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Addgene

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We narrowed to 568 results for: CIT

Showing: 61 - 80 of 568 results

  3. Bacterial Expression Systems

    Type
    Collection
    ...which may aid disulfide bond formation or prevent toxicity. Compatible with Gateway cloning. pGTvL1-SGC 39188...

  4. Brain Initiative Collection

    Type
    Collection
    ...Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven KOR DREADD...

  5. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...speak of excitation and emission spectra. Both spectra possess clear maxima where excitation and emission...laser excites at a wavelength of 488 nm (Table 1), which is near a local maximum of PE's excitation spectrum...laser, however, excites at 561 nm, which is close to the global maximum of PE's excitation spectrum where...signal.   Figure 2: Excitation and emission spectra for PE. The excitation spectrum shows two maxima...the red laser excites at 640 nm (Table 1), while comparing the two fluorophore's excitation spectra around...that APC-Cy7 is excited at a lower efficiency than APC. While APC's global excitation maximum lies around...-Cy7's global excitation maximum is about 750 nm. If we were to use a laser that excites at 750 nm, APC-Cy7...

  6. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...fluorophore primarily depends on the excitation power and the excitation wavelength. A fluorophore is less...bleach when it is excited off the peak wavelength, since it will absorb the excitation light less efficiently...decreasing the power of the excitation light reduces the number of excitation/emission cycles per unit of...acquired at low excitation powers. Note, however, that, at a 14-fold higher excitation power, the photostability...lightbulb or exchanging excitation filters). This avoids substantial changes in excitation power and will help...fluorescent proteins for live cell imaging can be excited many times, thereby producing a large number of...story. The photobleaching rate does not depend on excitation power in a linear fashion. This implies that ...

  7. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ... and thereby provide molecular or structural specificity to the viewer. Finally, modern microscope designs...detector or restricting fluorescence to specific excitation volumes to enhance spatial contrast (i.e. optical...microscopy. Collimated (non-converging or diverging) excitation light exits the microscope objective evenly illuminating... CT scanner. This is accomplished by focusing excitation light to a point in the sample and raster scanning...containing a number of holes rotates through the excitation light path. Each hole corresponds to a different...First, widefield and confocal microscopy project excitation light through the entire axial volume of a sample...signal intensity. 2-Photon microscopes restrict excitation (and bleaching) to a single focal point. This...

  8. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... to an excited state through the absorption of photons. After a brief elevation in this excited state,...objectives. Most systems can only excite and read a set number of excitation and emission wavelengths, as ... or the difference in wavelength between peak excitation spectrum and emission spectrum. Fluorescent dyes...traditional fluorescent protein tags, such as higher specificity, increased versatility in probe attachment, and...chemical structure, leading to differences in their excitation and emission wavelengths, which are pivotal in...dye is fluorescein isothiocyanate (FITC), with excitation/emission peak at 495/517 nm. FITC binds to an...this include coupling to an antibody, whereby excitation of blue light will indicate where FITC is bound...

  9. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ... they elicit an immune response from the body. Several factors contribute to the immunogenicity of a drug...relative immunogenicity score (Mattei et.al., 2022).    Figure 1: Generation of immunogenicity scores...prediction of drug immunogenicity  The goal of in vitro prediction of immunogenicity is to validate the...dose (Lu et.al., 2020).  Evaluating a drug’s immunogenicity in-vitro helps researchers understand a drug...However, in silico and in vitro assessment of immunogenicity can help evaluate risk of an antibody.  In...drug epitopes likely to be presented by T cells to elicit an immune response. T cell epitopes are found in...commercially available algorithm to evaluate the immunogenicity of therapeutics in silico. These algorithms...

  10. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...autofluorescence, and, in the case of exciting cyan-excitable donors, phototoxicity. Another challenge to using ...using the same wavelength for excitation. The result was the cyan-excitable orange fluorescent protein, ...FPs) can be problematic because excitation light can cause phototoxicity, autofluorescence, and perturb...FRET donor excitation wavelengths overlap with optogenetic initiation wavelengths, excitation of the optogenetic...the following characteristics: No photoexcitation High Ion specificity High sensitivity Easy expression...their use is limited in vivo because they must be excited by external light sources. These external sources...with a red-shifted fluor. Here, NLuc internally excites the fluor through a process known as bioluminescent...

  11. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...average number of citations per year and the number of other manuscripts that heavily cite the paper are ...number of citations highly influenced by this paper. Influence is measured by the number of citations and the... the context of these citations. The number of manuscripts that cite the background, methods, or results...author, total number of citations, and the number of publications that heavily cite, or are highly influenced...other authors who they cite the most), and who the author influences (who cites the author the most). ...is essentially a virtual bookshelf. Pro tip The “cited by” and references sections of a PubMed entry are...studying and gives you a sense of what people are excited about. Following scientists in fields outside your...

  12. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...the toxicity of guides that have 1-bp mismatch off-target sites, short guides had reduced toxicity compared...affected by low on-target efficacy, non-specific toxicity, and off-target effects. The flaws of RNAi screens... was validated via a growth screen and a ricin toxicity screen since essential and non-essential genes...identified  60% of the essential genes. The ricin toxicity screen had a 10% false discovery rate and identified...identified 67% of previously identified ricin toxicity genes. The screen also discovered several genes previously...toxic if they were depleted from the screen. This toxicity was used a proxy for detecting Cas9 cutting. Safe...targeting guides better control for non-specific toxicity than non-targeting guides First, Morgens et al...

