We narrowed to 765 results for: Son

...James M. Wilson 105558 pENN.AAV.CamKII 0.4.Cre.SV40 CamKII none 1, 5, 9, rg* James M. Wilson 105537 pENN.AAV.CMVs.Pl.Cre.rBG...James M. Wilson 105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 James M. Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre...James M. Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn none 1, 2, 5, 8, 9, rg* James M. Wilson 105555 pENN.AAV.hSyn.Cre.hGH...pAAV.GFAP.Cre.WPRE.hGH GFAP none 5, PHPeB James M. Wilson 196410 AAV-GfaABC1D-Cre-4x6T GfaABC1D none 5 Stanley...107788 AAV.rTH.PI.Cre.SV40 rTH none 9, rg* James M. Wilson 24593 AAV-pgk-Cre PGK none rg* Patrick Aebischer... pENN.AAV.hSyn.Cre.hGH Syn none 9 James M. Wilson 107312 AAV-hSyn-mCherry-P2A-Cre-WPRE Syn mCherry 1 Hui... 107787 AAV.TBG.PI.Cre.rBGe TBG none 8 James M. Wilson Dre AAV ID Name Promoter Fluorophore Serotype(s...

...acceptor) FRET Robert Campbell , Michael Davidson Michael Davidson , Nathan Shaner , Roger Tsien 54575 54771...Timers Return to top Protein Interactions Förster resonance energy transfer (FRET) and bimolecular fluorescence...acceptor) FRET/Dual FRET Robert Campbell Michael Davidson , Nathan Shaner , Roger Tsien 54553 54723 mTFP1...acceptor) FRET/Dual FRET Robert Campbell , Michael Davidson 54571 54856 mT-Sapphire-pBAD tdTomato-pBAD mT-... Clover (donor) mRuby2 (acceptor) FRET Michael Davidson 18856 pGWF1 ECFP (donor) Venus (acceptor) FRET...IPTG Escherichia coli , Acinetobacter baumannii Jason Peters 127088 pMS17 tcp830 Anhydrotetracycline (... (firefly luciferase) Mycobacterium sp. Brian Robertson , Siouxsie Wiles 26161 pMV306hsp+LuxG13 Promoter...

...105448-AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore...pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the ...pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE Expresses bicistronically soma-targeted ChrimsonR in frame...Chemogenetics Cre-dependent expression plasmid Scott Sternson 119741-AAV9 AAV SYN flex PSAM4 GlyR IRES EGFP ...Chemogenetics Cre-dependent expression plasmid Scott Sternson 119742-AAV5 AAV SYN PSAM4 GlyR IRES EGFP Chemogenetics...Chemogenetics expression plasmid Scott Sternson 119744-AAV5 AAV CAMKII PSAM4 GlyR IRES EGFP Chemogenetics...

...Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR. 2016. Naturally occurring off-switches...MF, Hidalgo-Reyes Y, Wiedenheft B, Maxwell KL, Davidson AR. 2015. Multiple mechanisms for CRISPR-Cas inhibition...26416740 Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR. 2013. Bacteriophage genes that inactivate ...132-6. PMID: 23942116 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes...Cell . 159(3):647-6. PMID: 25307932 Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-...Topkar VV, Nguyen NT, Zheng Z, Gonzales AP, Li Z, Peterson RT, Yeh JR, Aryee MJ, Joung JK. 2015. Engineered...Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. 2015. Multicolor CRISPR labeling of chromosomal...

...nerve cell function within the cell. Parkinson's Disease Parkinson’s disease (PD) is a chronic and progressive...neurodegenerative diseases include Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease. These diseases...as early-onset, where symptoms appear between a person’s thirties and mid-sixties, or late-onset, where...where symptoms appear during or after a person's mid-sixties. The early-onset form accounts for less than...created with support from the BRAIN Initiative. Jackson Laboratory (Link opens in a new window) A collection...A foundation dedicated to finding a cure for Parkinson's disease through an aggressively funded research... of improved therapies for those living with Parkinson's today. The Michael J. Fox Foundation has made...

