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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 579 results for: SUB

Showing: 61 - 80 of 579 results

  1. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...sequence remains unchanged and available as a substrate for another round. Figure 1: Overview of prime...

  2. Microbiology Resources

    Type
    Collection
    ...Heterologous protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth Lab pOSIP plasmid...

  4. TALEN Plasmids and Kits

    Type
    Collection
    ...expression of fluorescent TAL effectors to visualize subcellular positioning of target DNA sequences in living...

  5. Lentivirus Plasmids

    Type
    Collection
    ...Plasmids Envelope Plasmids Resources Lentiviruses, a subset of retroviruses, are some of the most common and...

  6. CRISPR References and Information

    Type
    Collection
    ...activation). Genome support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( C. elegans ...

  7. Bacterial Expression Systems

    Type
    Collection
    ...periplasmic protein expression in E. coli , Bacillus subtilis , and Pichia pastoris . The EcoFlex MoClo Toolkit...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ...experimental systems. 2nd and 3rd Generation Systems Subsequent efforts have improved the components of these...

  9. Plan Your Experiment

    Type
    Collection
    ...variety of modifications and will likely contain a subset of wild type cells. If you are screening a variety...

  10. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...enzyme digest (subcloning), including design and experimental procedures.... Protocols Subcloning Plasmid Cloning...Cloning by Restriction Enzyme Digest (aka Subcloning) You may also like... Restriction Digest of Plasmid...out our Empty Backbone Reference . Background Subcloning by restriction digest is a commonly used lab ...YGOI is in a bacterial expression vector. Using subcloning, you can easily move YGOI into a mammalian expression...find two different restriction enzymes for your subcloning. It is also possible to use a single enzyme, ...

  12. DNA Quantification

    Type
    Protocol
    ... of a substance in that liquid. In order to accurately measure the concentration of a substance based ...to determine the concentration of a particular substance in that liquid. Molecules absorb different wavelengths...need to know the wavelength of light that your substance maximally absorbs. In the case of nucleic acid...

  13. Western Blot

    Type
    Protocol
    ...powder 96-well microtiter plate Chemiluminescence substrate Plastic wrap Primary antibody Secondary antibody...absorbance of the duplicate samples on the plate. Subtract the average absorbance of the blank from all of...shaking platform. Prepare the chemiluminescence substrate by mixing 1:1 reagent A to reagent B. Gently incubate...

  14. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...,5'-tetramethyl-[1,1'-biphenyl]-4,4'-diamine) substrate kit, Pierce 34021 Tween-20, VWR 0777-1L Foil Before...Solution and 5 mL Peroxide Solution from the TMB substrate kit. Cap the tube and invert several times to ...secondary antibody for 2 hours, reacted with TMB substrate and the reaction stopped after 15 minutes. Absorbance...

  15. General Transfection

    Type
    Protocol
    ...function. We specifically use this protocol with a subclone of HEK293T cells optimized for viral production...Workflow Timeline Day 0: Seed HEK293T cells (or a subclone of HEK293T optimized for viral production) Day...

  16. Pipetting Protocol

    Type
    Protocol
    ... lower the pipette tip into the liquid. Do not submerge the pipette itself into the liquid and avoid touching...contaminating the contents. Once the end of the tip is submerged, slowly release the plunger, allowing it to go...

  17. Pouring LB Agar Plates

    Type
    Protocol
    ... a water bath at 60 ℃ with sufficient water to submerge ~75% of the bottle containing your molten gel ...removing anything from the autoclave. Partially submerge your molten gel-mix in the 60 ℃ water bath. Pro-Tip...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...genes, and is unique in that it is composed of two sub-pools. The first is a base “90k library” that contains...using CRISPR-X and subsequent FACS sorting. They also mutagenized proteasome subunit PSMB5 to find mechanisms...Every quarter we highlight a subset of the new plasmids in the repository through our hot plasmids articles...mRNA binding, and these sequence changes are subsequently identified through transcriptome sequencing.... Guler Lab engineered an actuator in which two subunits of the paramagnetic ferritin protein were tethered...channel). The Guler lab optimized the actuator’s subcellular localization using trafficking signals and named...

  20. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...can be converted into lentiviral particles for subsequent infection of a target cell line. Once introduced...toxic to some cell lines. In these cell lines, substitute protamine sulfate for polybrene. c. Add lentiviral...
Showing: 61 - 80 of 579 results