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  1. Lab to Office Culture Shock

    Type
    Blog Post
    ...     Over the last decade, my peers and I have tried to move away from calling non-academic careers and...hands-on lab opportunities. You can get your hands in nitrile gloves again! The opportunities are there once ...

  2. Immunology Research Plasmids and Resources

    Type
    Collection
    ...KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9 TNFRSF10C tumor necrosis factor... to a specific trigger. Symbol Name Synonyms ACVR1B activin A receptor, type IB ACTRIB, ACVRLK4, ALK4,... A receptor, type IIA ACTRII, ACVR2 ACVR2B activin A receptor, type IIB ACTRIIB, ActR-IIB, MGC116908 ACVRL1...secretagogue receptor - GIP gastric inhibitory polypeptide - GIPR gastric inhibitory polypeptide receptor...FMRFAL NPPA natriuretic peptide precursor A ANF, ANP, ATFB6, CDD-ANF, PND NPPB natriuretic peptide precursor...

  3. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...CMV ALS Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS Trina Schroer 51412 CMV-p150 DCTN1 CMV ALS Trina Schroer...H1 ALS Patrick Aebischer 10881 pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch SOD1 H1 ALS Patrick Aebischer...CMV Parkinson's Patrick Aebischer 36070 pAAV asyn S87A/S129A SNCA CMV Parkinson's Patrick Aebischer 36071...EGFP OPTN GFP CMV ALS Beatrice Yue 68839 pOPTN1-209-EGFP OPTN GFP CMV ALS Beatrice Yue 68840 pOPTN1-424...EGFP OPTN GFP CMV ALS Beatrice Yue 68841 pOPTN217-577-EGFP OPTN GFP CMV ALS Beatrice Yue 68842 pOPTN217-...EGFP OPTN GFP CMV ALS Beatrice Yue 68843 pOPTN425-577-EGFP OPTN GFP CMV ALS Beatrice Yue 68846 pR96L-EGFP...pR96L-EGFP OPTN GFP CMV ALS Beatrice Yue 68847 pD474N-EGFP OPTN GFP CMV ALS Beatrice Yue 68848 pE478G-EGFP OPTN...

  4. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-N1 - Mammalian...Expression mAmetrine 406 526 26 6 0.8 hr Monomer (A206K) pBad-mAmetrine - Bacterial Expression mAmetrine-N1 -...- Mammalian Expression mCitrine 516 529 59 5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C)... Expression pCS2+8CmCitrine - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NmCitrine - Zebrafish/Xenopus...Mammalian Expression mCitrine-C1 - Mammalian Expression mCitrine-pBAD - Bacterial Expression YPet 517 530 80 ... - Mammalian Expression mAmetrine-C1 - Mammalian Expression LSSmOrange 437 572 23 5.7 2.3 hr Monomer pLSSmOrange-C1...Mammalian Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes many...

  5. Chemogenetics AAV Preps

    Type
    Collection
    ...pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor (KOR) - Inhibition IRES mCitrine Cre-dependent 8 Roth...-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine Cre-dependent 8 Roth ...-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine Cre-dependent 8 Roth ...pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine none 2, 8 Roth 50476 ...pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine rM3D(Gs) - Activation HA fusion and IRES mCitrine none 5 Roth 50478 pAAV-GFAP-hM3D...element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity Cre-dependent Flp-dependent...

  6. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...-CDS issues. Prohibited Restriction Sites: EcoRI, XbaI, SpeI, PstI Restriction Sites to Avoid: NotI For...Coli genome. Prohibited Restriction Sites: EcoRI, SpeI, NheI, PstI Restriction Sites to Avoid: PvuII, ... use of assembly standards – combinations of restriction sites which preserve a standard prefix and suffix...assembly standards most commonly used by part registries, including the specific prefix and suffix sequences... reverse complimented for primer design. The restriction enzymes used for assembly are the prohibited ... Employs high-efficiency enzymes. Prohibited Restriction Sites: EcoRI, BglII, BamHI, XhoI For more info...giving in-frame scar encoding Thr-Ala. Prohibited Restriction Sites: EcoRI, XbaI, SpeI, PstI For more info,...

  7. Rett Syndrome

    Type
    Collection
    ...opens in a new window) ClinicalTrials.gov - a list of Rett syndrome clinical trials (Link opens in a new...in a new window) PMID: 17289941 2010 - Allyson Muotri's lab develops the first iPSC model for Rett syndrome...: 26944080 (Link opens in a new window) Alysson Muotri Q83X C247T Q83X M Fibroblasts & iPSC (Link opens...: 26944080 (Link opens in a new window) Alysson Muotri c.806delG 806delG G269Afs*20 M Fibroblasts & iPSC...new window) HRV Metrics Toolbox - algorithms for data analysis to calculate heart rate variability (Link...in Australia: a review of the epidemiology. J Pediatric . 148(3):347-352. (Link opens in a new window)...Return to top Help us improve this resource by contributing your plasmids! To get started with your deposit...

  8. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Dyche Mullins 41147 pEGFP Centrin2 Centrioles (dependent on cell cycle) Centrin-2 EGFP Erich Nigg 41151 ...KozATG-myc-dL5-2XG4S-mCer3-SKL Peroxisome SKL tripeptide, peroxisome transport signal dL5 FAP; mCerulean3...41151 pEGFP Cep170 C-term Centrioles (dependent on cell cycle) Cep170 EGFP Erich Nigg 41163 pEGFP PICH Centromeres... mCherry Gia Voeltz 89472 GFP-hChibby1 Mother Centrioles / Ciliary Base Chibby1 GFP Ken-Ichi Takemaru ...Takemaru 89770 pLenti-EGFP-hChibby1 Mother Centrioles / Ciliary Base Chibby1 EGFP Ken-Ichi Takemaru 118084 pLenti-EB1...EB1 EGFP Ken-Ichi Takemaru 122871 GFP-hCCDC11 Centriolar satellites CCDC11 EGFP Ken-Ichi Takemaru 54234...-alpha Tubulin Microtubules alpha-tubulin EGFP Patricia Wadsworth 27123 eTC GFP beta-actin full length...

