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We narrowed to 395 results for: ICL

Showing: 61 - 80 of 395 results
  1. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...here.        AAV delivery of split base editors Article contributed by Jennifer Tsang AAVs are a great ...fluorescent genetically encoded voltage indicator Article contributed by Shreya Vedantam Genetically encoded...: 31685893. A MoClo toolkit for cyanobacteria Article contributed by Andrew Hempstead The Modular Cloning...optogenetic tool for precision in cell lineage tracing Article contributed by Erin Sanders CreLite, designed by...
  2. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...Optogenetic control of CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley  Listen to the anti-...using the fluorescent voltage indicator Archon Article contributed by Shreya Vedantam  Listen to the voltage...studies easier with a 2in1 vector cloning system Article contributed by Michelle Cronin  Listen to the 2in1...tools for photoswitchable proteins cPYP and AsLOV2 Article contributed by Angela Abitua  Listen to the photoswitchable...
  3. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...Didier Trono's lab webpage explains how lentiviral particles are produced by co-transfecting three essential...envelope glycoprotein will be expressed on virus particles? A5: This depends on the envelope plasmid you ...common due to its broad host range, and increased particle stability; however, the protein is cytotoxic precluding...target cells, but cannot produce any new viral particles after the initial infection. SIN is shorthand ...all the components necessary to produce viral particles. The components are divided as follows: Transfer...Meghan Rego contributed to the writing of this article. Additional Resources: Didier Trono Lab: Lentivectors...
  4. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...transfected into target cells which produce lentiviral particles.   Why pseudotype? Lentiviruses and retroviruses...4 lab. For more details, check out this review article published in Reviews in Medical Virology (Li et... proteins have been pseudotyped on lentiviral particles. Rabies glycoprotein The rabies glycoprotein targets...suit your needs. Check out some of these review articles to find tables and information on different viral...Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization...Jr (2000) VSV-G Pseudotyped Lentiviral Vector Particles Produced in Human Cells Are Inactivated by Human...
  5. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ... Chromatin research articles with plasmids available from Addgene Many articles reporting chromatin-related...plasmids from hundreds of articles in our collection; the following articles are just a small cross-section...jumping off point. The following lists of PIs, articles, genes, and blog posts are not comprehensive, ...
  6. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...very confusing for scientists writing research articles. What should you take into account when writing...funders of your research may stipulate that your article must be published in an open access journal or ...whether they agree with the conclusions of the article. These initiatives are another way in which journals...report are permanently attached to the published article. In addition, authors and readers are able to comment..., the authors can publish a new version of the article, while the first version and the referee reports...
  7. Summer SciComm: Preprints

    Type
    Blog Post
    ...from formal mechanisms like publishing a research article, presenting a conference poster or talk, or submitting...avoid many of these issues, by sharing their work publicly and immediately once the manuscript is drafted...somewhat meaningless) practice of listing “Submitted” articles in their CV or grant applications. A number of...source or were deposited when the now-published article was a preprint. Depositing plasmids that were used... as sharing those plasmids alongside a journal article, but it makes the research materials available ...
  8. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ... viral shRNA expression vector and the vector particles are delivered into the brain region of interest...vectors are used for intracerebral injections. AAV particles are smaller and may be preferable if wider diffusion...via injection into the liquor of the cerebral ventricles (ICV, intracerebroventricular) to minimize tissue... titer: The concentration of infectious viral particles must be high enough to maximize possible outcomes...injection speed has to be slow to allow the viral particles to diffuse away from the injection site, and also...
  9. Synthetic Biology - Browse Plasmids

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...
  10. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...during mitosis, support transport of organelles and vesicles, and more. Although several strategies are available... demonstrated its use to probe cell mechanics, vesicle transport, and cell division, but given the long...Aljiboury et al., 2023). Rab family proteins support vesicle trafficking and organelle dynamics, but with dozens...Figure 3: Optogenetic inhibition of Rab protein vesicle trafficking via CRY2-CIB1 clustering. Image adapted... the plasma membrane or lysosomes (the earlier article also provided CIB constructs for targeting Golgi...
  11. Synthetic Biology - Algal

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...
  12. Synthetic Biology - Bacterial

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...
  13. Synthetic Biology - Fungal

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...
  14. Synthetic Biology - Mammalian

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...
  15. Synthetic Biology - Plant

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...
  16. Synthetic Biology - Worm

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...
  17. Synthetic Biology - Yeast

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...
  18. Synthetic Biology - Strains

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...
  19. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...repository through our hot plasmids articles. These brief articles provide a synopsis of a plasmid or ...functions and applications. We hope that these articles make it easier for you to find and use the plasmids...glia, have been successfully targeted via viral particles, an effective approach to target specific neuronal...HF-BE3 protein for delivery in ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse...localize TRF2 to telomeres. The plasmids used in this article have been deposited with Addgene and include MYC...
Showing: 61 - 80 of 395 results