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Showing: 151 - 200 of 272 results

  1. AAV Molecular Tools

    Type
    Collection
    ...Ready-to-use AAV available from Addgene's viral service encoding tet-off transactivators and tools for... Viral Vector Packaging Service AAV Molecular Tools Viral Vector Packaging Service: Molecular...Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible tools/controls and ... Gradinaru Tools for Affinity Purification These AAV encode tools for affinity purification (which can...Nectow , Eric Schmidt Neurophysiology Tools These AAV encode tools that can be used for neurophysiology...Name Expression System Activity Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven...mapping. 5 Jinhyun Kim Tools for Cell Ablation These AAV encode tools that can be used for cell ablation. ...

  2. AAV Packaged on Request

    Type
    Collection
    ...using AAV Packaged on Request, scientists can start experiments as soon as they receive their AAV prep ...can be ordered in seven serotypes: AAV1 AAV2 AAV5 AAV6 AAV8 AAV11 AAVrg Want more information before choosing...Discover our AAV Packaged on Request service with all-in-one pricing for viral vector preps, which includes...Viral Vector Packaging Service Packaged on Request AAV Packaged on Request Overview Details Ordering Share...Plasmids Additional Resources Adeno-associated virus (AAV) vectors are safe and useful gene delivery tools ...effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both in vitro...choosing a serotype? Learn more about AAV serotypes and tissue tropism on Addgene's blog . All-in-One Pricing...

  5. AAV for Neuronal Tracing

    Type
    Collection
    ... Tracing These AAV encode tools for rabies virus-based monosynaptic tracing. These AAV can be used with...steps for using AAV and modified rabies virus (RABV) for monoclonal neuronal tracing. (1) AAV encoding TVA...deletion-mutant rabies virus AAV1 Ian Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be ...virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...Ready-to-use AAV available from Addgene's viral service encoding tools for monosynpatic retrograde neuronal... Viral Vector Packaging Service AAV Monosynaptic Neuronal Tracing Viral Vector Packaging...to the starter cell in trans , via delivery by an AAV. Since this starter cell now has all the viral genes...

  6. Chemogenetics AAV Preps

    Type
    Collection
    ...Service AAV Chemogenetics Viral Vector Packaging Service: Chemogenetics AAV Use our chemogenetics AAV to chemically... labs. See the Retrograde AAV Inventory Page . Browse all Chemogenetics AAV Don’t See What You’re Looking...Constitutive (non-cre dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor...fewer filtering options. *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from...Ready-to-use AAV available from Addgene's viral service encoding chemogenetics tools. Viral... fusion Flp-dependent 8, rg* Ulrik Gether 119741 AAV SYN flex PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition...IRES EGFP Cre-dependent 5, 9 Scott Sternson 119742 AAV SYN PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition ...

  7. Control AAV Preps

    Type
    Collection
    ...Packaging Service AAV Controls Viral Vector Packaging Service: Control AAV Browse our control AAV that express...options. Browse all Control AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from...provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection, including...Looger 45185 AAV-EF1a-BbTagBY EF1a TagBFP and EYFP Cre dependent 9 Joshua Sanes 45186 AAV-EF1a-BbChT EF1a...These AAV can be used to compare the activity of different serotypes. Serotype AAV1 AAV2 AAV5 AAV6 AAV8...AAV8 AAV9 AAVrh10 AAV11 Retrograde AAV PHP.eB PHP.S PHP.V1 CAP-B10 CAP-B22 MaCPNS1 MaCPNS2 AAV9-X1.1 Promoter...Roth 51506 AAV phSyn1(S)-tdTomato-WPRE hSyn tdTomato Constitutive 5 Hongkui Zeng 58909 pAAV-GFAP104-mCherry...

  8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression, worm...in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain. For plasmids...

  9. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...CMV-driven; CROPseq) Mammalian AAV pRDA_889 (ITRs from AAV2) Mammalian Transposon pRDA_...Mammalian or drosophila) Delivery type (lentivirus, AAV, transposon, Plasmid) Destination vector (dropdown...

