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We narrowed to 208 results for: pme i

Showing: 151 - 200 of 208 results

  1. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ..., Isern S, Michael SF, Lorenzana I, Yozwiak NL, MacInnis BL, Bosch I, Gehrke L, Zhang F, Sabeti PC (2018...-2 RNA, but inadequate access to reagents and equipment has become a bottleneck. In the last few years...

  2. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...factors contribute to the popularity of GFP including (i) fast and complete maturation to functional, fluorescent...discussed below. There are numerous GFP variants. Here I use the name ‘GFP’, which refers to mEGFP. This variant...entirely genetically encoded do not exist, but the development of non-natural fluorescent amino acids and their...

  4. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...doi.org/10.1038/s41467-019-13805-y Doench JG (2017) Am I ready for CRISPR? A user’s guide to genetic screens...s41587-020-0490-7 Radzisheuskaya A, Shlyueva D, Müller I, Helin K (2016) Optimizing sgRNA position markedly...is still the most widely-used Cas enzyme, the development of SaCas9, NmeCas9, Cas12a enzymes, and engineered...

  5. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...quickly identify possible gene function. In this post, I will describe a useful and cost-effective method that...Thermo works well) with some added lysozyme, DNAse I, and protease inhibitor cocktail. Line the bottom ...Jessica C. Sacher, and Christine M. Szymanski. "Development of an Assay for the Identification of Receptor...

  6. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...Interest Waiver (NIW) allows for self-sponsorship of the I-140 application meaning that the category does not...and should help facilitate the interview process. I often suggest to international candidates that they...writing, clinical, regulatory, product development, business development, consulting, policy, big data and ...presented may not reflect the most current legal developments.  Further, this post may contain technical inaccuracies...necessary and often overlooked part of career development for foreign national researchers and entrepreneurs... Careers is a life science search and career development firm focused on connecting innovative, entrepreneurial...project management, alliance management, business development, finance, and operations. To learn more, visit...

  7. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...pnas.1915974116 Guerin K, Rego M, Bourges D, Ersing I, Haery L, Harten DeMaio K, Sanders E, Tasissa M, Kostman... Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020) A Novel Next-Generation...classes of neurons transiently express TH during development or have low levels of TH mRNA without expressing...

  8. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ... Career Coaching for Scientists: Why and Where Do I Find One? If you need help making a transition into...the coming years as we stay on top of the many developments in science and the plasmids, technologies, and...

  9. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ... to major histocompatibility complexes (MHC class I and II).  There are multiple algorithms, both open-access....), The Nonhuman Primate in Nonclinical Drug Development and Safety Assessment (pp. 519–542). Academic....2018.06.008Lu, RM., Hwang, YC., Liu, IJ. et al. Development of therapeutic antibodies for the treatment of...

  10. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...researchers to use this tool in their own work. She says, “I spend a lot of time packing, shipping, and sharing...at a variety of questions surrounding cancer development, metastasis, and drug resistance, Bhang says ...appropriate, non-overlapping targets for drug development - such that initial resistant populations can...tumor heterogeneity to drug discovery and the development of combination therapies.         References ...

  11. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...encoded magnesium sensor, the first of its kind. “I hope by depositing at Addgene, people will start using.... Merkx recently applied this “trick” to the development of additional color variants. This resulted in...

  12. Clear and Accessible Writing

    Type
    Blog Post
    ...What is the goal, who is my audience, and what do I want to be different for them when I’m done? What ...online resources. On the backend, our product and development teams have worked hard in increasing and improving... 2023, we hosted our very first professional development workshop, “Make a Greater Impact Using Clear ...

  13. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...just six weeks later. The Zika publication timeline I think we can speak for all the authors on the paper...and rare, but severe, symptoms, including the development of fetal microcephaly and Guillain-Barré syndrome...health emergency and called for the fast-tracked development of diagnostics. Mostly transmitted by Aedes mosquitoes...timescale. Our article ultimately reported the development of a rapid design and prototyping pipeline that...necessary to advance the technology through product development, the regulatory process, and manufacturing so...

  15. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...can later be verified by Sanger sequencing. How can I tell if my enzyme will cut? Whether performing a digest...Blocked by overlapping methylation Not affected PmeI Not affected Not affected Blocked by overlapping...

  16. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... follows are just a few suggestions based on what I have found to be especially beneficial during my own...scientists. Continuing professional development Professional development should be life long, and doesn’t...landing that next coveted job! Professional development (or lack thereof) One of the biggest challenges...programs lack any formal or mandatory professional development training. Further, even for those students and...take responsibility for your own professional development, not count on your institution, program, or adviser... a job fair, and utilizing your local career development center, if one exists. Get online! Review company... career development...

