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We narrowed to 506 results for: Trac;

Showing: 61 - 80 of 506 results

  1. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...with an empty tube. Alternatively, you can just subtract the weight of the empty tube from the weight of...the (Link opens in a new window) QIAquick Gel Extraction Kit . Always follow the manufacturer's instructions...

  2. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ... glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract and dH 2 O to 400 mL Note: If your lab has pre-...mL Kanamycin 50 µg/mL Spectinomycin 50 µg/mL Tetracycline 10 µg/mL Tips and FAQ What is the difference...

  3. Centrifugation

    Type
    Protocol
    ...experimental procedures, such as minipreps and RNA extractions. Plus, centrifuges are useful for simply collecting...cousins, but can hold much larger containers. Ultracentrifuges are also generally floor sized centrifuges...

  4. Plasmids for Stem Cell Research

    Type
    Collection
    ...non-integrating episomes to allow sorting and dosage tracking of reprogramming factors Fluorescent tagged episomals...

  5. Pouring LB Agar Plates

    Type
    Protocol
    ... g pre-mixed powder consisting of: 5.0 g yeast extract 10.0 g peptone from casein 10.0 g sodium chloride.../mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg/mL Notes: Unless otherwise indicated...

  6. Western Blot

    Type
    Protocol
    ... a sonication step may be required for hard to extract proteins such as those in the nucleus. Incubate...absorbance of the duplicate samples on the plate. Subtract the average absorbance of the blank from all of...

  7. Lentivirus ddPCR Titration

    Type
    Protocol
    ... min at 100 x g . Gently aspirate supernatant. Extract genomic DNA according to the GeneJet Genomic DNA...used to transduce HEK293T cells. Genomic DNA is extracted from the target cells and assayed for integrated...

  9. Weighing Reagents Protocol

    Type
    Protocol
    ...Pro-Tip When you weigh out a reagent, you may leave trace amounts of it on the balance. Therefore, it’s best...

  10. Video Library

    Type
    Protocol
    ...cells AAV Purification by Iodixanol Gradient Ultracentrifugation Protocol Mulitchannel Pipetting Technique...

  13. CRISPR Library Amplification

    Type
    Protocol
    ...pre-amplified stock. A last option is to perform a gel extraction of either the original sample or the amplified...

  14. DNA Purification Without a Kit

    Type
    Blog Post
    ... milk going to help me extract DNA from agarose gel?!”   The DNA gel extraction with glass milk method... a similar workflow as a column-based plasmid extraction kit. First, you lyse the bacteria and denature...contamination and you can use a phenol chloroform extraction to remove remaining proteins and RNase. Finally...ready for use! Plasmid purification and DNA gel extraction with glass syringe filters Starting material:...fragments The plasmid purification and DNA gel extraction method described in Kim and Morrison is nearly...protocol and guanidine isothiocyanate for the gel extraction protocol) disrupts the DNA’s hydrogen bonds with... more flexible than commercial kits.  DNA gel extraction with glass milk Starting material: agarose gel...

  15. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...information from a region (anterograde tracer, Fig. 1B). Tracers are typically delivered to a particular... either trace downstream from the soma (neuron cell body) toward the axon (anterograde tracer) or upstream...making them useful tracers, but finding a virus that works well for retrograde tracing can be difficult....brain works. There are two methods of neuronal tracing (Fig. 1): one to visualize neurons that send information...information to a region (retrograde tracer, Fig. 1A), and another to visualize neurons that receive information...from the axon terminals to the soma (retrograde tracer) (Fig. 2). Viruses as neuronal scalpels Viruses...transport and can therefore perform retrograde tracing, but often at the cost of toxicity, low expression...

  16. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...days. The crRNA and tracrRNA are annealed on a PCR block and the annealed cr:tracrRNA is then mixed with...Anneal crRNA and tracrRNA using a PCR block. Add Cas9 protein to the annealed cr:tracrRNA and incubate at...Tag 96 genes at a time using Custom Synthetic cr:tracrRNA/Cas9 Ribonucleoprotein (csRNP) delivery Epitope...started off by using custom synthetic two-part cr/tracrRNAs for CRISPR experiments (in collaboration with ...-tagging experiments. Cas9 protein, annealed cr:tracrRNA, and single-stranded donor DNA. The success of...have recently demonstrated that the two-part cr:tracrRNA system is much more potent than the in vitro-...Dewari et al. 2018 and Figure 2). Not only is the cr:tracrRNA approach highly efficient, it is also straightforward...

  17. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ... challenging to track intracellularly, but aptamers permit the study of intracellular RNA dynamics. Aptamers...its ability to track RNA dynamics in a model of Fragile-X tremor/ataxia syndrome (Strack et al., 2013)....target cellular RNA to specific intracellular locations using Cas9 and track the localization of the complex... molecule. Fluorescent RNA aptamers are used to track cellular processes by transforming cells with plasmids...then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal cells (Nilaratanakul...is better able to maintain its fluorescence intracellularly due to a lower dependency on magnesium and ...bright, high-affinity complex for RNA labeling and tracking." ACS chemical biology 9.10 (2014): 2412-2420....

  18. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...video abstracts identified. The journal Cell had the largest average view count per video abstract at 2,947...antibiotic prescriptions for respiratory tract infections. The video abstracts provide a brief introduction to ...science communication through video     Video abstracts about research, especially published research,... views) rounded out the top four average video abstract view counts.” For researchers looking to garner...garner more attention for their work, a video abstract may be a useful tool for turning video views into ...there was a growing number of online research abstracts. With journals in medicine, physics, math, and...), I created a new platform for sharing video abstracts about research: www.WeShareScience.org. WeShareScience...

  19. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...of common genetically encoded neuronal tracers Neuronal tracers such as epitope tags and fluorescent proteins...molecules in your favorite neuronal population and track connections to that population. Limitations of ... used for protein localization, isolation, and tracking experiments (Rizzo, 2009). With many different...Spaghetti Monster - A new and improved neuronal tracer With the limitations of epitope tags and fluorescent...fluorescent proteins, it has been crucial to develop new tracers that enable accurate labeling of neurons. As discussed...expressed proteins in particular are difficult to track with these classic probes. To overcome the limitations...Addgene Uses of smFPs 1. Multi-channel connectomic tracers Fluorescent proteins such as GFP have been used...

  20. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...called Simultaneous Tracking of Recombinase Activation and Clonal Kinetics (STRACK) to study stem cell...several viral tools can perform neuronal tracing, anterograde tracing poses challenges, from non-selective...While many barcoding libraries are available for tracking cell lineage, different applications require careful...pre-existing heterogeneity of hematopoietic stem cells and track clonal dynamics during differentiation after they...labeling, making it a powerful tool for circuit tracing and other neuroscience applications (Figure 4).... single synapses, a key attribute for neuronal tracing. Labeling was nontoxic and activity-dependent, ...itself could be expressed Cre-dependently to allow tracing exclusively from genetically determined cells. ...
Showing: 61 - 80 of 506 results