...website. For a comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag, and...1998) References George N, Pick H, Vogel H, Johnsson N, Johnsson K. 2004. Specific labeling of cell surface...9712910 Vivero-Pol L, George N, Krumm H, Johnsson K, Johnsson N. 2005. Multicolor imaging of cell surface...

...James M. Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn Cre expression Recombinases James M. Wilson 105558...pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control James M. Wilson 116869 pAAV-CAG-H2B-GFP CAG GFP Control Loren Looger...EGFP-tagged Cre expression Recombinases James M. Wilson 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII...Cre.SV40 CamKII Cre expression Recombinases James M. Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression...AAV.rTH.PI.Cre.SV40 rTH Cre expression Recombinases James M. Wilson 55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a Flpo and...Activator Optogenetics Edward Boyden 59171 pAAV-Syn-ChrimsonR-tdT Syn Activator Optogenetics Edward Boyden 83898...

...Structure Plasmids mCrimson3 588 615 Monomer mCrimson3-N1 - Mammalian Expression mCrimson3-C1 - Mammalian ...Mammalian Expression E2-Crimson 611 646 29 4.5 24 min Tetramer pAAV-CAG-E2-Crimson - Mammalian AAV Expression...Expression pEB2-E2-Crimson - Bacterial Expression TagRFP657 611 657 0.34 5.3 27 min Prone to dimerization...Resources Addgene Collections Empty Backbones Michael Davidson Fluorescent Protein Collection Luciferase Plasmid...

...James M. Wilson 112864 pAAV2/8 AAV8 AAV packaging plasmid, expressing Rep2 and Cap8 James M. Wilson 112865...packaging plasmid, expressing Rep2 and Cap1 James M. Wilson 232199 pAAV2/2 AAV2 AAV packaging plasmid, expressing...packaging plasmid, expressing Rep2 and Cap9 James M. Wilson 240486 pAAV2/11 v1 AAV11 AAV packaging plasmid,...plasmid, expressing Rep2, and rh10 capsid James M. Wilson 81070 rAAV2-retro helper AAV retrograde AAV packaging...

...using sonication to break DNA up into fragments of 300-1000 bps in length. Note: This sonication process...may need additional processing steps, such as sonication. Denature proteins, using heat and/or chemicals...housekeeping genes). This allows for relative comparison of expression between different samples, by normalizing...normalizing protein expression to the controls before comparison. Alternatively, the samples can be normalized...ChIP protocols use enzyme digestion instead of sonication. This approach is gentler but results in non-...isolated and analyzed. Validation relies on size comparison of peptides. Independent antibody validation ...

...Link opens in a new window) PMID: 17289941 2010 - Allyson Muotri's lab develops the first iPSC model for ...window) PMID: 26944080 (Link opens in a new window) Alysson Muotri Q83X C247T Q83X M Fibroblasts & iPSC (Link...window) PMID: 26944080 (Link opens in a new window) Alysson Muotri c.806delG 806delG G269Afs*20 M Fibroblasts...patient-derived iPSCs (Link opens in a new window) Jackson Labs - mouse line developer and repository, and...Pediatr Neurol . 52, 585-591.e2. PMID: 25801175 Tillotson and Bird. 2019. The Molecular Basis of MeCP2 Function...

... Collections MJFF Parkinson's Plasmid Resource Michael J. Fox Foundation Parkinson's Disease Plasmid Resource...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation... researchers to assemble and share tools for Parkinson's disease research. This collection is part of ...

...patient-specific or disease-specific cells. For this reason, iPSCs have become an excellent unlimited stem ...Cells. Science. 2007 Dec 21;318(5858):1917-20. Thomson Lentivirus Human Doxycycline-inducible expression...piggyBac Mouse Doxycycline-inducible piggyBac transposon reprogramming system; expression of mouse Oct4... pii: cells9112451. doi: 10.3390/cells9112451. Ottosson Stem Cell Research Plasmids This table contains...