  17. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ... Shah et al., “Do you know the sex of your cell?” I can’t tell you how many researchers I’ve talked to...harm humans or contribute to failures in drug development – costs far greater than those of doing the research...

  18. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...of chemically converting adenosine (A) to inosine (I, treated as G by the cell). Surprisingly, the team..., 843–846. https://doi.org/10.1038/nbt.4172 Kurt, I. C., Zhou, R., Iyer, S., Garcia, S. P., Miller, B....pathways that were considered problematic in the development of CBEs and ABEs. This post includes a lot of...

  21. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...Addgene Viral Plasmids Which Type of Virus Should I Use? Resources on the Addgene blog Viral Vectors...scalpels (sharps). Cuts and scrapes from sharp equipment are one of the most common forms of lab accidents...

  22. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...expression mRNA Yeast promoter for alcohol dehydrogenase I Repressed by ethanol Full length version is strong...only in certain tissues or at certain times in development or there may be ways to turn them on or off at...tables above do not delve into the tissue or development-specific promoters available to scientists. Plasmids...

  24. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ..., Ishibashi, H., Yuasa, S., Takeda, S., Hasegawa, I., and Nakahara, K. (2011). Local and retrograde gene... J. M., Vandenberghe, L. H., Haute, C. V., Thiry, I., Deroose, C. M., Mortelmans, L., Wilson, J.M., Debyser...like these may be a starting point for future developments in the field of AAV vectors. Conclusion An ideal...intracellular transport of AAV could facilitate the development of more powerful AAV serotypes, and perhaps lead...

  25. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ... These Acr proteins were originally found in type I CRISPR-Cas systems.   Four years later, the Sontheimer...Cells. Molecular Therapy - Methods & Clinical Development 9:390–401 . https://doi.org/10.1016/j.omtm.2018.04.008...

  26. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...lifetime of 3.9 ns, quantum yield of 0.70), mScarlet-I with T74I (accelerated maturation in cells compared... such inhibitors have been characterized for type I CRISPR systems, but only recently have inhibitors ...is a red-shifted variant which, with further development, could be better suited for deep-tissue in vivo...

  28. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...doi.org/10.1016/j.ymthe.2022.09.015 Ramani, B., Rose, I. V., Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal... more comprehensive thanks to the consistent development and improvement of CRISPR-based technologies ...

  29. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...Evans, E. W., Déjean, V., Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore, P. J. (...-progress still in need of further study and development like MagLOV. The results with MagLOV raise broader...

  30. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...tools used in research, and data repositories. Below I describe how each of these kinds of connectivity facilitates... modern and fully supported API that enables development of custom integrations with other tools and resources...

  31. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...The first time I heard about FRET during a journal club, my guitarist brain automatically thought about...can imagine not old-fashioned at all. With the development of new fluorescent pairs and the emergence of...

  32. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...deletions, insertions, and even precise point mutations. I think it will always have a place in every bacterial...experiment? Find the pTOX plasmids here! Continued development of new allelic exchange vectors Allelic exchange...clear advantages, and are hopeful that continued development of allelic exchange vectors will produce an array...

  33. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...disulfide bonds, made by molecular evolution 1 1Edited by I. A. Wilson. Journal of Molecular Biology 275:245–253...Principles and Clinical Application. Clinical and Developmental Immunology 2012:1–15 . https://doi.org/10.1155...reagent sharing platform for antibodies, the Developmental Studies Hybridoma Bank Learn more about the ...

  34. An Introduction to Adenovirus

    Type
    Blog Post
    ... Krahl, T., Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune response to adenoviral vector...existing vectors, as well as research into the development of new adenoviral vectors. This post was written...

  35. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...cells. The REPAIR (RNA Editing for Programmable A to I Replacement) system fuses Cas13b to RNA deaminase ....  In 2020, the Kleinstiver lab reported the development of near-PAMless Cas9 variants. These variants...

  36. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...and resources References Doran, K. S., Konieczny, I., & Helinski, D. R. (1998). Replication Origin of ...Tor, X. Y., Loh, P. C., & Wong, H. L. (2023). Development of constitutive and IPTG-inducible integron promoter-based...

  38. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...https://doi.org/10.1038/s41477-020-0704-5 Santana I, Wu H, Hu P, Giraldo JP (2020) Targeted delivery of...plants, including the development of crops with longer shelf life and the development of disease-resistant...

  39. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...shape and the absence of a rigid cell wall. Here, I will cover some of the best ways to tackle mycoplasma...cross-contamination. Make sure you keep all the required equipment and reagents inside the laminar airflow before...cryopreserved cells decreases with every subculture. Equipment Fumigate the laminar airflow and the lab facility...

