We narrowed to 105 results for: UBC

1. Viral Vectors 101: Chemogenetics

   Type

   Blog Post

   ... where specific tissues, cell types, or even subcellular regions of a neuron need to be targeted. Targeting...

2. Tips for Titering Your Lentiviral Preps

   Type

   Blog Post

   ...sorting based on expression of a surface marker, subcloning, expansion, and confirmation. This procedure ...

3. Viral Vectors 101: The Retroviral Lifecycle

   Type

   Blog Post

   ...been developed into retroviral vectors and 2.) a subclass of retroviruses called the lentiviruses (like ...

4. Negative Can Be Positive: Open AAV Data with Addgene

   Type

   Blog Post

   ...and Share AAV Data with Addgene's New AAV Data HubCelebrate Open Data Day with Addgene’s AAV Data Hub!Data...

5. Neuronal labeling with Spaghetti Monster

   Type

   Blog Post

   ...structures GFP is limited in its ability to label subcellular structures in the CNS. GFP and other FPs have...

6. Save Time with Transient Plant Leaf Transformations

   Type

   Blog Post

   ...fused to a protein of interest to elucidate the subcellular localization of your protein of interest. Find...

7. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

   Type

   Blog Post

   ... in vitro without antibiotic selection. In a subcutaneous cancer model, the researchers showed that after...

8. Hot Plasmids - November 2023

   Type

   Blog Post

   ...Andrews, N.P., et al. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

9. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   ...transductions. Cell lines should be routinely subcultured at densities that are low enough to prevent confluency...

10. Tips for Using FRET in Your Experiments

    Type

    Blog Post

    ... Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian...

11. Hot Biosensors 2022: Year-End Roundup

    Type

    Blog Post

    ...temperature variations and heat transfer at the subcellular level requires a probe with fast kinetics and...

12. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type

    Blog Post

    ...bait protein can even target BirA* to specific subcellular locations, such as the nuclear envelope, allowing...

13. Plasmids 101: Common Lab E. coli Strains

    Type

    Blog Post

    ...only includes general lab strains designed for subcloning or protein expression. If you were to include...

14. Technologies Enabled by NanoLuc® Luciferase

    Type

    Blog Post

    ...multiple cellular events such as the dynamics of subcellular structures and gene expression. You must see ...

15. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    ... as seen with other epigenome-editing tools. pLV hUbC-dCas9-MQ1(Q147L)-EGFP is also available for lentiviral...

16. Antibodies 101: The Basics of Western Blotting

    Type

    Blog Post

    ...immunoprecipitation and are a common readout for subcellular fractionations, a procedure that isolates proteins...

17. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    ... J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

18. Antibodies 101: Validation

    Type

    Blog Post

    ...antibody that you want to use to look at the subcellular localization of a protein of interest. Conveniently...

19. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    ...Fluorescent Protein Guide Pages Find Plasmids for Subcellular Localization Find Fluorescent Protein Empty Vectors...

20. Antibodies 101: Introduction to Antibodies

    Type

    Blog Post

    ...antigen to myeloma cells. The hybridomas are then subcloned by limiting dilution, which produces an immortalized...