  11. Viral Vector Guides and Plasmids

    Type
    Collection
    ...into the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated viral (AAV) vectors are commonly...on Request service for made-to-order AAV vectors from Addgene's AAV plasmid collection. Addgene also has... generally don't integrate into the host genome. AAVs are limited by their small packaging capacity. Gamma-retroviral...suited for specific research goals. For example, AAV vectors are typically preferred for in vivo studies...Stable RNA < 8 kb 80–130 nm Dividing Yes Moderate AAV Transient or Stable* Single-stranded linear DNA ∼...105 nm Dividing/Non-dividing No High *Recombinant AAV has a low frequency of target cell genome integration...

  12. Zhang Lab CRISPR Page

    Type
    Collection
    ...Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery AAV - Cas9 Mouse CRISPR ( C lustered R egularly... lung adenocarcinoma. Delivery of a single AAV vector ( AAV-KPL ) in the lung generated loss-of-function...nature14299. Epub 2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin...#60958) contains pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA...This plasmid is used as a control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR... Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome Editing The Zhang Lab has both single...Cas9 and sgRNA in vivo via adeno-associated virus (AAV). The single vector system uses a smaller Cas9 from...

  13. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...You may also like: Viral Service: All AAV Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida... Viral Vector Packaging Service AAV University of Florida Serotype Testing Panel for ...viral vectors undergo quality control, including AAV titration by ddPCR, in vitro and (when possible) ...transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Mol Ther . 2009 Mar;17(3):463-71...One . Jun 8;10(6). PMID: 26052939 Lu, et al. 2016. AAV-mediated transduction and targeting of retinal bipolar...Rodriguez-Estevez, et al. 2020. Transduction optimization of AAV vectors for human gene therapy of glaucoma and their...

  15. Optogenetics AAV Preps

    Type
    Collection
    ...Browse all Optogenetics AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from...high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV collection, ...Packaging Service AAV Optogenetics Viral Vector Packaging Service: Optogenetics AAV Optogenetic tools ...20071 pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9 Karel Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell...28017 AAV-CAG-hChR2-H134R-tdTomato CAG ChR2/H134R tdTomato Constitutive rg* Karel Svoboda 75470 pAAV-CAG-FLEXFRT-ChR2...Boyden 62726 pAAV-Syn-Chronos-tdTomato Syn Chronos tdTomato Constitutive 8 Edward Boyden 130909 AAV-CAG-FLPX-rc...Deisseroth 22222 AAV-FLEX-Arch-GFP CAG Arch GFP Cre dependent 1, 9 Edward Boyden 28305 pAAV-FLEX-ArchT-tdTomato...

  16. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...viral vector technologies. Penn Vector Core AAV Vectors Many AAV vectors from the Penn Vector Core have been...Viral preparation options include lentivirus and AAV. Viral...ready to ship immediately after MTA approval. Browse AAV Browse Lentivirus Packaged on Request Choose from...reports from the research community demonstrating AAV performance under various experimental conditions...

  17. E11 Bio PRISM Collection

    Type
    Collection
    ...new window) More Resources at Addgene: AAV Packaged on Request AAV Guide Cre-Lox Plasmid Collection Content...How to Use PRISM Barcodes are delivered as pooled AAVs either directly to a region of interest or systemically... such as PHP.eB. The plasmids can be packaged as AAVs and pooled together for co-delivery. Alternatively...at equimolar ratios and packaged together in one AAV preparation. The depositing lab recommends keeping...PRISM workflow. A) Stochastic infection of pooled AAVs delivered in vivo generates unique combinations ...

  18. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...systems have enzymes small enough to fit into a dual AAV system, albeit with lower knockdown efficiencies....HITI and HDR. PASTE is also compatible with both AAV and adenovirus templates. The system, which is larger...

  19. Hot Plasmids - November 2023

    Type
    Blog Post
    ... ideal for size-limited AAV delivery. PE6c and PE6d are optimized for dual-AAV systems. These variants...

  20. Summer Fun at Addgene!

    Type
    Blog Post
    ...Navigating an Expanding Landscape for Engineered Systemic AAVs” with the director of CalTech’s CLOVER lab, Tim ...

  21. Editor's Choice, July 2016

    Type
    Blog Post
    ...Gendered Innovations: Why Does Sex of the Cell Matter? AAV: A Versatile Viral Tool for Gene Expression in Mammals...

