Skills Guide: Time Management
TypeBlog Post...out time during my day to write a blog post, edit other blog posts, and contact PIs for an upcoming visit... draft of post Reach out to Beth to see how the interviews are going Read over Leila’s post Read over ...over Susanna’s post Upload Eric’s post and give it a final read Browse list of previous depositors from Boston... have draft blog post ready for publication for tomorrow, I’d better upload that post from Eric before... in need of a few ideas, I hope you’ll find this post useful. After a bit of research and thinking about... need to do by 6 pm. Hopefully you’ll find this post useful for making your days, experiments, and projects... -
How Addgene Creates Content
TypeBlog Post...Addgene blog? Or why we make certain videos? In this post, we invite you behind the scenes in our content ...uncommon to see requests for fact checks on a blog post or Tweet, followed by very detailed answers from...our incredible scientists. Expert reviews on blog posts are done on a case-by-case basis, as many of our...research and previously written Antibody 101 blog posts were used, as well as consults with microscopy and...designer not shown. However, for our blog posts, we usually make figures in BioRender or use appropriately...gaps and add to them as techniques evolve. Blog posts get out of date as techniques and knowledge changes...blog topics or even reach out about writing a guest post (email [email protected].) We are here to support... -
Plasmids 101: Protein Expression
TypeBlog Post... Most proteins are then modified via an array of post-translational modifications including protein folding...synthesis of proteins from mRNA and the addition of post-translational modifications Learn more molecular... plans for downstream applications In this blog post we will summarize some of the more common expression...expression of mammalian proteins that require multiple post-translation modifications for proper protein function...promoters (SV40, CMV, and RSV) for robust expression post-transfection. Mammalian systems can express proteins...produce complex eukaryotic proteins with the correct post-translational modifications. There are two types...bacterial cells are not equipped to add appropriate post-translational modifications. In addition many proteins... -
Twelve Amazing Guest Blog Contributors!
TypeBlog Post...who shared his flow cytometry expertise in three posts. An Introduction to Gating in Flow Cytometry, Designing... who shared her flow cytometry expertise in two posts. Flow Compensation and Flow Cytometry Controls ...critical to the development and writing of two blog posts. Plasmids 101: Modular Cloning and Plasmids 101...More resources on the Addgene blog 10 Great Guest Posts We're Thankful For Lucky Thirteen: Guest Contributors... -
9 tips for a successful postdoctoral experience
TypeBlog Post...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at theJanelia... example). This blog post is about how to get the most out of your postdoctoral training experience. ... successful postdoc fellowship. 1. Reverse engineer success On Day 1 of your postdoctoral experience,...your postdoc At the end of grad school, you searched for and eventually identified a postdoctoral mentor...the Howard Hughes Medical Institute. Today's postdoctoral fellows (PFs) face a number of challenges ranging...academia - the main reason most people enter postdoctoral training. Similarly, there are several factors...factors to consider when selecting a postdoc mentor and lab. These topics have been addressed in numerous... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog Post...better communicated the science in my blog posts. This post highlights several of these free tools which...Researcher Toolbox blog post on social media for scientists Find science career blog posts Learn about other...usually the last thing I thought about when writing posts. Since then I’ve realized my figures are just as...options for drawing your own figure discussed in this post. It’s open source and available for desktop use ... -
What's New in CRISPR - December 2019
TypeBlog Post...What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest bloggers. Check... tools more in-depth (such as these recent blog posts on prime editing, IgnaviCas9, and Nanoblades), but...Check out this blog post for more information. Additional resources on the Addgene blog Download the...the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org... -
Depositor Week 2020 is Happening Now. Join us!
