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We narrowed to 438 results for: ERG

Showing: 151 - 200 of 438 results

  1. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ... luciferase substrate, the luciferase transfers energy to the FP, resulting in a fluorescent signal. Since...based on the principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla luciferase...

  4. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ... Dept. of Biochemistry and Biophysics and the Lineberger Comprehensive Cancer Center at UNC Chapel Hill...j.molcel.2014.08.002 Ran FA, Hsu PD, Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue ...

  7. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...batch to batch variability. Once an animal has undergone a terminal bleed to acquire all of its serum, ...https://doi.org/10.2144/btn-2018-0065 Fishman JB, Berg EA (2019) Antibody Purification and Storage. Cold...

  9. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...FUNCAT and PLA PLA was originally published by the Söderberg lab in Sweden as a method for the visualization...14166766. PubMed Central PMCID: PMC300121. 3. Söderberg, Ola, et al. "Direct observation of individual...

  10. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...learned along the way. Own your time When I was an undergrad, I was assigned a paper to give as a journal club... of a very confused group of visiting Chinese undergrads. You get nervous and freeze, or forget a point...

  11. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...and lox511 (GtATACAT). These variant lox sites undergo recombination with other sites of the same type...recombination. I. Recombination between loxP sites. Sternberg, N. and Hamilton, D. 1981. PubMed PMID: 6276558...

  13. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...the Auxin Inducible Degradation System (AID) The Dernberg lab in 2015 adapted the AID system discovered ...  Plasmids for cGAL and split cGAL systems The Sternberg lab in 2016 created cGAL, a GAL-4 based bipartite...

  14. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...The stringent stripping solution uses heat and detergent to denature and remove antibodies but is also ...Tris HCl 62.5 mM Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%...

  15. Zebrafish Plasmid Collection

    Type
    Collection
    ...ortholog in zebrafish, this species has more recently emerged as a model system for human disease and translational...

  16. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ... only hypothetical at this point. More time and energy must be devoted to studying Cas3 and its Cascade...S., Lin, C., Owen, A. L. G., Künne, T., . . . Sternberg, S. H. (2019). Harnessing type I CRISPR–Cas systems...

  18. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...tags into the genome of the pathogenic fungus Aspergillus fumigatus, which has been difficult to modify...mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus.” Fungal Genet Biol. 86: 47-57. PMID...

  21. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    .... Find plasmids from this paper at Addgene. Gootenberg, Jonathan S., et al. "Nucleic acid detection ...plasmids from this paper at Addgene. Abudayyeh, Gootenberg, et al. “A cytosine deaminase for programmable...

  22. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...during exocytotic budding, as the viral particle emerges. Retrovirus life cycle Retroviruses go against...this is where viral vectors and natural viruses diverge. For the viral vector, its job as a tool is done...

  23. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...varieties must stay current with literature and emerging technologies to remain competitive and relevant...scientists that aims to help stay current on new and emerging work by collaboratively discussing recent publications...

  24. MXS Chaining

    Type
    Blog Post
    ... Pubmed PMID: 19363495. 4. Josse J, Kaiser AD, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid... PMCID: PMC4409215. 7. Swartz MN, Trautner TA, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid...

  25. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...https://doi.org/10.1126/science.1231143  Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung....2014.02.001  Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...

  26. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...positioned PAM without undesired bases nearby that may undergo unwanted editing. Just like with base editing, ... and antibody-activator fusion proteins. The Synergistic Activation Mediator complex, generally the strongest...

  27. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress." The EMBO journal...PMC4028491. Van Huijstee AN, Mansvelder HD. Glutamatergic synaptic plasticity in the mesocorticolimbic...

  29. Viral Production at Addgene

    Type
    Blog Post
    ...pool. In addition, the final viral preparation undergoes PCR with primers targeting the transfer plasmid...https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination of ...

  30. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...symptoms of the disease. However, there are some emerging treatments that have shown some positive potential...as these new detection techniques and therapies emerge into mainstream use, they will have a much greater...

  31. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ... work she put into this. 5. Reddit forums are evergreen! With all the great questions that were posted...you’ll see below, it pretty nicely displays her energetic, truthful and helpful style: Joanne also provided...

  33. COVID-19 Resources

    Type
    Collection
    ...Schroeder, S., Krüger, N., Herrler, T., Erichsen, S., Schiergens, T. S., Herrler, G., Wu, N. H., Nitsche, A., ...

  35. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...10.1038/s41587-020-0513-4 Kellner MJ, Koob JG, Gootenberg JS, Abudayyeh OO, Zhang F (2019) SHERLOCK: nucleic...2020.04.20.052159 Zhang, F., Abudayyeh, O. O. & Gootenberg, J. S. A protocol for detection of COVID-19 using...

  37. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...sucrose into a toxic metabolite. Cells that have undergone a double crossover will not contain sacB, allowing...are those where the allelic exchange vector has undergone single crossover and integrated into the genome...

  38. Five Popular Model Organisms

    Type
    Blog Post
    ...genetics, and the nervous system. The fly has also emerged as an important human disease model with potential...and read about new systems being developed for emerging model organisms! References Brenner, Sydney. ...

  41. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...messenger cyclic di-AMP in Gram-positive bacteria (Kellenberger et al., 2013). Other variants of Spinach include... 25337688. PubMed Central PMCID: PMC4244833. Kellenberger, Colleen A., et al. "RNA-based fluorescent biosensors...

  42. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...Maesner C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church GM, Wagers AJ. In vivo gene editing...PMC4335351. 9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...

  44. Antibodies 101: Validation

    Type
    Blog Post
    ...Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder...

  45. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...learning. Serve as a daily mentor for a summer undergrad, rotating grad student or high school student.... the time to develop strategies to rest and re-energize yourself, and to address all of the other parts...

  47. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ... to tell if the dependent variables worked synergistically, antagonistically, were additive, etc.). ANCOVA...   The Bonferroni Correction and the Benjamini-Hochberg procedure are designed to reduce false positive...

  48. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...In the 1960s, Har Gobind Khorana, Marshall W. Nirenberg, and Robert W. Holley cracked the genetic code... a renaissance in the field of health sciences, energy, and agriculture, its portrayal in popular fiction...
Showing: 151 - 200 of 438 results