  23. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...-9-PV2453 45185-AAV9 AAV-EF1a-BbTagBY Control Joshua Sanes AV-9-PV2454 45186-AAV9 AAV-EF1a-BbChT Control...18917-AAV5 AAV-FLEX-rev-ChR2-tdtomato Scott Sternson AV-5-20071P 20071-AAV5 pACAGW-ChR2-Venus-AAV Karel Svoboda...18916P 18916-AAV9 AAV-FLEX-rev-ChR2(H134R)-mCherry Scott Sternson AV-9-18917P 18917-AAV9 AAV-FLEX-rev-ChR2... AV-5-PV2432 22222-AAV5 AAV-FLEX-Arch-GFP Ed Boyden AV-1-ALL864 51503-AAV1 AAV pCAG-FLEX-tdTomato-WPRE...1-27056 27056-AAV1 pAAV-Ef1a-DIO EYFP Control Karl Deisseroth AV-1-ALL854 51502-AAV1 AAV pCAG-FLEX-EGFP-WPRE...9-27056 27056-AAV9 pAAV-Ef1a-DIO EYFP Control Karl Deisseroth AV-9-ALL854 51502-AAV9 AAV pCAG-FLEX-EGFP-WPRE...-ALL854 51502-AAV2 AAV pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng AV-2-PV0101 105530-AAV2 pAAV.CMV.PI.EGFP.WPRE.bGH...

  24. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Collection Caltech Systemic Capsids AAV Resources AAV Packaged on Request AAV Guide...Institute for Brain Science AAV Enhancer Collection Allen Institute for Brain Science AAV Enhancer Collection ...Find AAV enhancer plasmids for fluorescent labeling and recombination across brain regions and populations...Collection Plasmids Publications Resources Enhancer AAV plasmids are plasmids which use genomic regulatory sequences...across brain regions and populations using systemic AAV delivery. The collection includes the top vectors...window) . Figure 1: Selection, prep, and testing of AAV enhancers in mice. Enhancers were selected from the...accessibility across different cell types and cloned into an AAV backbone. Most enhancers were derived from human,...

  26. An Introduction to Adenovirus

    Type
    Blog Post
    ...immune response to Adenovirus (and/or to its partner, AAV) or 2) a sociological effect of the COVID-19 lockdowns... (tens of kilobases; compared to only 4.7 kb for AAV, 9 kb for retroviruses). The tradeoff is that adenoviral...(AdV-41), or Adenovirus-Associated Virus type 2 (AAV2) – or some unprecedented combination of the three...

  27. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...Views Guides CRISPR Guide Lentiviral Guide AAV Guide Optogenetics Guide γ-Retrovirus Guide ...Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids Blogs Origin of Replication What...

  28. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... the AAV1, AAV5, AAV6, AAV8, AAV11, or AAV Retrograde serotypes and 2 x 10 12 GC/mL for the AAV2 serotype...Packaging Service Packaged on Request Scope of Service AAV Packaged on Request: Scope of Service Addgene's Packaged...and limited use label licenses (LULLs). Titer For AAV preps produced through our Packaged on Request service...cases, we may expect lower titers for a particular AAV prep, mostly likely due to one of the following reasons...

  29. Plan Your Experiment

    Type
    Collection
    ... collection of in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector...delivery. Both lentiviral and AAV vectors are safe to use in the lab, but AAVs exhibits the lowest immunogenicity... transgene expression with AAVs can be long-lived, despite recombinant AAVs not integrating into the host...also frequently use AAVs to develop CRISPR-based therapeutics. One limitation of AAV vectors is that the...blog post on overcoming the AAV size limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged...delivery, lentiviral and adeno-associated virus (AAV) vectors are the most common. Using viral vectors...vector) is smaller than that of lentivirus. Thus, AAVs are generally only compatible with smaller Cas enzymes...

  30. Chemogenetics Plasmids

    Type
    Collection
    ...collection. For example, search for "hM4D mCherry", "KORD AAV", "LMO", "Sternson", or other terms. Looking for ...for viral preps? We also offer ready-to-use AAV preparations of many chemogenetics plasmids. ID Plasmid ...Chemogenetics Guide Viral Vector Packaging Service In-Stock AAV: Chemogenetics Fluorescent Biosensors Fluorescent...

