We narrowed to 191 results for: CO

1. Hot Plasmids: Fall 2024

   Type

   Blog Post

   ... fluorescence markers like GFP (510 nm), allowing co-imaging. And because bioluminescent markers do not...

2. Overcoming the AAV Size Limitation for CRISPR Delivery

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   ...packaging capacity but necessitating purification and co-infection of two AAVs. Cas9 orthologs: shorter, ...

3. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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   ... General Meeting (now Microbe) in 2013 in Denver, CO. While I had been to the General Meeting seven other...

4. Tips for CRISPR Gene Editing in Mice

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   ...BbsI site. (c) The pCAG-EGxxFP target plasmid was co-transfected with pX330 plasmids containing sgRNA ...

5. CRISPR Plasmids - Empty gRNA Vectors

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   ...present on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid ...Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI pCRISPR 42875...

6. Quick Guide to Near-Infrared Fluorescent Proteins

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   ...exogenous BV and after normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16...

7. CRISPR 101: Any Base Transversion Editors

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   ... activity. To drive selective A → T editing, they co-expressed Polη, a translesion synthesis polymerase...

8. Antibodies 101: Beyond Surface Labeling

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   ...killer”) cells. Typically, you would label the CD8 co-receptor protein on the surface of the CD8+ T cells...

9. Harnessing Bacterial Toxins for Allelic Exchange

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   ... allow stronger counter-selection than one merely co-opted toward that aim (i.e. sacB). Importantly, to...

10. Five Popular Model Organisms

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    ...Saccharomyces cerevisiae to human: The important gene co‑expression modules." Biomedical Reports 7.2 (2017...

11. Plasmids 101: Biotinylation

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    ...biotinylated (more on this below) it can be incubated or co-expressed with avidin or a structurally similar protein...

12. Hot Biosensors 2022: Year-End Roundup

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    ... (ITAM). By fusing ITAM to an RTK of interest and co-expressing a cytosolic ZtSH2 reporter, they observe...

13. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    ...type’ control can be made one of two ways. First, co-expression of a suppressor tRNA (SupD) instead of...

14. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    ...each due to size limitations associated with AAV; co-infection with both constructs produces a minimum...

15. Colony Formation Titering Assay

    Type

    Protocol

    ...pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...

16. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    ...doi.org/10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2 Uphoff CC, Drexler HG (2011) Detecting Mycoplasma...

17. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

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    ...targeted cytosine methylation in mammalian cells. Co-expression markers EGFP and PuroR enable sorting ...

18. Antibodies 101: Multiplex Immunofluorescence

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    ...interesting protein targets and you want to know if they co-localize at the same place in the cell? Or you know...

19. Technologies Enabled by NanoLuc® Luciferase

    Type

    Blog Post

    ...design a better fluorescent protein that could be co-imaged with GFP using the same wavelength for excitation...

20. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    ...below). In the absence of extra recombinase-specific co-factors (which should be excluded from the system...

21. Virus Protocol - Generating Stable Cell Lines

    Type

    Protocol

    ...pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...

22. Gamma-Retroviral Vector Guide

    Type

    Guide

    ...described above (envelope, packaging, and transfer) are co-transfected into the HEK293T packaging cell line....

23. Viral Vectors 101: AAV Variables That Matter

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    Blog Post

    ...serotypes utilize a number of different receptors, co-receptors, and other mechanisms to gain entry into...

24. Transferable Skills Guide: Teamwork

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    ...joined Addgene’s Green Team, a like-minded group of co-workers who want to make our organization as environmentally...

25. Antibodies 101: Introduction to Antibodies

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    ...production of functional scFv antibody in E. coli by co-expressing the molecule chaperone Skp. Front Cell...

26. Luciferase Plasmid Collection

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    Collection

    ...upstream of Firefly luciferase. Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-Nanoluc-ccdB...

27. Cancer and the Immune System: Deciphering the Relationship

    Type

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    ...their cell-surface proteome to inhibit expression of co-stimulatory molecules needed for T cell effector ...

28. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    ... two equally sized digestion products (that would co-migrate on a gel) instead of two different sized ...

29. Live and Let Dye: Self-Labeling Protein Tags

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    ...dynamic organelle interactions. a) Labeling scheme. b) COS-7 cell ER labeled with Halo-Sec61β with SiR-CA (magenta...

30. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

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    ...good option. Originally developed by Roger Tsien and co-workers (Griffin, Adams, & Tsien, 1998), these dyes...

31. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type

    Protocol

    ...channel pipette 200–1000 µL single channel pipette CO 2 incubator Pipet controller Hazardous waste container...

32. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type

    Protocol

    ...conical tubes NanoDrop spectrophotometer 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm ...

33. CRISPR 101: Cytosine Transversion Editors

    Type

    Blog Post

    ... enzyme HMCES, either as a fusion construct or by co-transfection. HMCES forms a chemical crosslink with...

34. Typing CRISPR Systems

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    ...W., Hatoum-Aslan, A., & Marraffini, L. A. (2015). Co-transcriptional DNA and RNA Cleavage during Type ...

35. Fluorescent Protein Guide: Subcellular Localization

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    ...known subcellular localization patterns. Useful for co-localization studies....

36. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

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    ...repair   ✓   ✓ ~ ~ ~ Transiently co-express mismatch repair inhibitor MLH1dn Promote...

37. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type

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    ...effectively either, but scientists have devised means of co-opting phage genetic systems to facilitate homologous...

38. Trimmer Lab NeuroMab Collection

    Type

    Collection

    ...Stathmin-2 Rat Mouse IgG2a 140066 Anti-Co-Rest/RCOR1 [K72/8R] Co-Rest/RCOR1 Human Mouse IgG2a 140068 Anti-Kv1.6...2b] GluN2C/NR2C Rat Mouse IgG2a 222145 Co-Rest/RCOR1 [K72/2R] Co-Rest/RCOR1 Human Mouse IgG2a 222146 Calbindin...into mammalian tissue culture cells (e.g., HEK293, COS, CHO), from which the R-mAbs are secreted into the...

39. AAV Production in HEK293 Cells

    Type

    Protocol

    ... pipette Hemocytometer or cell counter Ice bucket CO 2 incubator Pipet controller Hazardous waste container...

40. 22 Hot Plasmid Technologies from 2014

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    ...knocking down your endogenous protein of interest and co-expressing its replacement recombinant protein with...

41. Immunology Research Plasmids and Resources

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    ...molecule presenting its cognate antigen along with co-stimulation activates the T cell, whereas T cell ...anergy are induced when the TCR is engaged without co-stimulation or with a low affinity antigen. TGFbeta...anergy are induced when the TCR is engaged without co-stimulation or with a low affinity antigen. Symbol... HRAS1, K-RAS, N-RAS, RASH1 ICOS inducible T-cell co-stimulator AILIM, CD278, MGC39850 IFNG interferon...