We narrowed to 642 results for: PPL

...column in a new 50 mL LoBind collection tube. Slowly apply the sample to the center of the compact resin bed...exchange and concentration with an Amicon column Apply 15 mL of PBS to the reservoir of a Amicon Ultra-...

...Spectrophotometer Heat block Mini gel tank chamber Power supply iBlot 2 Gel Transfer Device Roller Spatula Platform...tank and plug the electrode cords into the power supply. Run the gel at 100 V for 10–15 min or until the...

.... Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting Viviana Gradinaru 163505-PHPeB...

...EYFP as payloads). This approach has been broadly applied using multiple recombinase-expression strategies...

...Water, Hyclone, SH30538.02 GeneAmp 10X PCR Buffer, Applied Biosystems, N8080129 ddPCR Supermix for Probes ... the BSC to be used for droplet generation are supplied with sufficient pipette tips and reagent reservoirs...

... not affect the quality of RNA or downstream applications. To improve yield of RNA, instead of incubating...

...Davidson 54924 mApple-MAPTau-C-10 MAPT mApple CMV Parkinson's, FTD Michael Davidson 54925 mApple-MAPTau-N-10...-10 MAPT mApple CMV Parkinson's, FTD Michael Davidson 55077 mCherry-MAPTau-C-10 MAPT mCherry CMV Parkinson's...

...BiFC studies. ID Plasmid Fluorescent Protein Application PI 14030 14031 pCyPet-His pYPet-His CyPet (donor...

...extensively validated for neuroscience research applications. A substantial number of these have been cloned...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...

...according to speed and time (and temperature, if applicable) listed in your protocol. Pro-Tip Spin speed ...

... these cells can still be used in downstream applications. Safety Warnings Lentivirus is generally considered...

...working in the lab. Do not eat, drink, chew gum, or apply makeup in the laboratory. Before you start your ...

... take up the DNA, an electro-magnetic field is applied to the cell/DNA mixture to induce membrane permeability...

...as methanol or acetone may be better for some applications. Remove the paraformaldehyde and follow your...

...lentivirus can be used for a variety of downstream applications such as stable-cell line generation. Workflow...

...the plasmid DNA. Either spin to pellet the DNA or apply the solution to a column that will bind the now ...

...lower dilutions depending on your downstream applications. If you’ve titered your virus beforehand, you...

...sufficient quantities of library for experimental applications. Repeated amplifications should be avoided as...

...Safety Cabinet (BSC). Make sure that the BSC is supplied with sufficient pipette tips. Pre-warm the 96-...

... various RNA molecules - such as MS2 stem loops, PPL stem loops, the Spinach2 aptamer, and more - to sequences...potential applications of CRISPR/Cas9 in genome-engineering. One particularly attractive application involves... system widely accessible for many potential applications. Polstein LR & Gersbach CA, Nat Chem Biol ...combinations of states. Each of the integrases is supplied on its own expression plasmid or in various combinations...combinations, while the cognate att sites are supplied as individual reporter constructs or as the fully...amenable to both high and low throughput screening applications. The system can be readily modified to use different...cloning platform for imaging and flow cytometry applications Designing customized fluorescent reporters can...