Skip to main content

We narrowed to 754 results for: REV;

Showing: 61 - 80 of 754 results
  1. Chemogenetics Guide

    Type
    Guide
    ...Neuronal Biology. Physiol Rev, 98 (1), 391–418. https://doi.org/10.1152/physrev.00009.2017 PMID: 29351511...brain functions. Annu Rev Neurosci, 37 , 387–407. https://doi.org/10.1146/annurev-neuro-071013-014048 PMID...Written and reviewed by the Scientific Curation team at Addgene. Last Updated Content last reviewed on 4 March...alternatives for experiments. Perlapine has been previously used in human populations in Japan, making it...Bonci, A., & Michaelides, M. (2017). Chemogenetics revealed: DREADD occupancy and activation via converted...
  2. Tetracycline Inducible Expression

    Type
    Collection
    ...Written and reviewed by the Scientific Curation team at Addgene. Last Updated Content last reviewed on 20 June...most popular choices for rapid, efficient, and reversible control of gene expression in eukaryotes. To ...VP16 transcriptional activation domain); rtTA: reverse tetracycline-controlled transactivator (fusion ...for tetracycline-dependent repression led to a reverse Tet repressor, or rTetR, which specifically binds...the TRE in the presence of tetracycline. A new reverse tetracycline-controlled transactivator (rtTA) was...system, even though the DNA-binding profile is reversed (Figure 1). However, rtTA can induce much stronger...
  3. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...group. Reviewed by the Scientific Curation team at Addgene. Last Updated Content last reviewed on 12 August...red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902. https://doi.org/10.1371... fluorescent protein tagging in Saccharomyces cerevisiae. Yeast, 21 (8):661-70. https://doi.org/10.1002...selection markers for engineering in Saccharomyces cerevisiae. Microb Cell Fact, 12 :96. https://doi.org/10.1186...
  4. Caltech Systemic Capsids

    Type
    Collection
    ...140135 pAAV-EF1a-iCreV EF1a light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible...strains. **AAV1-X1 has not been tested in rats. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory...check out Addgene's blog on Systemic Capsids . To review performance data of each of these serotypes, please...light-inducible recombinase iDreV Zeng 140137 pAAV-EF1a-iFlpV EF1a light-inducible recombinase iFlpV Zeng 164450...
  5. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...2009. PubMed PMID: 19633652 Court, et al. Annu Rev Genet. 2002. PubMed PMID: 12429697 Lajoie, et al...this screen the authors were able to identify 7 previously unsuspected genes with known roles in ER-associated... fitness-related genes than have been found in previous screens of this type. They were also able to functionally...backward compatible with all TAL effector scaffolds previously constructed for the Golden Gate TALEN and TAL..., SnoopTag/SnoopCatcher, that can be used to irreversibly link desired proteins together via a spontaneous...isopeptide bond. When used in combination with their previously described SpyTag/SpyCatcher pair, multiple protein... industry, depend on the yeast, Saccharomyces cerevisiae, as a key host for the production of their products...
  6. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... vectors - PCR-based C-terminal tagging in S. cerevisiae pCS2FLAG - N- or C-terminal 2xFLAG...of proteins on the extracellular surface of S. cerevisiae cells His-tagged versions of pFastBac LIC vectors...Plasmids page pBF3060 - Metabolic engineering in S. cerevisiae TALENs Gene targeting TALEN kits - Construct ... and expression of bacterial gene clusters S. cerevisiae plasmids for multiple marker selection TRP1 Yeast...InvivoGen trademark. Return to top Content last reviewed 28 May 2025. Do you have suggestions for other...
  7. Zhang Lab CRISPR Page

    Type
    Collection
    ... plasmid can be digested using SapI, which will reveal sticky ends to enable the rapid ligation of annealed...FA, Hsu PD, Lin CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang...CRISPR-Cas9 complex. Konermann S*, Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...
  8. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... derived from P1 E. coli bacteriophage and S. cerevisiae , respectively. Other tyrosine recombinases include... the region of DNA between the target sites is reversed. B) If the target sites are on the same DNA strand...cassette) placed upstream of a gene of interest will prevent gene expression in the absence of Cre. In the presence...Tracing CRISPR Plasmids and Resources Content last reviewed on 31 July 2025. Do you have suggestions for other...
  9. Antibody Guide