  40. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...CMV-LUC2CP/ARE and Luc-I (intron-containing; CMV-LUC2CP/intron/ARE). The Luc-I intron is a chimeric β-...splicing. If intron splicing does not occur in Luc-I, the produced luciferase is truncated and lacks enzymatic...pairs in series with unique spacer sequences (pMemoryArray) gives a DNA sequence capable of recording 11...reporter constructs or as the fully assembled pMemoryArray.   Yang et al., Nat Methods 2014 Dec;11(12...transactivators available through Addgene. The development of dCas9 and dCas9-fusion proteins has dramatically...

  41. Optogenetics Guide

    Type
    Guide
    ...Ramakrishnan C, Mattis J, Prakash R, Diester I, Goshen I, Thompson KR, Deisseroth K. 2010 Molecular and...Schneider F, Davidson TJ, O'Shea DJ, Sohal VS, Goshen I, Finkelstein J, Paz JT, et al. 2011. Neocortical excitation... novel ChRs from other algal species and the development of synthetic variants to enhance the functionality...Channelrhodopsin Variants: Features, Limitations and Future Developments. Exp Physiol. 96(1): 19–25. PMID 20621963 Mattis...

  42. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...deaminase catalyzes the conversion of a target ‘A’ to ‘I’ (inosine), which is treated as ‘G’ by cellular polymerases...of donor DNA to facilitate repair. Since the development of CRISPR-mediated base editing (BE), these types...

  43. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... the major histocompatibility complex (MHC) class I proteins on their surfaces to avoid recognition by...tumors and cancer. The immune response to the development of tumors is a complicated and dynamic phenomenon... Equilibrium - In a process analogous to the development of drug resistance in bacteria, the tumor cells... onset of cancer. Similar patterns of cancer development are observed in autoimmune disorders. Chronic...autoimmune disorders is also known to precede cancer development, for example, inflammatory bowel disease is associated...in the various types of cancer will fuel the development of immunotherapeutics. There are still many important...

  44. Lentiviral Vector Guide

    Type
    Guide
    ...Gallay, P., Ory, D., Mulligan, R., Gage, F. H., Verma, I. M., & Trono, D. (1996). In vivo gene delivery and... H., Yu, E. Y., An, D. S., Sabatini, D. M., Chen, I. S., Hahn, W. C., Sharp, P. A., Weinberg, R. A., &....2192803 PMID: 12649500 Timms, R. T., Tchasovnikarova, I. A., & Lehner, P. J. (2018). Differential viral accessibility...understand integration sites have led to the development of multiple genome-wide analysis pipelines to...barcoding libraries . Mouse Modeling Since the development of safer lentiviral vectors, they have been seen...differences. Molecular Therapy — Methods & Clinical Development , 21 , 451–465. https://doi.org/10.1016/j.omtm...sites. Molecular Therapy — Methods & Clinical Development , 20 , 792–800. https://doi.org/10.1016/j.omtm...

  45. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...effective for T cells transduction for instance. I hope you will find this information useful for your...sensors. Retroviral vector background Until the development of viral vectors, gene transfer into mammalian...was a disadvantage particularly for clinical development. In addition, MoMLV is inefficient at infecting...

  46. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...direction and scope of the subsequent two. Consequently, I will focus on different approaches and considerations...Although all these steps are areas of active development, the first step necessarily dictates the direction...: a mechanism underlying genetic robustness. Development 147:dev186452 . https://doi.org/10.1242/dev.186452...

  47. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...PMC516055. 23. Pascal, John M., et al. "Human DNA ligase I completely encircles and partially unwinds nicked ...conditions, and formulations and has led to the development of the new HiFi Taq DNA Ligase. Using this method... PMID: 23142428. 17. Hamada, Mariko, et al. "Development of a ligase detection reaction/CGE method using...PMID: 23463388. 18. Hommatsu, Manami, et al. "Development of a PCR/LDR/flow-through hybridization assay...

  48. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...ever since there were plasmids to share. Back when I was in the lab, if you read a paper and saw an interesting...Hooper, PhD, has also worked with R&D for product development and now specializes in supporting Promega cellular...

  49. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...T. Dargitz, R. Wright, A. Khanna, F. H. Gage, and I. M. Verma, “Functional Gene Correction for Cystic ...efficient manner than ever before, thanks to the development of site-specific nucleases (CRISPR/Cas9, TALENs...

  50. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...gang, Mesik, L., Liang, F., Tao, H. W., & Zhang, L. I. (2017). AAV-Mediated Anterograde Transsynaptic Tagging...vivo. Molecular Therapy - Methods & Clinical Development, 3, 16062. https://doi.org/10.1038/mtm.2016.62...
Showing: 151 - 200 of 208 results