  9. Antibody Production

    Type
    Collection
    ...undergo stringent quality control by Addgene or a trusted quality control partner before distribution. Details...Production Antibody Production Many antibodies distributed by Addgene have been produced in-house by Addgene...contacting [email protected] . Some of the antibodies distributed by Addgene were developed by the Institute for..., antibodies undergo a buffer exchange using centrifugal columns. The final formulation buffer is phosphate-buffered.... Only antibody samples with >90% purity are distributed. Quality Control Each lot of antibody undergoes...

  10. TALEN Guide

    Type
    Collection
    ...fingers that recognize various nucleotide triplets. With some trial and error, ZF arrays are able to recognize...zinc finger (ZF) nuclease. Zinc fingers recognize triplets and the FokI nuclease operates as a dimer, cutting...their limitations, however. Not every nucleotide triplet has a corresponding zinc finger, and interactions... targets are confined to sequences composed of triplets with corresponding zinc fingers, potential targetable...genome are every 500 bp. TAL effectors have some restrictions (for example, the target must start with a T...

  11. Antibody Guide

    Type
    Collection
    ...light orange, that bind to a specific antigen, triggering an immune response. The stem of the Y structure...single antibody signal. Here, HRP is used as a colorimetric, in which the reaction produces a colored product...the amount of enzyme present in each well. In colorimetric assays, protein amount can be determined by ..., lyse, and then collect protein extract by centrifugation or affinity columns. Mix samples with a primary...immobilized on agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic... your samples using permeabilization, antigen retrieval, and/or sectioning. Block to reduce non-specific...tissue sections are assayed and the extracellular matrix remains intact. The signaling molecule may be a...

  13. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...was first described in 1966 by Austrian physician, Andreas Rett, who tried to raise awareness and published...binds to methylated CpG dinucleotides and CAC trinucleotides during neuronal maturation (Tillotson & Bird...in which too much of the MECP2 protein is as detrimental as too little, resulting in MECP2 duplication...advance their respective gene therapies to clinical trials in 2021. Figure 1: Schematic of the human...

  14. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...for most purposes, but what do you do when you’ve tried a common strain and don’t get the desired level ...inactivated native restriction/methylation system. This means the strain can neither restrict nor methylate...expression of T7 RNAP from the genome; does not restrict methylated DNA fhuA2 lacZ::T7 gene1 [lon] ompT...adding the inducer. Growth Media: Changing media is tricky, because there can be a trade-off between growth...about your favorite protein expression tips and tricks! Leave a comment below or browse Addgene’s collection...

  15. Brain Initiative Collection

    Type
    Collection
    ...Addgene distributes ready-to-use viral preps made from plasmids in our collection! AAV are distributed as ...NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection . The NeuroMab Facility and James Trimmer have produced...Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven KOR DREADD...recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use recombinant antibodies made from...that are currently in our repository. AAV are distributed as 100 µL of purified viral particles in solution...

  16. Viral Production

    Type
    Collection
    ...subjected to quality control by Addgene before being distributed to customers. Details about our production protocols...control are described below. AAV Production AAV distributed by Addgene has been produced either in-house ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins VP1, VP2...containing lentivirus is first cleared by low-speed centrifugation and then by filtration through a 0.45 µm polyethersulfone...

  17. Luciferase Plasmid Collection

    Type
    Collection
    ...secreted version of the protein is available – these attributes have made it popular with scientists since its...enchancer regions in Drosopholia Herman Wijnen 126033 pTETRIS-cargo Firefly For use as a reporter of the ability... in plants, co-expressed with mScarlet-I for ratiometric imaging David Nelson 141301 pRATIO1267 RedLuc...plants, co-expressed with Gaussia luciferase for ratiometric imaging David Nelson 212935 pGL4.84(hRlucCP/Puro...Lentiviral expression of firefly luciferase Brent Stringer 102320 FUW-RLuc-T2A-PuroR Renilla Ubc Lentiviral...

  18. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...101781 CETN2-mTagRFP-T AICSDP-22 mTagRFP-T Centrin-2 Centrioles 101782 LAMP1-mEGFP AICSDP-19 mEGFP LAMP-...lines have been deposited here with Addgene for distribution to the research community. With these plasmids...Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin Matrix Adhesions 87421 TUBA1B-mEGFP AICSDP-4 mEGFP Alpha-tubulin...

  19. AAV Packaged on Request

    Type
    Collection
    ...amplification Viral vector production Density centrifugation purification Addgene’s comprehensive quality... and results through Addgene’s Data Hub . By contributing your AAV vector experience, from methods to ...titration, and quality control measures. We have distributed preps around the globe and shared our well-developed...lab, and even managing legal permissions and distribution. Troubleshooting If your answer isn’t in the...

  20. p53 Pathway

    Type
    Collection
    ...candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2 SESN3 Sestrins 1, 2, or 3 Siah Siah E3 ubiquitin...stresses like DNA damage, oxidative stress, and nutrient deprivation. p53 is activated through both phosphorylation...ribonucleotide reductase M2 B (RRM2B) PAG608 Zinc finger, matrin-type 3 PAI Serpin peptidase inhibitor, clade E ...
Showing: 61 - 80 of 884 results