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We narrowed to 184 results for: CO

Showing: 61 - 80 of 184 results

  1. Plasmids 101: Protein tags

    Type
    Blog Post
    ...1.1 Affinity Frequently used for western blots, IP, co-IP, IF, flow -cytometry; can occassionally interfere...1.2 Affinity Frequently used for western blots, IP, co-IP, IF, flow -cytometry, but rarely used for purification...

  2. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... tissue culture plate. Incubate cells at 37°C, 5% CO 2 overnight. Although cells should regularly be passaged...cells from the plate. i. Incubate cells at 37°C, 5% CO 2 for 12-15 hours. Day 3: j. In the morning, change... transfected cells. k. Incubate cells at 37°C, 5% CO 2 for 24 hours. Day 4: l. Harvest media from cells...antibiotics to the cells and incubate at 37°C, 5% CO 2 for 24 hours. Day 5: n. Harvest media from cells...target cells in ten 6 cm plates and grow at 37°C, 5% CO 2 overnight. Day 2: b. The target cells should be...1: a. Plate target cells and incubate at 37°C, 5% CO 2 overnight. Day 2: b. Target cells should be approximately...your target cell line. d. Incubate cells at 37°C, 5% CO 2 overnight. Day 3: e. Change to fresh media 24 hours...

  3. TALEN Guide

    Type
    Collection
    ... ligation protocol to assemble arrays. Dr. Joung, co-founder of the Zinc Finger Consortium with Dr. Voytas...

  5. p53 Pathway

    Type
    Collection
    ...binding activity, and allow it to bind transcriptional co-activators and modulate transcription. The list of...

  7. General Transfection

    Type
    Protocol
    ...pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...tissue culture plates. Incubate the cells at 37 °C, 5% CO 2 for ~20 h. Gently aspirate media, add 10 mL fresh...

  10. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...enhances RNA-guided methylcytosine editing by TET1 by co-delivering TET1 and protein factors for linking methylcytosine...

  12. CRISPR Guide

    Type
    Collection
    ... 8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a ...genes in mammalian cells include: SunTag system — co-expression of epitope-tagged dCas9 and antibody-activator...two-component system (Figure 9C) SAM activators — co-expression of dCas9-VP64 with a modified scaffold...non-repetitive genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome...dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase. Whatever the chosen...other Cas enzymes, the Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex...

  18. Lentivirus Production

    Type
    Protocol
    ...pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...tissue culture plates. Incubate the cells at 37 °C, 5% CO 2 for ~20 h. Prepare a mixture of the three transfection...

  20. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...cre recombinase (either from a mouse line or viral co-injection), the opsin is inverted into the correct...opsin expression, extent of viral spread, and with co-localization immunofluorescence, cell-type specificity...
Showing: 61 - 80 of 184 results