...synthetase M. maripaludis phosphoserine Bacterial Jason W Chin 174078 pDule-3-nitroTyrosine (A7) 3NY (A7...MmPylRS_MmtRNA-Pyl-opt(UGA) PylRS M. mazei Bacterial TGA Jason W Chin 174516 pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe... AfTyrRS CbzK and p-I-Phe Bacterial TCG and TAG Jason W Chin 174718 pRSF-G1mCNPRS G1mCNPRS Methanogenic...coumarin-yl) ethylglycine Bacterial TAG Kenneth Johnson 198323 pRSF-G1TMSNKRS M. archaeon TMSNK Bacterial..., TCA, or TAG codons in all open reading frames Jason W Chin 174514 Syn61Δ3(ev5) No TCG, TCA, or TAG codons... codons. Deletion of serT, serU, and prfA genes Jason W Chin 182773 C321.∆A ompT- rne- lon- Recoded E....

... Inhibition IRES EGFP Cre-dependent 5, 9 Scott Sternson 119742 AAV SYN PSAM4 GlyR IRES EGFP PSAM4 GlyR...GlyR - Inhibition IRES EGFP none 5 Scott Sternson 119744 AAV CAMKII PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition...Inhibition IRES EGFP none 5 Scott Sternson 121538 pAAV SYN1 HA-hM4D(Gi) hM4D(Gi) - Inhibition HA fusion...hM4D(Gi) - Inhibition GFP Cre-dependent 1 Scott Sternson 228902 pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine HCAD...

...Additional Resources Förster (or Fluorescence) Resonance Energy Transfer (FRET) is a process by which energy...multiphoton spectral imaging and its use for measuring resonance energy transfer mCerulean, mVenus, and Amber (... positive controls for many FRET pairs Michael Davidson Lab (Unpublished) FRET Indicators - Live and Fixed...fluorescent protein acceptor for Bioluminescence Resonance Energy Transfer (BRET) . Browse Addgene's Luciferase...

...Berndt A, Grosenick L, Zalocusky KA, Bernstein H, Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve ...opens in a new window) Wick ZC, Tetzlaff MR, Krook-Magnuson E. 2019. A novel population of long-range inhibitory...Tzortzi O, Weglage M, Märtin A, Xuan Y, Parent M, Johansson Y, Fuzik J, Fürth D, Fenno LE, Ramakrishnan C,...Hyun M, Umadevi Venkataraju K, Straub C, Wang W, Robertson K, Osten P, Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal...

...permuted fluorescent protein (cpFP) or fluorescence resonance energy transfer (FRET) efficiency of a pair of...locations. PLoS One. 2012;7(11):e49371. Direct comparison of a genetically encoded sensor and small molecule... levels and metabolism (FLIPmal) Fluorescence Resonance Energy Transfer sensors for quantitative monitoring... Science. 2018 Sep 14;361(6407):1122-1126. Kai Johnsson NADPH/NADP+ or NAD+ Snifit FRET-based sensors ...dinucleotides. eLife. 2018 May 29;7. pii: 32638. Kai Johnsson NADPH/NADP+ NAPstar biosensors for NADPH/NADP+...Am Chem Soc. 2012 Nov 21;134(46):19026-34. Kai Johnsson Glutamate Fourth generation glutamate sensors ...

...intron-lacZ/NLS References Portales-Casamar, E., Swanson, D. J., Liu, L., de Leeuw, C. N., Banks, K. G.,... Palma, B., Palmquist, D. L., Schmouth, J. F., Swanson, M. I., Tam, B., Ticoll, A., Turner, J. L., Varhol...Varhol, R., Vermeulen, J., Watkins, R. F., Wilson, G., Wong, B. K., Wong, S. H., Wong, T. Y., Yang, G. ... Holt, R. A., Goldowitz, D., Wasserman, W. W., Simpson, E. M. (2010). A regulatory toolbox of MiniPromoters...

... CMV EGFP Control Wilson 105547 pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control Wilson 114469 pAAV-CaMKIIa-mCherry...Cre-EGFP expression Cre Wilson 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP Cre-expression Cre Wilson 107738 pAAV-hSyn-...