We narrowed to 232 results for: SPL;

1. Hot Plasmids: FRET-Based Biosensors

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   ... microscope using commercially available beam splitters. In addition to imaging neurons, Griesbeck adds...

2. Learning Technical Skills at a Lab Central Workshop

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   ...presentation describing the technology. Then we split into pairs to actually try out the kit itself. This...

3. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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   ...amenable to use with other bEBP/UAS pairs, and displays high dynamic range. However, the promoter in question...

4. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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   ...production of both types of HMO biosensors and displayed her findings at the Spring 2017 ACS meeting. She...

5. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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   ...PMID: 28704652. 6. V. Rodríguez, D. Didiano, C. Desplan, Power tools for gene expression and clonal analysis...

6. The Power Behind NGS Plasmid Validation: seqWell

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   ...because the sample identity information is always displayed at the individual read level, and because custom...

7. Targeted Mutagenesis with EvolvR

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   ...its native endonuclease activity degrades the displaced strand.   Figure 1: EvolvR consists of ...

8. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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   ...David Liu Lab similarly described xCas9, which displayed the ability to target multiple PAM sequences (...

9. Luminescent Imaging with Nano-lanterns

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   ...very robust, with an eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki...

10. Screening for Successful Genome Editing with Digital PCR

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    ...below. Digital PCR Digital PCR (dPCR) involves splitting up a sample into thousands of physically isolated...

11. Hot Plasmids - May 2022

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    ...work, they have found at least two variants that display increased neuronal expression in the brain while...

12. Antibodies 101: ChIP

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    ...choose, you’ll need to reserve a small aliquot, split into two parts to (1) run a DNA gel to confirm fragment...

13. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    ...is also common. Overlap extension PCR is used to splice together two PCR amplicons based on sequence homology...

14. CRISPR Plasmids - Empty gRNA Vectors

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    ...pLSC-5 62889 Mammalian/Lentiviral BsmBI yes, cut (split) S. pyogenes Zhang pICSL01009::AtU6p 46968 Plant...

15. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    ...the Uppalapati and Woolley lab developed a phage display technique using small scaffold proteins to identify...

16. Choosing the B(right)est Fluorescent Protein: Photostability

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    ...top of photobleaching, fluorescent proteins may display reversible intensity changes (Shaner et al, 2008...

17. Transferable Skills Guide: Public Speaking

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    ...the nerve to do what I did. Until that panicked, split-second decision, I had always thought I had an obligation...

18. The time and cost required to make a plasmid

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    ...same time period. For ease of calculations*, let’s split the difference and put scientific inflation at… ...

19. CRISPR 101: Cas9 vs. The Other Cas(s)

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    ...cleavage and 2). cell toxicity. Cas7-11 does not display collateral RNA cleavage and is significantly less...

20. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    ...SpCas9 N692A/M694A/Q695A/H698A). This new variant displays comparable on-target activity and similar or decreased...

21. Overcoming the Challenges of Lentiviral Production

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    ...reduced. Third generation packaging systems further split the viral genome by removing the viral Rev gene ...

22. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating viral genes and transgene...

23. Progress Towards a PAM-Free CRISPR

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    ... reliability. Notably, the SpRY variant didn’t display higher off-target effects than SpCas9, a pleasant...

24. Viral Vectors 101: Virus Safety

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    ...or face shields are required whenever there’s a splash or spray risk in BSL-1. In BSL-2 labs, eye protection...

25. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...chicken beta actin promoter, and rabbit beta-globin splice acceptor, contains sequence that is over 80% GC...

26. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    ...no detectable editing. Additionally, off-target spleen editing was not detected and Nanoblade injection...

27. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    ...direct evolution, capsid shuffling, and peptide displays. These capsids will be selected both for their...

28. Cpf1 Update: Comparison to Cas9 and NgAgo

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    ... cleavage. Now, two new studies show that Cpf1 displays lower off-target editing than Cas9, confirming...

29. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    ...happens often because the HDR inserted sequence splits up the guide sequence.) If neither of the above...

30. Magnetic Control of Proteins: More than a Dream

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    ...) and to see if any other fluorescent proteins display a similar effect (many do). Fusing EGFP to the ...

31. Cloning Mammalian Cells with the Agarose Method

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    ...efficiency is not known, this is easily achieved by splitting transfected cells into 3 plates at different densities...

32. Of Myc and Men

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    ... E (2020) The Myc tag monoclonal antibody 9E10 displays highly variable epitope recognition dependent ...

33. PITChing MMEJ as an Alternative Route for Gene Editing

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    ...clones, they found that 80% and 50% of clones displayed proper insertion at the 5’ and 3’ junctions, respectively...

34. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    ...the number of plasmids matching each filter is displayed in parentheses.  In this example I wanted to find...

35. Summer SciComm Series: Modes of Communication

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    ...  No visuals. Variety of hosting platforms can splinter audience. Primarily digital distribution (though...

36. Lentiviral Vector Uses and Overview

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    ...in two key ways. First, the packaging system is split into two plasmids: one encoding Rev and one encoding...

37. Transferable Skills Guide: External Collaborations

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    ...partnership with SnapGene, for example, allows us to display high-quality maps and annotations without us having...

38. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    ...reprogrammed immediately after collection [2]. PBMCs display unique epigenetic signatures that are closer to...

39. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    ...its translation either by blocking initiation or splicing. (RNAi, in contrast, engages existing gene regulatory...

40. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    ...such as those that affected gene expression, mRNA splicing, or protein coding regions. In total, they tested...

41. Plasmids 101: Plasmid Incompatibility

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    ...plasmids. This strategy has been successful in displacing, or “curing” the virulence plasmids from the ...

42. Plasmids 101: Shuttle Vectors

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    ...was honestly quite boring. My Bio 101 professor displayed a codon table — a chart every molecular biologist...

43. Mesothelioma - Causes, Symptoms, and Treatment

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    ...drugs used to treat mesothelioma – pemetrexed and cisplatin –because it cuts off the blood supply to fast-...

44. 5 Reasons to Use Reddit for Science Communication

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    ...the AMA - as you’ll see below, it pretty nicely displays her energetic, truthful and helpful style: Joanne...

45. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    ...editing, MECP2 reactivation, RNA editing, RNA trans-splicing, and protein replacement to reverse the impacts...

46. Plasmids 101: The Promoter Region – Let's Go!

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    ...chicken beta actin promoter, and rabbit beta-globin splice acceptor. TRE General expression mRNA Tetracycline...

47. Adeno-associated Viruses (AAVs) for Genome Editing

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    ..., the preferred vector, contains a promoterless splice acceptor-IRES-neoR gene, which enhances the efficiency...

48. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    ..., Alexander, et al. "Visualizing the Fate of Transplanted K14-Confetti Corneal Epithelia in a Mouse Model...

49. Plasmids 101: Biotinylation

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    ...bacterial fusion proteins Learn how biotin is used in Split-BioID: An improved method for studying protein-protein...

50. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    ...assay. This reaction also contains SSB, strand displacement DNA polymerase, recombinase, and the ssDNA-FQ...