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We narrowed to 949 results for: APP

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Showing: 61 - 80 of 949 results

  1. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...explore alternate approaches. Being a “lazy” geneticist, I developed a co-selection approach relying on repair...with unprecedented ease. These new C. elegans approaches rapidly enrich for editing events without the...phenotypes (unc-119) (Dickinson et al., 2013). These approaches allowed effective recovery of knock-ins, but ...Including Cre-mediated marker excision, it takes approximately 3-4 weeks to obtain a homozygous, outcrossed...several manuscripts published in Genetics detailed approaches in which selection for an editing event that ...and knock-ins at other genomic loci. The first approach – co-CRISPR from Craig Mello’s lab – used inactivation...animals with particular visible phenotypes. These approaches may become even more powerful following a report...

  2. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...protein for your application. Selecting the best fluorescent protein for a specific application can seem like...under conditions that best mimic the intended application. The three key properties for live cell imaging...theoretical brightness. The theoretical brightness often appears in tables with fluorescent protein properties and...experimental set up. The practical brightness takes all application specific parameters into account, including the... picture of what can be expected in a 'real' application than the theoretical brightness. Consequently...brightness in a system closely mimicking the future application. In figure 2 we provide an example of the comparison...

  3. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...cas9 gene and single guide RNAs (sgRNAs) with appropriate flanking homology into a germline cell can create...RNA-guided gene drive. In principle, this event could happen even if there isn't any homology available. Consequently...local biosafety officer should be familiar with appropriate measures. Barriers should be a component of all...eventual release should consider building an appropriate immunizing reversal drive at the same time to...species in question), why not use them whenever applicable?Against this, consider the cost of an accidental...An accidental release could delay real-world applications against scourges such as malaria and dengue .... Disclaimer: Recommendations concerning the appropriate degree of confinement are those of the author...

  4. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ... the app. Technical difficulties? Attending a conference virtually means getting to know the app or virtual...conferences with multiple sessions happening at once, virtual conferences may have apps or interactive calendars...was able to get in touch with the author. “In the app, you can write a message to the author of the poster...there may be a “place” for networking within the app. For example, the NeuroSymposium had virtual tables... puts butterflies in your stomach, the virtual approach might be less stressful. For both conferences,...Having an on-site conference canceled may be disappointing, but there are many creative ways to adapt and...

  6. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...

  9. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...There are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells...based affinity purification and mass spectrometry approaches. Recently however, scientists have developed ...miniturbo Split-BioID: A conditional proteomics approach to monitor protein-protein interactions Split-... PMC3308701. Rhee, Hyun-Woo, et al. "Proteomic mapping of mitochondria in living cells via spatially restricted..., et al. "Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally...proximity-based labeling methods for interactome mapping. F1000Research, 8.  ...

  10. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...Cloning grade DNA is designed for use in cloning applications. Direct transfection into cells is not recommended...

  12. Plasmid Collections

    Type
    Collection
    ...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...

  13. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ..., some CRISPR-Cas3 plasmids take an all-in-one approach and include Cas3 and Cascade on one single plasmid...

  14. CRISPR Plasmids and Resources

    Type
    Collection
    ...fused to deaminases can edit bases in RNA. Other Applications Screen CRISPR libraries are a powerful tool ...

  15. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...and are thus well suited to directed evolution applications. Examples of these base editing systems include...

  17. Viral Vector Guides and Plasmids

    Type
    Collection
    ...strong immunogenic response, useful for clinical applications as vaccines. Selecting a Viral Vector Type Each...

  18. mTOR Pathway

    Type
    Collection
    ...pro-proliferative and anti-apoptotic signals. mTORC2, which appears to signal upstream of Akt, also promotes cell survival...

  19. Chemogenetics AAV Preps

    Type
    Collection
    ...Bryan Roth 65417 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor (KOR) - Inhibition IRES mCitrine...
Showing: 61 - 80 of 949 results