TypeBlog Post...this blog post includes data until November 9, 2020. Between then and the posting of the blog post, we just...: Depositor features (plasmid deposits and blog posts showcasing the work of depositors) Wednesday: Why... -
Plan Your Experiment
TypeGuide...each of these techniques can be found in our blog post CRISPR 101: Validating your Genome Edit . Publications... -
Transferable Skills Guide: Career Planning Resources
TypeBlog Post... facing students today is that many graduate and post-doctoral programs lack any formal or mandatory professional...employer’s career pages to look for current job postings. You may then reference the connection you made...fair when you formally apply for the position. This post on Business Insider nicely summarizes good job fair...monster.com, where you’ll find a variety of listings. Post your CV and make it visible, which will sometimes...well-groomed LinkedIn page. Here you can search for jobs, post your CV, and, most importantly, manage your professional...that you should follow, nicely summarized in this post on Forbes.com, and here on LinkedIn.com. Mentors...in your success. If you are a graduate student or post-doc in a traditional training program, and you hope... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University....University. In this second post in our quick guide to working with Drosophila, you’ll learn how to maniupate expression...your favorite gene (YFG) in flies. Read the first post here. Once you’ve identified some fly stocks and...more details on making a fly line in the next blog post). For example, the hedgehog gene can be overexpressed...sometimes you’ll have to make your own. In my next post, I’ll explain the relatively straightforward process...publication references and web links from my first post and start designing your first fly experiment! ... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post...other posts on scientific communication Find more tips for scientific networking Read this blog post for...This post was contributed by Brittany L. Uhlorn, a PhD Candidate at the University of Arizona. Perhaps...standing at your poster – it’s a different experience to practice while seated or with your poster on your computer... to hold up your poster, and pack a few business cards to tack up next to your poster. Arrive early: You...before your poster session, or it may be held at an unfamiliar venue. Finding your poster board well ahead... your audience. Use your poster as an aid: Have you ever gone up to a poster where the presenter spends...time talking to the poster, as opposed to engaging with you? Be sure to use your poster as a visual aid, ... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog Post...blog post about genome-wide screening using CRISPR Find all of our CRISPR pooled library blog posts Check...This post was contributed by the Open Repository of CRISPR Screens. Imagine you’ve just discovered that...guest bloggers from the BioGRIDS ORCS team. This post is a joint effort by the BioGRID ORCS team. For ...Check out all of our CRISPR blog posts Resources on Addgene.org Read our CRISPR guide Learn more about... -
What's New in CRISPR - Winter 2018
TypeBlog Post...detecting SNPs. Learn more from this Addgene blog post Read the paper in Science Find the Cas14 plasmids...now possible. Learn more from this Addgene blog post Read the paper in Nature Methods Find the anti-CRISPR...be included in the next What’s New in CRISPR blog post, please let us know! Additional resources on the...Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...FOR Boston 2015 Blog Post Read Our Career Blog Posts Read Our Networking Blog Posts ...information about the postdoc work force, problems start with the question of “What is a Post-doc?” The National...biological research enterprise going forward, in this post I’ll point out some of the highlights and takeaways...during the symposium was “How many graduate students/post-docs?” You can find some of the more detailed questions...wanted to touch on in organizer David Riglar’s blog post. Essentially the goal was to determine what data... there is not particularly good data on how many post-docs there are in the U.S., their makeup (in terms...commonly thought of as postdocs. Unfortunately, even when universities have simple postdoc numbers sorted out... -
Grad School Advice Part 2: Building Community
TypeBlog Post...feedback 15:38 - 17:06 : Post Grad Plans 17:07 - 18:29 : How did you hear about post grad opportunities and...them? 18:30 - 19:54 : How to think about picking a post-doc project 19:55 - 22:40 : Improvements that can...Improve the Research Process Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...1722491115. Addgene blog posts related to chromatin This is not the first Addgene blog post about chromatin--...very much 3-dimensional, as you can see by this post’s chromatin illustrations from Leah Bury of Microscopic...following lists of PIs, articles, genes, and blog posts are not comprehensive, so just because you do not...Addgene colleagues and guest bloggers have written posts describing new methods and tools for studying chromatin... have been considering some new techniques, the posts below may steer you in the right direction! CAPTURE-ing...are partially curated from the presentations and posters I saw at the 2018 Keystone Chromosome Architecture... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post...the Addgene blog Check out this blog post on navigating the poster session Find tips to make the most of...your conference attendance Read all of our blog posts about conferences ...crafting your own poster. What sets a scientific poster apart? First, a good poster should catch the ...this type of poster using Powerpoint. “Better Poster.” This new take on scientific poster design was conceived...conference, you need to first make your poster. While a poster generally contains the same sections as... how different posters can be. You have also probably thought about why certain posters resonate with ...tips to help you build a great poster. Tips for designing your poster Check conference guidelines To... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...This post was contributed by Future of Research Executive Director, Gary McDowell. On Dec 1st, the threshold...them overtime accordingly. Read Additional Career Posts on the Addgene Blog How did this change in labor...However when the ruling was issued, a joint blog post by Director of the NIH Francis Collins and Secretary...New Career in Science with Our Career Readiness Posts Learn Good Management for Scientists Resources ...get rid of postdocs; that current postdoctoral appointments may shorten in length; that postdoc salaries...slides here) there are many postdocs paid at much lower salaries). Postdocs (who are not in a primarily...exactly how postdoctoral salaries are changing. Transparent salary information for postdoctoral positions... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog Post...This guest blog post was contributed by Seán Mac Fhearraigh, PhD, of www.phdadvice.com. I sold out, well...pack it in. I had just started month 8 of my 3 year Post-Doctoral contract at the University of Cambridge... other job descriptions for marketing positions posted on recruitment websites, this job specifically ...was a PhD student at University College Dublin & a post-doc a Cambridge University where he studied mechanisms...Macfhearraigh. Read more posts about Scientific Career Development ...Studying for interviews is really important. For my Postdoc position, I tried to read at least 50 papers on... postdoc...