Skip to main content
Addgene
Showing: 61 - 80 of 85 results

  2. March for Science

    Type
    Blog Post
    ...general (1). Science and politics? Science is an apolitical process for seeking knowledge. The process begins...

  5. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...permeabilization with detergents such as triton-x, saponin, or tween. Take care when using strong detergents...

  7. Water Bath Protocol

    Type
    Protocol
    ...on the surfaces of the water bath as the water evaporates from the tub. Disinfectant may be added to prevent...cover on the top of the water bath to prevent evaporation and maintain the desired temperature. This also...

  8. Deep Dive: qPCR

    Type
    Blog Post
    ...configurations can help you identify pipetting or evaporation errors. And if time allows, you can fit in a ...

  9. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...well ventilated area and avoid breathing in the vapors. Workflow Timeline Day 1: Antigen Coating Day 2...Desmin (blue) or a negative control protein, Apolipoprotein L3 (red) overnight, blocked, and incubated ...

  11. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...dead” Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors are targeted to a specific locus ...

  12. mTOR Pathway

    Type
    Collection
    ...the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears to signal upstream...

  13. Qi Lab CRISPR Page

    Type
    Collection
    ...protospacer adjacent motif or PAM, sequence: NGG) juxtaposed to the DNA complementary region. The uniqueness...

  15. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...tuberculosis M. smegmatis Green monkey ( C. sabaeus ) Kaposi's Sarcoma-associated Herpes Virus (KSHV) Cow ( B...

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...processes such as differentiation, proliferation, and apoptosis. In humans, mutations that permanently activate...

  17. Validated gRNA Sequences

    Type
    Collection
    ...GAAGCGGGCAAAGGGGCGAC 58780 cut/nick S. pyogenes 24954249 Yamamoto apoea D. rerio GGATGAGCCAAGAAGCCGCT 42241 cut S. pyogenes...

  20. Western Blot

    Type
    Protocol
    ...BSA standard concentration versus absorbance. Extrapolate the total protein concentration of the sample...
Showing: 61 - 80 of 85 results