...temperature. Individual plasmids can be ordered from each plasmid page and will be shipped as bacterial stabs...

...a tool in mammalian cell systems, silencing is achieved through the delivery of a double-stranded RNA ...

...The Framework for In vivo T cell Screens (FITS) approach (Milling and Markson et al., 2024), developed ...compatible with single gene KO and pooled screening approaches; (4) encodes a Thy1.1 selectable marker to facilitate... et al., 2024.   Proof of concept The FITS approach was used to optimize conditions for a screen that...

...thanks to our guest bloggers Joachim Goedhard and Marieke Mastop! Joachim Goedhart is an assistant professor...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...fluorescent probes. You can follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the...

...specific cells, but there are too few resources to teach students how to perform these experiments in the...different genes cloned into plasmids to investigate for each of these planarian species. These plasmids all have... through physical blocking of the translation machinery. In the Sánchez lab protocol, dsRNAs are delivered...Alejandro Sánchez Alvarado, The American Biology Teacher, Vol. 79 No. 3, March 2017; (pp. 208-223). Link...

...explore alternate approaches. Being a “lazy” geneticist, I developed a co-selection approach relying on repair...with unprecedented ease. These new C. elegans approaches rapidly enrich for editing events without the...phenotypes (unc-119) (Dickinson et al., 2013). These approaches allowed effective recovery of knock-ins, but ...several manuscripts published in Genetics detailed approaches in which selection for an editing event that ...and knock-ins at other genomic loci. The first approach – co-CRISPR from Craig Mello’s lab – used inactivation...animals with particular visible phenotypes. These approaches may become even more powerful following a report...Paix et al., 2014). A further development of this approach reported in 2017 uses the direct injection of ...

... can a coach do that is different from an adviser, boss or mentor? First, professional coaches have experience...of the benefits of coaching relationships and do not know where to look for a coach. Many (most?) graduate.... However most coaches who focus on scientists are sensitive to this and have coaching plans that are ...find a coach? Start with referrals. Are there scientists you know who have had a successful coaching experience... assess if the coach you are interviewing is the one for the job. A professional coach will provide an...their track record? Look for a coach that specializes in scientist coaching. Always ask for references...to the coach about their “style” and make sure this is a style that will help you. A good coach usually...

...Resources iGEM - International Genetically Engineered Machine Foundation Registry of Standard Biological Parts...

...including the specific prefix and suffix sequences for each (these can be built into PCR primers when creating...

...and OZ544 OPEN OPEN aCCTCGCCTCagtgtGACGGAGGAc Swachmann-Bodian-Diamond Syndrome Gene (sbds) OZ545 and ...

...generate due to expense and time. An alternative approach is to use synthetically produced recombinant antibodies...

...virus ORF library : Expression library that contains each of the predicted open reading frames for vaccinia...

...stark contrast to Cas9 and Cpf1, which require that each DNA target have high sequence identity to the spacer...

...conducted by European Outreach Scientist Benoit Giquel. Ben recently spoke with Joachim Goεdhart, a professor...the interview with Joachim Goedhart Podcast breakdown Intro Chapter 1 - How Joachim ended up at the University...art. Outro Many thanks to our guest, Joachim Goεdhart! Joachim Goedhart is an assistant professor at...University of Amsterdam Chapter 2 - Joachim’s Academic and career goals. Career advice for people going... microscopy. Chpater 4 - Research projects in Joachim’s lab. New microscopy probes and more. Chapter 5...fluorescent probes. You can follow him on twitter: @joachimgoedhart.   Special thanks to our sound engineer/producer...Fluorescent Proteins featured topic page Read Joachim's Series on Choosing the B(right)est Fluorescent...

...single-cell) approach is to interrogate state with qPCR. The re-arrangement of DNA segments in each state of... our paper, “Synthetic recombinase-based state machines in living cells” (1), into a more straight-forward...our engineered devices, recombinase-based state machines for your own experimental needs. For more context...the paper. Why might one be interested in state machine technology? Biological research has produced a ...timing of these factors. Recombinase-based state machines (RSMs), which take on a particular DNA-sequence... prescribed region of DNA termed the “register”. Each chemical input to an RSM drives expression of a ... set of possible downstream states that can be achieved by expression of other recombinases. Multiple ...

...replication of RNA in the absence of evolved enzymatic machinery (i.e. RNA replication with no proteins). The chemistry...

...new possibilities for GFP-labeled lines, and they each have their own advantages. T-DOGGs can be made drug-inducible...

...of patients results in billions of dollars spent each year. Unfortunately, the causes of most neurodegenerative...

...tagging in your system, use the plasmids as listed in each row. If your own gene of interest is currently unavailable...