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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 395 results for: ICL

Showing: 61 - 80 of 395 results

  1. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...here.  Human whole genome sgRNA iBAR library Article contributed by Susanna Bachle Figure 1: Initial...Sparse cell labeling using recombinase competition Article contributed by Aliyah Weinstein Sparse Predictive...GFP reporter for calcineurin activity in yeast Article contributed by Leah Schwiesow Calcineurin is a ...TALE-fluorescent protein fusions as a DNA staining dye Article contributed by Jennifer Tsang Figure 4: Double...

  2. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...   Articles with and without plasmids at Addgene To find out whether scientific articles associated...bibliographic information and uses its vast network of articles to track the total number of citations the paper... of which had plasmids deposited with Addgene. Articles with Addgene plasmids get more citations So what...estimates based on the journal that published the articles.  That is, we re-estimate the citations to the...weights. Table 1: Journal Weighting Example Article Number (A) Year Published (B) Number of Citations...

  3. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...   A retroviral mouse CRISPR knockout library Article contributed by Alycia Bittner CRISPR pooled libraries...reporter for measuring ApoB bound lipoproteins Article contributed by Leah Schwiesow A leading cause of...transcription for CRISPR activation in bacteria Article contributed by Will Arnold Baojun Wang’s lab recently...Genetically encoded voltage indicators for use in worms Article contributed by Brook Pyhtila Genetically encoded...

  4. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...to as hosts) via viral particles, the functional infection unit. Each particle has a viral envelope, which...the target cell’s membrane, allowing the viral particle access into the cell. The viral envelope and its...cargo – its own genome.   Fig. 1: Viral particle infection into a host cell. Image courtesy of ... measures, like replication incompetent viral particles, are required. We will be covering these safety...localization signals which can traffic their viral particles inside the nucleus, without the need for envelope...

  5. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...efficient RNAi construct for C. elegans gene knockdown Article contributed by Alyssa Cecchetelli  Listen to the... proximity labeling with TurboID and miniTurbo Article contributed by Eric Perkins  Listen to the TurboID.... Changing the way we detect viral outbreaks Article contributed by Izzy Mueller  Listen to the viral...collection reveals novel peptide ligand motifs Article contributed by Michael Lemieux  Listen to the SH3...

  6. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...Optogenetic control of CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley  Listen to the anti-...using the fluorescent voltage indicator Archon Article contributed by Shreya Vedantam  Listen to the voltage...studies easier with a 2in1 vector cloning system Article contributed by Michelle Cronin  Listen to the 2in1...tools for photoswitchable proteins cPYP and AsLOV2 Article contributed by Angela Abitua  Listen to the photoswitchable...

  7. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...here.        AAV delivery of split base editors Article contributed by Jennifer Tsang AAVs are a great ...fluorescent genetically encoded voltage indicator Article contributed by Shreya Vedantam Genetically encoded...: 31685893. A MoClo toolkit for cyanobacteria Article contributed by Andrew Hempstead The Modular Cloning...optogenetic tool for precision in cell lineage tracing Article contributed by Erin Sanders CreLite, designed by...

  8. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...Didier Trono's lab webpage explains how lentiviral particles are produced by co-transfecting three essential...envelope glycoprotein will be expressed on virus particles? A5: This depends on the envelope plasmid you ...common due to its broad host range, and increased particle stability; however, the protein is cytotoxic precluding...target cells, but cannot produce any new viral particles after the initial infection. SIN is shorthand ...all the components necessary to produce viral particles. The components are divided as follows: Transfer...Meghan Rego contributed to the writing of this article. Additional Resources: Didier Trono Lab: Lentivectors...

  9. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...transfected into target cells which produce lentiviral particles.   Why pseudotype? Lentiviruses and retroviruses...4 lab. For more details, check out this review article published in Reviews in Medical Virology (Li et... proteins have been pseudotyped on lentiviral particles. Rabies glycoprotein The rabies glycoprotein targets...suit your needs. Check out some of these review articles to find tables and information on different viral...Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization...Jr (2000) VSV-G Pseudotyped Lentiviral Vector Particles Produced in Human Cells Are Inactivated by Human...

  10. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...lab used SNAP-tag to decorate synthetic viral particles with BG-linked antibodies to drive cell type-specific...intracellular targets. Nature Communications, 7(1), Article 1. https://doi.org/10.1038/ncomms10778 Erdmann,...molecules in vivo. Nature Biotechnology, 21(1), Article 1. https://doi.org/10.1038/nbt765 Lavis, L. (2016...A flexible chemical tag. Nature Methods, 2(4), Article 4. https://doi.org/10.1038/nmeth749 Strebinger,...envelope design. Nature Communications, 14(1), Article 1. https://doi.org/10.1038/s41467-023-40788-8 Ulrichs...capping protein. Nature Communications, 14(1), Article 1. https://doi.org/10.1038/s41467-023-39655-3 Wilhelm...

  11. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ... Chromatin research articles with plasmids available from Addgene Many articles reporting chromatin-related...plasmids from hundreds of articles in our collection; the following articles are just a small cross-section...jumping off point. The following lists of PIs, articles, genes, and blog posts are not comprehensive, ...

  12. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...very confusing for scientists writing research articles. What should you take into account when writing...funders of your research may stipulate that your article must be published in an open access journal or ...whether they agree with the conclusions of the article. These initiatives are another way in which journals...report are permanently attached to the published article. In addition, authors and readers are able to comment..., the authors can publish a new version of the article, while the first version and the referee reports...

  13. Summer SciComm: Preprints

    Type
    Blog Post
    ...from formal mechanisms like publishing a research article, presenting a conference poster or talk, or submitting...avoid many of these issues, by sharing their work publicly and immediately once the manuscript is drafted...somewhat meaningless) practice of listing “Submitted” articles in their CV or grant applications. A number of...source or were deposited when the now-published article was a preprint. Depositing plasmids that were used... as sharing those plasmids alongside a journal article, but it makes the research materials available ...

  14. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ... viral shRNA expression vector and the vector particles are delivered into the brain region of interest...vectors are used for intracerebral injections. AAV particles are smaller and may be preferable if wider diffusion...via injection into the liquor of the cerebral ventricles (ICV, intracerebroventricular) to minimize tissue... titer: The concentration of infectious viral particles must be high enough to maximize possible outcomes...injection speed has to be slow to allow the viral particles to diffuse away from the injection site, and also...

  15. Synthetic Biology - Browse Plasmids

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

  16. Synthetic Biology - Algal

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

  17. Synthetic Biology - Bacterial

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

  18. Synthetic Biology - Fungal

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

  19. Synthetic Biology - Mammalian

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

  20. Synthetic Biology - Plant

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...
Showing: 61 - 80 of 395 results