We narrowed to 93 results for: SAB;

1. Plasmids 101: Optimizing Plasmid Yields

   Type

   Blog Post

   ...the particular features of your plasmid, it is advisable to check out the genotype of your E. coli to ensure...

2. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   ...for the first time, you've got a collection of reusable, off-the-shelf genetic elements that allow you...

3. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   Blog Post

   ...DNA. Zeocin is an all-in-one antibiotic — it is usable in eukaryotic cell culture, yeast, bacteria, and...

4. CRISPR 101: Making a Knock-In Cell Line

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   Blog Post

   ...can try one or more of the below suggestions. Disable the other repair mechanisms Mammalian cells have...

5. Transferable Skills Guide: Identifying Your Transferable Skills

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   Blog Post

   ...through a nerve wracking commute complete with a disabled train, and now you’re sitting in the lobby of ...

6. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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   Blog Post

   ...synthesis Before you start your cloning, it is advisable to remove unwanted type IIS restriction sites ...

7. Designing Your Chalk Talk for the Academic Job Interview

   Type

   Blog Post

   ...of your chalk talk Bring your own fresh set of erasable markers. You’ll have the colors you want and you...

8. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

   Type

   Blog Post

   ...various research applications, they have become indispensable tools for manipulating cells and organisms. ...

9. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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   Blog Post

   ...23371551. PubMed Central PMCID: PMC3916822. Schopp, Isabel Myriam, et al. "Split-BioID a conditional proteomics...

10. Technique: Probe Phage Genomes for Host Binding Proteins

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    Blog Post

    ...PubMed PMID: 26275959. 2. Weigele, Peter, and Elisabeth A. Raleigh. "Biosynthesis and Function of Modified...

11. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    ...patterns of spread from the injection site it is advisable to try a few different injection titers and volumes...

12. Finding Your Science Policy Path

    Type

    Blog Post

    ...STPF cohort members came to DC to do either a sabbatical year or transition away from academia as tenured...

13. Important Considerations When Using AAVs

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    Blog Post

    .... However, with scAAV the effective genome size usable is shortened (from ~4.8 kB [8] to ~2.3 kB [9])....

14. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post

    ...10.1016/j.chembiol.2019.01.003 Gallagher, S. S., Sable, J. E., Sheetz, M. P., & Cornish, V. W. (2009). ...

15. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post

    ...For many MoClo systems, the individual parts are reusable, saving time and money on future assemblies. If...

16. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    ... (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini (6), and Chari/Mali/Church (7). These reference...

17. Delivery Methods for Generating iPSCs

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    Blog Post

    ...not considered safe for clinical applications. Excisable Cre-lox lentiviral vectors: This approach is ...

18. Zinc Finger Consortium: OPEN Reagents

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    Collection

    ...Strain CSH100 Content blocked, you may need to disable your ad-blocker....

19. CRISPR 101: Cytosine and Adenine Base Editors

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    Blog Post

    ...position upstream of the PAM Kim et al., 2017 SaBE3, BE3 PAM variants, BE3 editing window variants   ...

20. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

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    Blog Post

    ... lab's system called INTEGRATE (Insertion of Transposable Elements by Guide RNA-Assisted Targeting) and...