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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 636 results for: ESP

Showing: 61 - 80 of 636 results

  1. DNA Quantification

    Type
    Protocol
    ...Repeat for each sample. Notes: Keep in mind that despite the accuracy of the NanoDrop, if two consecutive...

  4. Gibson Assembly Protocol

    Type
    Protocol
    ...stitch” together several oligos. This technique is especially useful for introducing promoters, terminators...

  11. Pouring LB Agar Plates

    Type
    Protocol
    ...plates with colored marker - particular colors correspond to particular antibiotics. Position the flame...

  13. Western Blot

    Type
    Protocol
    ...the electrical contacts are aligned with the corresponding electrical contacts on the blotting surface ...

  14. Lentivirus ddPCR Titration

    Type
    Protocol
    ... probes targeting integrated copies of the Rev response element (RRE) in the target cells but can be modified...

  15. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...fluorescent proteins respond to magnetic fields?” and “What are magnetoresponsive fluorescent proteins...developing a new fluorescent protein tool that responds strongly to magnetic fields (Frank Hayward et ...the tool, and develop new applications for magnetoresponsive proteins. Motivated by curiosity Although...Maria dreamed about the possibilities of a magnetoresponsive fluorescent protein. Controlling an optogenetic...variety of research on whether and how organisms respond to magnetic fields (Karki et al., 2021; Kattnig...encouraged by previous reports that described magnetic responses of cryptochrome proteins or organic fluorophores...conditions. So, they decided to search for a magnetic response among other proteins that might serve as a starting...

  16. All in a Twist: dsRNA

    Type
    Blog Post
    ... The foreign dsRNA activates the innate immune response machinery of the host. This pathway has been ...into a healthy individual to provoke an immune response and then, hopefully, immunity to that virus. This...balancing antiviral benefits with the inflammatory response associated with the introduction of dsRNA. Synthetic...multiple receptors in place to sense dsRNA and respond accordingly. While not perfect, these sensing systems...distinguish between RNA of cellular vs. viral origin and respond appropriately. When these sensing systems fail,... cellular consequences arise. dsRNA An immune response is activated if and when dsRNA levels reach a ...thus do not typically activate an inflammatory response. However, if dsRNA sensors are mutated or mechanisms...

  17. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...kinase that phosphorylates a response regulator (RR). TCS-induced responses are varied; one outcome is ...system to respond. In far-red light or dark conditions, the chromophore activates the response regulator...far-red/red light-responsive system is spread across three plasmids, with the response regulator encoded...Tabor have been working on making E. coli light responsive since 2005, when Tabor was working in Voigt's...gene expression controls. Most cell types do not respond to low-intensity light, so off-target effects should... should be limited. Phytochromes are light-responsive systems found in some bacteria, but not E. coli....and an obligate chromophore allows the system to respond to various light inputs. In far-red light or dark...

  18. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...Try NgAgo for Yourself On the other hand, the widespread success of CRISPR-Cas9 across multiple model ... questions about the NgAgo protocols used by respondents (transfection method, amount of each component...of August 1, 2016, a total of 165 researchers responded to the survey. When asked if they could detect...indication of gene disruption ability) with NgAgo, one respondent out of 88 confirmed successful indel formation...formation at a target site. Also, 41 respondents attempted to use the technique for epitope tagging but only...favorite model system. The vast majority of the respondents (70%) used a lipofectamine-based method to deliver...Survey The take-home message While most of the respondents have been struggling to achieve genome editing...

  19. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ... to develop a spatiotemporal view of an immune response, but the study of these dynamics was still laborious...measurements of cellular motility, interactions, and response dynamics. In the past 15 years efforts have been...understand T cell initiation in the adaptive immune response. It was known that T cells were able to extravasate... monocytes patrol blood vessels during immune responses (Auffray et al. 2007, Finsterbusch et al. 2016...specificities have to be taken into consideration especially in the use of fluorescent proteins to track cellular...their degradation within labelled cells limits the timespan available for tracking certain cell types. Another...dendritic cells within the skin and lymph node respectively. The permanent labelling of cells using cre-...

  20. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...modulators, shaping the immune response by driving particular cells or responses. And there are still antibody...in helping antibodies drive an effective immune response against a pathogen. The Fc domain creates a link...the immune system, including the innate immune response, by binding to Fc receptors on other immune cells...! Need a quick review of the different immune responses? We got you! You can also review antibodies in...FcγRIIB1 and FcγRIIB, inhibit) specific immune responses. These receptors are specific to isotype class...different isotypes drive different types of immune responses. Handily, the receptors are named after the isotypes... Figure 1: Fc effector functions in an immune response to a viral infection a) antibody-dependent complement...
Showing: 61 - 80 of 636 results