...the volume of homogenized cell solution that corresponds to 50 cells: Volume of homogenized cell solution... be sure to look thoroughly within each well, especially in the corner of the well as cells tend to settle...

...Repeat for each sample. Notes: Keep in mind that despite the accuracy of the NanoDrop, if two consecutive...

...anyone else associated with Addgene, can be held responsible for your use of the information contained in ...

...antigen to demonstrate the expected antibody dose response....

...anyone else associated with Addgene, can be held responsible for your use of the information contained in ...

...higher transformation efficiencies with less DNA, especially when using highly competent cells. If you used...

...instructions). Note: For many ligation reactions, especially if using "high concentration" ligase, 5min at...

...stitch” together several oligos. This technique is especially useful for introducing promoters, terminators...

...each QuickSeal tube. The first 3 mL collected corresponds to the 60% phase and can be discarded. The fractions...

... temperature around 70°C and is the molecule responsible for driving the DNA synthesis. Sterile dH 2 O...

... the transgene may have reduced growth rates, especially if that transgene is toxic. Eventually, the rapidly...

...plates with colored marker - particular colors correspond to particular antibiotics. Position the flame...

...look as you expected. When cloning by PCR, it is especially important to run the product on a gel. This allows...

...the electrical contacts are aligned with the corresponding electrical contacts on the blotting surface ...

... probes targeting integrated copies of the Rev response element (RRE) in the target cells but can be modified...

...fluorescent proteins respond to magnetic fields?” and “What are magnetoresponsive fluorescent proteins...developing a new fluorescent protein tool that responds strongly to magnetic fields (Frank Hayward et ...the tool, and develop new applications for magnetoresponsive proteins. Motivated by curiosity Although...Maria dreamed about the possibilities of a magnetoresponsive fluorescent protein. Controlling an optogenetic...variety of research on whether and how organisms respond to magnetic fields (Karki et al., 2021; Kattnig...encouraged by previous reports that described magnetic responses of cryptochrome proteins or organic fluorophores...conditions. So, they decided to search for a magnetic response among other proteins that might serve as a starting...

... The foreign dsRNA activates the innate immune response machinery of the host. This pathway has been ...into a healthy individual to provoke an immune response and then, hopefully, immunity to that virus. This...balancing antiviral benefits with the inflammatory response associated with the introduction of dsRNA. Synthetic...multiple receptors in place to sense dsRNA and respond accordingly. While not perfect, these sensing systems...distinguish between RNA of cellular vs. viral origin and respond appropriately. When these sensing systems fail,... cellular consequences arise. dsRNA An immune response is activated if and when dsRNA levels reach a ...thus do not typically activate an inflammatory response. However, if dsRNA sensors are mutated or mechanisms...

...kinase that phosphorylates a response regulator (RR). TCS-induced responses are varied; one outcome is ...system to respond. In far-red light or dark conditions, the chromophore activates the response regulator...far-red/red light-responsive system is spread across three plasmids, with the response regulator encoded...Tabor have been working on making E. coli light responsive since 2005, when Tabor was working in Voigt's...gene expression controls. Most cell types do not respond to low-intensity light, so off-target effects should... should be limited. Phytochromes are light-responsive systems found in some bacteria, but not E. coli....and an obligate chromophore allows the system to respond to various light inputs. In far-red light or dark...

...Try NgAgo for Yourself On the other hand, the widespread success of CRISPR-Cas9 across multiple model ... questions about the NgAgo protocols used by respondents (transfection method, amount of each component...of August 1, 2016, a total of 165 researchers responded to the survey. When asked if they could detect...indication of gene disruption ability) with NgAgo, one respondent out of 88 confirmed successful indel formation...formation at a target site. Also, 41 respondents attempted to use the technique for epitope tagging but only...favorite model system. The vast majority of the respondents (70%) used a lipofectamine-based method to deliver...Survey The take-home message While most of the respondents have been struggling to achieve genome editing...

...modulators, shaping the immune response by driving particular cells or responses. And there are still antibody...in helping antibodies drive an effective immune response against a pathogen. The Fc domain creates a link...the immune system, including the innate immune response, by binding to Fc receptors on other immune cells...! Need a quick review of the different immune responses? We got you! You can also review antibodies in...FcγRIIB1 and FcγRIIB, inhibit) specific immune responses. These receptors are specific to isotype class...different isotypes drive different types of immune responses. Handily, the receptors are named after the isotypes... Figure 1: Fc effector functions in an immune response to a viral infection a) antibody-dependent complement...