    Type
    Collection
    ...Description Configuration and Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears, saliva, mucus... in the cell, and sample processing can hide or reveal epitopes on an antigen. Monoclonal antibodies only...block the membrane to remove excess antibody and prevent unwanted binding. Incubate the membrane with a ...should be run with: a positive control, a sample previously verified to contain your protein of interest ...interest, as described in the IP section above. Reverse the crosslink, isolate DNA, and perform downstream...
  10. Microbiology Resources

    Type
    Collection
    ... S. cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae lab...color. External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (EUROSCARF) (...
  11. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... Lgi1 Mouse Mouse IgG2a 177528 Anti-Brevican [N294A/10R] Brevican Rat Mouse IgG2a 177529 Anti-GluN1 [N308...ARHGAP4 Mouse Mouse IgG2a 190309 Anti-Brevican [N294A/6] Brevican Rat Mouse IgG2a 190311 Anti-NSD1 [N312...Synaptotagmin-7 Mouse Mouse IgG2a 220429 Brevican [N294A/10R-2b] Brevican Rat Mouse IgG2a 220430 BAF53b [N332B...external) Human Mouse 206772 Brevican scFv [N294A/6] N294A/6 scFv Brevican Rat Mouse 206773 GluN3A/NR3A...
  12. Optogenetics Guide

    Type
    Guide
    ...Optogenetics for light control of biological systems. Nat Rev Methods Primers, 2 , 55. https://doi.org/10.1038/...biophysics of microbial rhodopsins in neuroscience. Q Rev Biophys, 57 , e1. https://doi.org/10.1017/S0033583523000033...-activated reversible inhibition by assembled trap; inhibits protein function by reversibly sequestering...Written and reviewed by the Scientific Curation team at Addgene. Last Updated Content last reviewed on 23 Feburary...continue to engineer new tools and improve upon previous tools through engineering, design, and mutagenesis...protein that forms photolabile homodimers with slow reversal kinetics and a UV-B absorption profile 282 BLUF...large multi-subunit complexes in the dark and reversibly dissociate upon blue light stimulation 450 Cryptochromes...
  13. Bacterial Expression Systems

    Type
    Collection
    ...Written and reviewed by the Scientific Curation team at Addgene. Last Updated Content last reviewed on 17 June...out genes or introducing specific mutations can reveal important insights into gene function. These genetic...
  14. Plan Your Experiment

    Type
    Collection
    ...RNA (pegRNA) Prime editor (Cas9 nickase fused to reverse transcriptase (RT)) Can edit within a wider window...the gRNA vector you have chosen, so it is best to review the protocol associated with the specific plasmid...doi.org/10.1038/nbt.3081 PMID: 25357182 Content last reviewed on 11 July 2025....
  15. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...pBAD-PSmOrange2 - Bacterial Expression Return to top Reversibly Photoswitchable (e.g. Off to On to Off) Protein...Lambert Credits Contributing Authors Written and reviewed by Erik Snapp (Albert Einstein College of Medicine...Curation team at Addgene. Last Updated Content last reviewed on 25 September 2025. Do you have suggestions ...
  16. CRISPR Plasmids - Parasites

    Type
    Collection
    ...with CRISPR-Cas9 gene editing have propelled both reverse and forward genetics of these parasites. Moreover...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other...
  17. Mammalian RNAi Tools

    Type
    Collection
    ...Similarities and differences . Advanced Drug Delivery Reviews, 61 (9), 746–759. PubMed (Link opens in a new window..., Zhang, J., & Feng, L. (2023). A comprehensive review of small interfering RNAs (siRNAs): mechanism, ...
  18. CRISPR Plasmids and Resources

    Type
    Collection
    ...modified from a bacterial immune system to a revolutionary tool for genome engineering. CRISPR Topic Page...breaks. Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information into...
  19. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...critical for developing therapeutic strategies to prevent viral spread. As a Biosafety Level 3 (BSL-3) agent... Plasmid Collection Return to top Content last reviewed on 01 October 2025. Do you have suggestions for...
Showing: 61 - 80 of 754 results