  13. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    ...Addgene. How to cite options using the different PID formats The instructions for how to cite any Addgene ...have used the same plasmid through the “Articles Citing this Plasmid” section. Having IDs linked to these...reproducibility of experiments. Additionally, proper citation of these Addgene IDs in published work allows ...section have been updated to include the option of citing with resolvable compact identifier and RRID formats...this plasmid’s page you will find that the “How to cite this plasmid” section now lists the multiple PIDs...n2t.net/addgene:87155 ; RRID:Addgene_87155).” So when citing, you can copy and paste the text attributing the... Or if in doubt, copy and paste the entire line. Cite plasmid using a resolvable compact identifier and...

  14. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...your particular field of research.   Citations tools DOI Citation Formatter Just enter the DOI of a paper...generating citations for a few papers at a time. Mendeley Mendeley works much like other popular citation managers...academic databases.  Downloading Mendeley Cite lets you cite papers from your Mendeley library in Microsoft...Zotero is also another free citation manager that lets you organize and cite papers. With the Zotero Connector...Chrome browsers with just one click. Papers can be cited in Word, LibreOffice, or Google Docs. Files can ...chart highlights the differences between these two citation managers. If you want more tips on finding and...podcast episode 2 "Essential Grad School Resources: Citation Managers, Programming, & Productivity": This podcast...

  15. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...wavelength than the exciting wavelength. Thus, FPs are proteins with this unique capacity. Many of these FPs...switch their color when activated by a specific excitation wavelength. This means that the emission wavelength...George G. Stokes) is the shift in wavelength from excitation to emission. For most FPs, Stokes shift is less...shift is ≥ 100nm. Specifically, these proteins are excited by UV light or blue light and their emission is.... Fluorescent Sensors: These FPs change their excitation/emission behavior upon environmental changes ... in mind when choosing a fluorescent protein Excitation & Emission (ex/em): Each FP has its unique ...peak. Therefore, choose FPs that your system can excite, and detect the emission. For example, if your ...

  16. How to Write a Scientific Review Article

    Type
    Blog Post
    ...lot of references. I cite as I write, meaning I use the software to add the citations in real time as I ...and experiments. A comment about citing: I wasn’t sure if I should cite reviews or the primary literature... little crazy (meaning you are probably going to cite hundreds of references) and it’s better to keep ...from the beginning. I also recommend using the citation style of (Last name, Year) in the document while...experiments. Later, you can easily convert the citation style to whatever the journal requires. Using ...studies. I also did not limit myself to the well-cited or popular papers, but looked for papers from a ...anything else. Like I mentioned, I also added citations in real time as I wrote, so each statement was...

  17. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...filter set but the excitation filter excites more of the peak of the TagRFP excitation and the emission ...their excitation and emission spectra, but a good rule of thumb is that both the peak excitation wavelengths...substantial overlap between the excitation and emission filters and the excitation and emission spectra of the... requires multiple fluorescent proteins whose excitation and emission spectra differ sufficiently for ...microscope optics, you will want to compare the excitation and emission spectra of your protein with the... the protein, so that the protein is well excited by your microscope and the fluorescence emission of ...mCherry spectrum is shown in the lighter colors; excitation spectra are blue and emission spectra are red...

  18. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ...Considering citations to all articles, Anurag Acharya and colleagues report, the percentage of citations to articles...to 1975 and is based on the average number of citations for recent articles. Of course, a publication ...measure scientific impact. Even if we stick to citation counts for a moment, it’s clear that articles ...Non-Elite Journals” found that the proportion of highly cited articles appearing in lower-prestige journals has...articles in non-elite journals went from 27% of all citations in 1995 to 47% in 2013 – yes, almost half.   The...Accessibility is surely also behind the increase in citations for older publications. (As an interesting aside...aside, Nature recently reported that the most cited work in history is a 1951 description of an assay to...

  19. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...photostable. It also has narrow excitation and emission peaks (max excitation at 493 nm and max emission at...fluorophores that excite in the violet range. For example, mWasabi can be used with Sapphire (excitation maximum...We are excited about our new partnership with Allele Biotechnology which allows researchers to deposit...than the most commonly used GFPs and YFPs. Its excitation maxima is at 506 nm and its emission maxima is... replacements relative to CFP484. Because the excitation and emission maxima were red-shifted (462 nm ...researchers recommend using mTFP1 with a 445/30 nm excitation filter, a 470 nm beamsplitter, and a 495/30 nm... CFP set. mTFP1 can be used as a FRET donor to mCitrine and mOrange or for live cell imaging of fusions...

  20. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ... cloning capacity, decreased  potential for the generation of RCA and reduced immunogenicity. In addition...increased cloning capacity (up to 36 kb!), long-term transgene expression and negligible toxicity. However, their...transgene expression (Interestingly, the strong immunogenicity of AdVs is what makes them ideal candidates... Ad vectors (helper-dependent (HD-Ad), or high-capacity (HC-Ad__ are devoid of viral genes and therefore...such as HEK293 cells. 1st generation AdV cloning capacity is limited to 8.2 kb, and in vivo transgene expression...2nd generation AdVs, thereby increasing cloning capacity to ~12 Kb. The ever popular pAdEasy system belongs...to this category. 3rd Gen AdV (Gutless or high capacity AdV) are devoid of all viral coding sequences ...
Showing: 61 - 80 of 568 results