We narrowed to 117 results for: REP
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CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
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Adeno-associated virus (AAV) Guide
TypeGuide...reading frames, Rep and Cap, flanked by two 145 bp inverted terminal repeats (ITRs). The Rep region encodes...Packaging plasmid (also known as Rep/Cap plasmid) — containing the Rep and Cap regions necessary for the...to specific Rep-binding elements present in the AAV ITRs and the AAVS1 site. Since the Rep region is removed...Repeat, contains a Rep binding element (RBE) that serves as a binding site for Rep proteins essential ...required but often included. Packaging plasmid Rep in trans The rep gene encodes four proteins that are required...Recombinant AAV constructs are designed by replacing the Rep and Cap regions between the two ITRs with a gene ... -
Pooled Library Amplifications
TypeBlog Post...bp, plus a 20 bp barcode 400 ng Solid pCMV-Rep 78/68 SSM Library Amplification and Sequencing Protocol... -
Viral Vectors 101: Voltage Indicators
TypeBlog Post...fluorescent voltage sensor to detect neural activity. Sci Rep 9, 15878 (2019). doi: 10.1038/s41598-019-52370-8 ... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...mutagenesis in a diverse array of human cells. Sci Rep 4: . https://doi.org/10.1038/srep05105 Stephens CJ... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post... the SAM system differ in target preference. Sci Rep 9: . https://doi.org/10.1038/s41598-019-54179-x Tanenbaum... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...Degron Tagging with Short Homology Donors.” Cell Rep.15(1) (2016):210-8. PubMed PMID: 27052166. Kunzelmann... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...Mapping Multiprotein Complexes in Human Cells.” Cell Rep. 13: 621-633. PMID: 26456817. Find plasmids from... -
Antibody Plasmid Collection
TypeCollection...cloning and expression of recombinant antibodies. Sci Rep. 2014 Jul 30;4:5885. Andrew Beavil Cloning and mammalian...