Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 578 results for: SUB

Showing: 61 - 80 of 578 results

  1. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Posts Eukaryotic parasites continue to cause substantial death and disease in the world. Parasites such...

  3. Chemogenetics Plasmids

    Type
    Collection
    ...actuator. When the luciferase enzyme oxidizes its substrate (luciferin), it generates light that activates...

  4. TALEN Engineering

    Type
    Collection
    ...REAL-Fast assembly method (Reyon et al., manuscript submitted), and the FLASH (Fast Ligation-based Automatable...

  5. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...editing window near the PAM site. Uridine is subsequently converted to thymidine through base excision...

  6. Adenovirus Plasmids

    Type
    Collection
    ...transient transgene expression, as well as the substantial immune response induced by the viral capsid. ...

  7. Retrovirus Plasmids

    Type
    Collection
    ...discuss retrovirus, they typically are referring to a subset of retroviruses called γ-retroviruses. γ-Retroviruses...

  8. Antibody Plasmid Collection

    Type
    Collection
    ...to an antigen, a particular part of a foreign substance. In addition to being useful for our bodies to...

  9. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...sequence remains unchanged and available as a substrate for another round. Figure 1: Overview of prime...

  10. Microbiology Resources

    Type
    Collection
    ...Heterologous protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth Lab pOSIP plasmid...

  12. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...enzyme digest (subcloning), including design and experimental procedures.... Protocols Subcloning Plasmid Cloning...Cloning by Restriction Enzyme Digest (aka Subcloning) You may also like... Restriction Digest of Plasmid...out our Empty Backbone Reference . Background Subcloning by restriction digest is a commonly used lab ...YGOI is in a bacterial expression vector. Using subcloning, you can easily move YGOI into a mammalian expression...find two different restriction enzymes for your subcloning. It is also possible to use a single enzyme, ...

  13. TALEN Plasmids and Kits

    Type
    Collection
    ...expression of fluorescent TAL effectors to visualize subcellular positioning of target DNA sequences in living...

  14. Lentivirus Plasmids

    Type
    Collection
    ...Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses, are some of the most common and...

  16. CRISPR References and Information

    Type
    Collection
    ...activation). Genome support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( C. elegans ...

  17. DNA Quantification

    Type
    Protocol
    ... of a substance in that liquid. In order to accurately measure the concentration of a substance based ...to determine the concentration of a particular substance in that liquid. Molecules absorb different wavelengths...need to know the wavelength of light that your substance maximally absorbs. In the case of nucleic acid...

  19. Western Blot

    Type
    Protocol
    ...powder 96-well microtiter plate Chemiluminescence substrate Plastic wrap Primary antibody Secondary antibody...absorbance of the duplicate samples on the plate. Subtract the average absorbance of the blank from all of...shaking platform. Prepare the chemiluminescence substrate by mixing 1:1 reagent A to reagent B. Gently incubate...

  20. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...,5'-tetramethyl-[1,1'-biphenyl]-4,4'-diamine) substrate kit, Pierce 34021 Tween-20, VWR 0777-1L Foil Before...Solution and 5 mL Peroxide Solution from the TMB substrate kit. Cap the tube and invert several times to ...secondary antibody for 2 hours, reacted with TMB substrate and the reaction stopped after 15 minutes. Absorbance...
Showing: 61 - 80 of 578 results