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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 577 results for: Vit

Showing: 61 - 80 of 577 results

  2. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...CRISPR systems with wiki entries describing enzyme activity, experimental considerations, and more, created...

  4. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...Comparing SpCas9 and GeoCas9. *For in vitro Cas9 RNP activity or longevity. GeoCas9 is active at high temperatures...Optimal Activity 35 - 45 °C 50 - 70 °C Genome Editing in Mammalian Cells? Yes Yes Longevity in Serum... from 25-80 °C. Activity is expressed as Kcleave (min-1). SpCas9 has maximum activity from 35-45 °C, while... while GeoCas9 has maximum activity from 50-70 °C. The key advantage of GeoCas9 is that it functions... be incubated at high temps but still maintain activity following heat treatment. The second set of experiments...Harrington et al for more examples of GeoCas9’s editing activity in HEK293’s. GeoCas9 stability in human plasma...trick GeoCas9 has up its sleeve is its increased longevity in human serum. Harrington et al suspected GeoCas9...

  7. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...NGG) 51306 pUC57-Simple-gRNA backbone T7 BsaI In vitro transcription none, need Cas9 plasmid Chen Do you...

  8. AAV Viral Preps

    Type
    Collection
    ...is used in a wide range of experiments, both in vitro and in vivo. It has low immunogenicity, is easy ...

  12. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ... a unique enhancer and either Ascl1 or Neurog2 activity . Development, 148(12). doi: 10.1242/dev.199399...

  13. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...sequences, Cas12a activity gets activated. This unleashes indiscriminate ssDNA cleavage activity which cleaves...Specific ​H​igh ​s​ensitivity ​E​nzymatic ​R​eporter un​LOCK​ing) technique Sensitivity: Single molecule...Two-step-SHERLOCK has a sensitivity in the zeptomolar range while One-step-SHERLOCK has a sensitivity in the femto-aptomolar...SARS-CoV-2 using a CRISPR based DETECTR assay Sensitivity:10-100 copies of RNA per microliter Time: ~40... need for thermocyclers. This is followed by in vitro transcription and Cas13 is then used to detect the...visual detection of novel coronavirus SARS-CoV-2 Sensitivity: 4.6-11 copies/microliter of RNA targets in a...two crRNAs that bind to two regions improves sensitivity of the assay. This reaction also contains SSB...

  14. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...switch and turn off CRISPR activity. The ability to turn off CRISPR activity is an important step towards...thing that was missing: a way to shut off Cas’s activity. The concern is that the longer Cas remains active...edits to occur. Although methods to switch on Cas activity using light or drugs have been developed, the ... the labs found that anti-CRISPR prevents Cas9 activity in HEK293 by reducing editing from ~30% in control...proteins are highly diverse but most block CRISPR activity one of three ways: Inhibit DNA binding Example... Anti-CRISPR proteins (Acr's) can block CRISPR activity in many ways such as inhibiting crRNA loading,...experiments 1. Reduce off-target effects Prolonged Cas activity can increase the chance of off-target editing ...

  15. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...CRISPR systems with wiki entries describing enzyme activity, experimental considerations, and more, created...

  16. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...rapidly detect nucleic acids with high selectivity and sensitivity from patient samples. Find the DETECTR...nucleic acid detection however, either lack the sensitivity or the specificity to detect nucleic acids at...level. The Zhang lab has adapted natural RNase activity of the Cas13 protein to develop and optimize the... the method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2) (Gootenberg...CRISPR-based diagnostics. SHERLOCK- Specific High Sensitivity Enzymatic Reporter UnLOCKING Cas13 (C2c2) was... your targeted nucleic acid. To increase the sensitivity of the assay, targeted DNA or RNA from a sample... SHERLOCK to detect DNA or RNA with atomolar sensitivity and single base pair mismatch specificity.  SHERLOCKv2...

  17. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...to control Rho GTPase activity. A) Schematic of Opto-RhoGEF design and activity. B) OptoTIAM (magenta)... light-sensitive ion channels used to control activities like neuronal signaling — think of a mouse with...there’s a lot more to optogenetics than neuronal activity. Researchers have designed a variety of photoswitchable...severing. A) Schematic of Opto-katanin constructs and activity. EB3N-VVDfast-iLID (anchor) and SspB-mCherry-p60...microtubule severing and has very low dark-state activity and toxicity. All of that means this light-powered...called SuperPLDs with 10, 30, 50, and 100 times the activity of the original, generated using directed evolution... with high efficiency in mammalian cells and in vitro, opening up a wide realm of possibilities. The CRY2...

  18. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...for its role in molecular biology, biotin (Vitamin H or Vitamin B7) is first an essential coenzyme that ...determined to be due to a deficiency in Vitamin H later renamed Vitamin B7/biotin. Egg white injury would plague...biotinylation. This 13 amino acid sequence is termed AviTag. Using AviTag, one can label a protein of interest with...bacterial fusion proteins Biotin is an essential vitamin for life Although biotin is most commonly known...bacteria and some fungi, thus mammals must obtain the vitamin through external sources. Humans absorb most of... biotinylate macromolecules of interest both in vitro and in vivo. Biotinylation reagents Biotin’s exposed...Scientific's Biotinylation Reagent Selection Tool. AviTag and BirA Proteins can also be biotinylated by exploiting...

  19. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...can interfere with activity. The resulting system was coined as CRISPR–Cas9 activity switching via a novel...stemmed from the goal of reducing off-target Cas9 activity. The use of nickases, prime editing, anti-CRISPR...targeting specificity or reduce the duration of Cas9 activity.  The field of optogenetics is renowned for enabling...such as microbial opsins, to control cellular activities using light. In 2015, scientists combined CRISPR...NLSx3-CRYPHR-p65 - it had the lowest background activity in the dark state and highest fold induction at...where light is required to reconstitute enzymatic activity. Photoactivatable genome modifications by NHEJ...low background and high fold-induction of Cas9 activity (16.4-fold). This paCas9-1 light-inducible system...

  20. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ... chance of cross-reactivity Higher level of background stainingLess sensitivityLess specificityLimited...specificityIncreased chance of cross-reactivity Sandwich High specificityHigh sensitivity Longer protocolIncreased...moleculesReduced chance of cross-reactivity Less sensitivityLess specificityLimited availability of conjugated... ELISAs for their accuracy, specificity, and sensitivity (a good ELISA can detect picogram quantities ...procedure removes one potential source of cross-reactivity.   While the method is simple and saves time,...Incorporating a secondary antibody increases the sensitivity of the assay, since multiple secondary antibodies...secondary antibodies can also be a source of cross-reactivity.  Sandwich ELISAs In the most common type of...
Showing: 61 - 80 of 577 results