We narrowed to 888 results for: TRI

...Dyche Mullins 41147 pEGFP Centrin2 Centrioles (dependent on cell cycle) Centrin-2 EGFP Erich Nigg 41151 ...41151 pEGFP Cep170 C-term Centrioles (dependent on cell cycle) Cep170 EGFP Erich Nigg 41163 pEGFP PICH Centromeres...EB1 EGFP Ken-Ichi Takemaru 122871 GFP-hCCDC11 Centriolar satellites CCDC11 EGFP Ken-Ichi Takemaru 54234...-alpha Tubulin Microtubules alpha-Tubulin EGFP Patricia Wadsworth 27123 eTC GFP beta-actin full length...

...most widely known of these products is TRIzol® (also called TRI Reagent®, RNAzol®, QIAzol®  and sold by...homogenate and shaken. After a quick centrifugation (not ultracentrifugation!), the layers of phenol and chloroform...After homogenization in TRIzol®, insoluble material is removed via centrifugation and the supernatant is... loaded onto cesium-chloride gradients and ultracentrifuged for up to 20 hours to separate the intact ...have, access to one or more large, expensive ultracentrifuge.  Figure 1: An outline of the different...out to develop a protocol that skipped the ultracentrifugation altogether. Chomczynski and Sacchi showed... by many different companies). TRIzol® is an all-in-one acid-guanidinium-phenol solution that combines...

...101781 CETN2-mTagRFP-T AICSDP-22 mTagRFP-T Centrin-2 Centrioles 101782 LAMP1-mEGFP AICSDP-19 mEGFP LAMP-...lines have been deposited here with Addgene for distribution to the research community. With these plasmids...Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin Matrix Adhesions 87421 TUBA1B-mEGFP AICSDP-4 mEGFP Alpha-tubulin...

...amplification Viral vector production Density centrifugation purification Addgene’s comprehensive quality... and results through Addgene’s Data Hub . By contributing your AAV vector experience, from methods to ...titration, and quality control measures. We have distributed preps around the globe and shared our well-developed...lab, and even managing legal permissions and distribution. Troubleshooting If your answer isn’t in the...

...candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2 SESN3 Sestrins 1, 2, or 3 Siah Siah E3 ubiquitin...stresses like DNA damage, oxidative stress, and nutrient deprivation. p53 is activated through both phosphorylation...ribonucleotide reductase M2 B (RRM2B) PAG608 Zinc finger, matrin-type 3 PAI Serpin peptidase inhibitor, clade E ...

...Enzyme Collection DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...polymerases, DNA ligases, reverse transcriptases, and restriction enzymes. These enzymes are useful in both basic...the links below: DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...-phi29pol phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert ...

...N1 , mVenus N1 mTFP1 mCitrine 462 0.85 529 94,000 0.74 5.9 2.6 mTFP1-N1 , mCitrine-pBAD EGFP mCherry 488...pNCS-CyOFP , mCardinal-N1 mAmetrine* tdTomato 406 0.58 581 138,000 0.69 6.5 7.1 mAmetrine-N1 , tdTomato-N1 mTurquoise2...

... this effort by providing a platform for the distribution of plasmid-based tools that are useful for COVID... a for-profit entity, please see our Plasmid Distribution to Industry page. ID Plasmid Description Industry...Collection – Addgene partnered with Ginkgo Bioworks to distribute SARS-CoV-2 plasmids for E. coli , yeast, and ... plasmids - a cysteine endosomal protease that triggers proteolysis of SARS-CoV Spike protein, activating...

...confused, bored, or particularly excited about? If you tried tweaking your pitch throughout the conference, what...

...answer should come soon, but not instantly. Addgene tries to get back to customers within 24 hours, and we...time at Addgene, I’m confident that these tips and tricks will apply to any biology-related customer support...

...writing three years ago on a field trip in Indonesia. Since then, I have tried many things: photography, making...This post was contributed by guest blogger Sarah Schmidt, a Marie Curie Fellow at The Sainsbury Laboratory...hosted by Nature), and Addgene are open to guest contributions. If you decide to start your own blog, check...

...’t tell if the bags contained flora or fauna. I tried to find out what certain ingredients might be used...very welcoming team from China’s Ribobio, the illustrious speaker list attracted a host of sponsors based...and depositors. China is in one of the top 10 countries in number of plasmid requests from Addgene. ...

...reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming...conversion of mouse fibroblasts to self-renewing, tripotent neural precursor cells. Proc Natl Acad Sci U S...Spiny Neurons Lentiviral Human Generation of Human Striatal Neurons by MicroRNA-Dependent Direct Conversion.... 2017 Aug;9(8):1117-1131. Parmar Fibroblasts Dentritic Cells Lentiviral Mouse Direct reprogramming of...

...activity with endogenous cell-type specific miRNAs to restrict CRISPR editing only to the target tissue. Browse... Acidaminococcus sp. ). The popularity can be attributed to Cas12a’s many benefits. Cas12a exhibits increased... quantification and can be used on lateral flow strips. Other Cas enzymes can be added to SHERLOCKv2 to...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations...924–927. PMID: 30377362 Casini, A., Olivieri, M., Petris, G., Montagna, C., Reginato, G., Maule, G., Lorenzin... (12), 770–788. PMID: 30323312 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S....152 (5), 1173–1183. PMID: 23452860 Savic, D., Partridge, E. C., Newberry, K. M., Smith, S. B., Meadows...

... viral vector preps are purified by density centrifugation. Addgene’s comprehensive high-quality quality...genome sequencing (VGS). In-stock AAV preps are distributed as 100 µL solutions of purified viral particles...Samples Small samples of control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 6, 8, 9, 11, and...

...and Patrick Salmon 35616 pCAG-VSVG VSV-G-expressing envelope plasmid. Arthur Nienhuis and Patrick Salmon...35614 pBS-CMV-gagpol Contains MoMLV gag and pol . Patrick Salmon Return to top Popular Gamma-Retroviral Envelope...

...Tommaso Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR none Constitutive 9 Tommaso Patriarchi Oxytocin...pAAV-Ef1a-fDIO-GCaMP6f EF1a GCaMP6f none Flp dependent 1 Patricia Jensen 135632 pAAV-S5E2-GCaMP6f E2 GCaMP6f none...pAAV-Ef1a-fDIO-JRGECO1a EF1a jRGECO1a none Flp dependent 1 Patricia Jensen Calcium Sensor: sRGECO 137125 pAAV-Ef1a-sRGECO...

...microbiology resources. The plasmids Addgene distributes cannot be used to reconstitute self-replicating...Addgene continues to monitor its library and distributions in accordance with export laws and biosafety...Bordetella pertussis Campylobacter sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus sp. ...

...caused by abnormal expansion of a repeating CAG triplet series within the huntingtin (HTT) gene. An individual... the disease, a small portion of cases can be attributed to known genetic factors such as defects in LRRK2...organelles. (Mattia et al. EMBO Rep. 2018) Study intrinsically disordered protein regions with FUS and HNRNPA1...

...answers come sooner rather than later. Once you’ve tried it, let us know how xCas9 is working for you in ...another method to create point mutations, also has a strict editing window about 15 nt upstream of the PAM....included continuous outflow of phage, increasing stringency and promoting survival of only the broadest-targeting...