...mutants gain new powers: news from the mutant p53 field. Brosh R, Rotter V. Nat Rev Cancer. 2009 Oct;9(10...

...opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert adenine...

...inventory below. Lentiviruses are distributed as purified viral particles in solution that are suitable ...

...Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed ...

...opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert adenine...

...PubMed . Promoter-associated proteins of EPAS1 identified by enChIP-MS - A putative role of HDX as a negative...

...plasmids called "transfer plasmids," which can be modified to encode a wide range of gene products. The main...

... or Inserts Several mammalian genes have been identified as having a key role in coronavirus infection...

...Optogenetics Guide page for more information about this field. Use the table and filters below to find ready-to-use...

...payloads to be expressed in cells based on a doubly-specified combination of genetic and/or anatomical-defined...

... to it, and, later, cleave those tags from the purified protein. Protein purification can be challenging...

...Cortactin-pmCherryC1 Actin Filaments Cortactin mCherry Christien Merrifield 36201 pLifeAct-mTurquoise2 Actin Filaments LifeAct...

...Christien Merrifield 27700 Hip1R-tDimerRFP-N1 Hip1R RFP CMV Parkinson's Christien Merrifield 28195 tdp43...Izaurralde 148689 pT7-EGFP-C1-HsGIGYF2_1-180-LFIENtoDDDAF_AF GIGYF2 GFP CMV Parkinson's Elisa Izaurralde...

...Calcium Indicators to Study Astrocyte Physiology: A Field Guide. Front Cell Neurosci. 2021 Jun 11;15:690147...

...individual’s work in their field and the impact of the individual’s past work on the field. NIW requirements stress...in the field. This criterion is best proven through letters of support from experts in the field. You must... of the work of those in your field. Original contributions to the field that have had a major impact....specific academic field, (2) that you have three 3 years of research experience in the field, (3) that you...academic field Evidence of original scientific or scholarly research contributions in the field Evidence...expertise and impact on your field from the point of view of experts in your field. Please remember, this is...research role could be the point of entry into that field. In this post, we discuss the following paths to...

...protein tool that responds strongly to magnetic fields (Frank Hayward et al., 2024). They deposited their... you’ve ever had an MRI, you know that magnetic fields can pass right through our tissues unimpeded. Andrew...on whether and how organisms respond to magnetic fields (Karki et al., 2021; Kattnig et al., 2016), and...Wheeler et al., 2016; Duret et al., 2019). But the field has sometimes seen controversy, and the interactions...of organic molecules like proteins with magnetic fields generally appears to be weak — if they exist at.... Gray regions indicate times when the magnetic field is on. Image reproduced from Frank Hayward et al...magnetoresponsive system that could be expressed and purified. Although a ~1% change in signal might not make...

...fused to epigenetic modifiers enabled epigenetic modifications at a user-specified locus. Researchers...epigenetic modifiers available from Addgene. For most of these constructs, catalytically dead modifiers are ...this purpose. Epigenetics began as a correlative field in which covalent modifications to DNA or histones...fusions between Cas proteins and the epigenetic modifier could be targeted to specific DNA sequences. Non-editing...catalytically dead dCas9 fused to a variety of epigenetic modifiers to specific loci without inducing double strand...increases levels of H3K27ac histone modification at specified loci. Charles Gersbach’s lab has deposited mammalian...transcription. A lentiviral vector with the same modifier, Fuw-dCas9-Tet1CD, is available from Rudolf Jaenisch...

...entire kit; a parallel analysis that successfully identified new, highly specific agonists for orphan GPCRs...Densmore’s lab at Boston University have greatly simplified the process of generating multiple E. coli gene...multiple screens in different human cell models and identified ~4-5-fold more fitness-related genes than have...across multiple species Recent discoveries in the fields of genome engineering and synthetic biology have...vectors that allows one to use the MAGE method in unmodified bacterial species (4). This set of plasmids (...oligonucleotide integration, allowing MAGE in otherwise unmodified bacterial strains (5). MutL is highly conserved...modifications has been to use inhibitors of epigenetic modifiers like DNA methyltransferases or histone deacetylases...

...exactly what aspect of the field you plan to review. If it’s a well-researched field, you may need to get specific...moving - remember you're becoming an expert in your field and are the perfect person to be writing the review... of exposing you to relevant researchers in the field. Finally, you can sound credible and cool when you...because, as I mentioned, I wasn’t an expert in the field. To find reviews, I just searched online and found...these articles to get a sense of the themes in the field and to learn what people cared about. I also used...to read. Once I had an idea of the themes in the field, I searched for recent research papers on these ...articles from the active/well-known researchers in the field. I made sure to find information from genome-wide...

...microscopy techniques   Fluorescent Widefield Microscopy Fluorescent Widefield Microscopy is the most common...to solve two drawbacks of widefield and confocal microscopy. First, widefield and confocal microscopy project... limiting photobleaching. Similar to widefield, the entire field of view is excited at one time and captured...objective evenly illuminating the entire (wide) field of view. Fluorescent light traveling back towards...samples are thin enough to sit within the depth of field of the objective (the distance in the axial dimension...these experiments are performed on a fluorescent widefield microscope. Here, excitation light is projected...projected from the objective so that the entire field of view is bathed with an even illumination. The emitted...