  31. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...about how to minimize leakiness in experiments using AAV vectors. Highlighted Plasmids Browse the tables below...preps feature Cre-dependent tools, or consider our AAV Packaged on Request service for more options. Addgene...Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout...preps feature Flp-dependent tools, or consider our AAV Packaged on Request service for more options. Addgene...CRISPR Knockout Library CRISPR Sidi Chen Use this AAV CRISPR knockout library expressing luciferase and...Return to top Related Addgene Plasmid Collections AAV Enhancer Collection Fluorescent Biosensors Chemogenetics...

  32. Tetracycline Inducible Expression

    Type
    Collection
    ...APEX targeting Alice Ting 85040 pK170.AAV-TRE-Cre-WPRE (Supernova) AAV vector for dox-inducible expression...sgRNA TetR H1-2O2 Nathanael Gray 35625 pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate shRNA...promoter Tet-On 3G rtTA David Vereide 120309 pAAV-FAH-rtTA3G AAV vector to express Tet-On 3G transactivator...promoter TetR Eric Campeau , Paul Kaufman 175274 pAAV-rtTA AAV Tet-On vector with neuron-specific expression...Plasmid #27106 for rtTA. tTA Edward Hsiao 99118 pAAV-CAG-tTA AAV Tet-Off vector, expresses tTA from the CAG... Tet-Off tTA-Advanced Takeshi Imai 104109 pAAV-Syn1-tTA AAV Tet-Off vector, expressed tTA from the hSyn...transactivators and inducible tools or consider our AAV Packaged on Request service available for many transactivator...

  33. Luciferase Plasmid Collection

    Type
    Collection
    ...Schulze-Lefert 83282 pAAV-CAG-RLuc Renilla CAG AAV expression of Renilla luciferase Mark Kay 83280 pscAAV-CAG-RLuc...Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV-CRISPR knockout...William Hahn, David Root 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase...luciferase expression Stephen Tapscott 83281 pAAV-CAG-FLuc Firefly CAG AAV expression of firefly luciferase Mark...Mark Kay 105532 pAAV.CMV.ffLuciferase.SV40 Firefly CMV AAV expression of firefly luciferase James Wilson...expressing luciferase. We also offer ready-to-use AAV preparations of select luciferase expression plasmids...Renilla CAG Vector for packaging self-complementary AAV expressing Renilla luciferase Mark Kay 140328 pLenti-PGK-Venus-Fluc...

  38. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...transgenic DNA exceeding the carrying capacity of AAV vectors." Gene Therapy for Neurological Disorders...Hudry, Eloise, and Luk H. Vandenberghe. "Therapeutic AAV gene transfer to the nervous system: a clinical reality...Maguire, 2016). Recombinant adeno-associated viruses (rAAVs) have become increasingly popular for gene delivery...immune responses (Fenno et al., 2011). However, rAAVs lack the ability to specifically infect defined ... promoters, on the other side, are too long for rAAVs, which can only package sequences shorter than 5...vectors in combination with transgenic animals or rAAVs expressing Cre under a specific cell-type promoter... 2019). For example, Taylor and colleagues used rAAV FLEx-based vectors to study the neural circuitry...

  39. Optogenetics Plasmid Collection

    Type
    Collection
    ...Looking for viral preps? We also offer ready-to-use AAV preparations of many optogenetics plasmids. ID Plasmid...Optogenetics Guide Viral Vector Packaging Service In-Stock AAV: Optogenetics Fluorescent Biosensors Fluorescent ...

  40. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use AAV9 and AAVrg vectors. ...available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON vector 137128-AAV8: Ef1a-Coff...

  42. What the HEK?

    Type
    Blog Post
    ...Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells  ...

  47. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...and to compare different Cas enzymes in all-in-one AAV delivery. While Fragmid developers were specifically...Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo...

  50. Neurodegeneration Research Collection

    Type
    Collection
    ... . (Tian et al. Neuron. 2019) See More AAV Viral Preps Find AAV viral preps for systemic delivery of viral...research. Disease Info Plasmid Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies...
Showing: 151 - 200 of 272 results