We narrowed to 340 results for: CO
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TypeBlog Post...et al, 2012, Bindels et al, 2017). The plasmid for co-expressing mTurquoise2 with mVenus as a reference...this data. The practical brightness assay (employing co-expressing fluorescent proteins or using endogenous...
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Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog Post...postdoctoral researcher at the Noble Research Institute, co-instructed the ASPB workshop and authored a three-part...Global Community Bio Summit at the MIT Media Lab. As a co-founder of a community lab, I realized that not all... -
Antibodies 101: Isotypes
TypeBlog Post... with its variable chain domain. B cells start by co-expressing IgM and IgD antibodies, but later in an...recombination of the antibody gene locus. B cells start by co-expressing IgM and IgD isotypes antibodies which ... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post...number of new platforms (such as openUC2, which I am a co-founder of), that aim to support the community in...the Heintzmann Lab at the Leibniz IPHT Jena. He is co-founder of the open-hardware startup “openUC2” and... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...ClonTracer library originally came about as Bhang and co-workers were trying to develop a way to quantify ...ClonTracer to study drug resistance Although Bhang and co-workers started using the tool to look at a variety... -
Working with Nuclear Receptors
TypeBlog Post...steady-state complexes with secondary coregulator (or co-coregulator) partners. The coregulator ‘core’ complex...group of proteins, whereas the more loosely bound co-coregulators associate with the core complex in a... -
Antibodies 101: Isotypes
TypeBlog Post... with its variable chain domain. B cells start by co-expressing IgM and IgD antibodies, but later in an...recombination of the antibody gene locus. B cells start by co-expressing IgM and IgD isotypes antibodies which ... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post...number of new platforms (such as openUC2, which I am a co-founder of), that aim to support the community in...the Heintzmann Lab at the Leibniz IPHT Jena. He is co-founder of the open-hardware startup “openUC2” and... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog Post...postdoctoral researcher at the Noble Research Institute, co-instructed the ASPB workshop and authored a three-part...Global Community Bio Summit at the MIT Media Lab. As a co-founder of a community lab, I realized that not all... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...et al, 2012, Bindels et al, 2017). The plasmid for co-expressing mTurquoise2 with mVenus as a reference...this data. The practical brightness assay (employing co-expressing fluorescent proteins or using endogenous... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...ClonTracer library originally came about as Bhang and co-workers were trying to develop a way to quantify ...ClonTracer to study drug resistance Although Bhang and co-workers started using the tool to look at a variety... -
Working with Nuclear Receptors
TypeBlog Post...steady-state complexes with secondary coregulator (or co-coregulator) partners. The coregulator ‘core’ complex...group of proteins, whereas the more loosely bound co-coregulators associate with the core complex in a... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...independently packaged into an AAV. When a cell is co-infected with both part A and part B AAVs, the fragments...of these variables influence whether cells will be co-transduced with multiple copies of both vectors. ...and “part B” of the transgene. These two AAVs are co-transduced in a single step and serve as donors for... -
Plasmids 101: Repressible Promoters
TypeBlog Post...promoter sequence and transcription is ON. Once a co-repressor protein binds the repressor protein, the...promoter is less leaky than UAS in the basal state, and co-expression of the inducer QF can increase QUAS driven...human HeLa cells as compared to basal expression. Co-expression of repressor QS along with QF leads to... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...independently packaged into an AAV. When a cell is co-infected with both part A and part B AAVs, the fragments...of these variables influence whether cells will be co-transduced with multiple copies of both vectors. ...and “part B” of the transgene. These two AAVs are co-transduced in a single step and serve as donors for... -
Plasmids 101: Repressible Promoters
TypeBlog Post...promoter sequence and transcription is ON. Once a co-repressor protein binds the repressor protein, the...promoter is less leaky than UAS in the basal state, and co-expression of the inducer QF can increase QUAS driven...human HeLa cells as compared to basal expression. Co-expression of repressor QS along with QF leads to... -
Participate in the Pets of Addgene Scavenger Hunt
TypeBlog Post...means spending more time with our pets. These new “co-workers” have made their way onto our Slack workspace... -
Participate in the Pets of Addgene Scavenger Hunt
TypeBlog Post...means spending more time with our pets. These new “co-workers” have made their way onto our Slack workspace... -
CRISPR References and Information
TypeCollection...neuronal co-injection marker); pCFJ104 (body wall muscle co-injection marker); pCFJ90 (pharyngeal co-injection... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post...viruses to express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally high enough...packaged in them and use elements such as IRES or 2A to co-express them from one promoter. Specificity The ...