We narrowed to 84 results for: apoe

1. March for Science

   Type

   Blog Post

   ...general (1). Science and politics? Science is an apolitical process for seeking knowledge. The process begins...

2. Quick Guide to Near-Infrared Fluorescent Proteins

   Type

   Blog Post

   ...depends on the efficiency of BV incorporation by an apoprotein and protein expression level. State-of-the-art...

3. Viral Vectors 101: Optogenetic Tools

   Type

   Blog Post

   ...considering for your experiment, note that you cannot extrapolate results from one cell type to another, as they...

4. A History of Genome Engineering in Popular Culture

   Type

   Blog Post

   ...what would happen if these corporations would extrapolate genetic engineering to exploit human beings. ...

5. Cancer and the Immune System: Deciphering the Relationship

   Type

   Blog Post

   ...them are a few methodologies being researched. Apoptotic and necroptotic tumor cells have also been tested...

6. Antibodies 101: Flow Cytometry

   Type

   Blog Post

   ...permeabilization with detergents such as triton-x, saponin, or tween. Take care when using strong detergents...

7. Water Bath Protocol

   Type

   Protocol

   ...on the surfaces of the water bath as the water evaporates from the tub. Disinfectant may be added to prevent...cover on the top of the water bath to prevent evaporation and maintain the desired temperature. This also...

8. Deep Dive: qPCR

   Type

   Blog Post

   ...configurations can help you identify pipetting or evaporation errors. And if time allows, you can fit in a ...

9. Antibody Validation Using the Indirect ELISA Method

   Type

   Protocol

   ...well ventilated area and avoid breathing in the vapors. Workflow Timeline Day 1: Antigen Coating Day 2...Desmin (blue) or a negative control protein, Apolipoprotein L3 (red) overnight, blocked, and incubated ...

10. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type

    Blog Post

    ...Chen et al., 2018). Using the cytidine deaminase, APOBEC1 and a nickase version of Cas9 they were effectively...

11. CRISPR Plasmids - Base Edit

    Type

    Collection

    ...dead” Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors are targeted to a specific locus ...

12. mTOR Pathway

    Type

    Collection

    ...the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears to signal upstream...

13. 22 Hot Plasmid Technologies from 2014

    Type

    Blog Post

    ...AAV expression vectors for certain variants. Klapoetke et al., Nat Methods 2014 Mar 11;(3):338-46. ...

14. CRISPR Pooled gRNA Libraries

    Type

    Collection

    ...tuberculosis M. smegmatis Green monkey ( C. sabaeus ) Kaposi's Sarcoma-associated Herpes Virus (KSHV) Cow ( B...

15. 27 Hot Plasmids from 2016

    Type

    Blog Post

    ...processes such as differentiation, proliferation, and apoptosis. In humans, mutations that permanently activate...

16. Validated gRNA Sequences

    Type

    Collection

    ...GAAGCGGGCAAAGGGGCGAC 58780 cut/nick S. pyogenes 24954249 Yamamoto apoea D. rerio GGATGAGCCAAGAAGCCGCT 42241 cut S. pyogenes...

17. Trimmer Lab NeuroMab Collection

    Type

    Collection

    ...IgG2a 188214 Anti-GST [N100/13R ] GST Schistosoma japonicum Mouse IgG2a 188215 Anti-TrpC7 [N64A/36R ] TrpC7...GST scFv [N100/13] N100/13 scFv GST Schistosoma japonicum Mouse 190522 Aldh1L1 scFv [N103/31] N103/31 scFv...

18. Protocol - How to Run an Agarose Gel

    Type

    Protocol

    ...overboil the solution, as some of the buffer will evaporate and thus alter the final percentage of agarose...

19. AAV Titration by qPCR Using SYBR Green Technology

    Type

    Protocol

    ...of causes, such as pipetting error and sample evaporation. Make the master mix after all the samples have...

20. Western Blot

    Type

    Protocol

    ...BSA standard concentration versus absorbance. Extrapolate the total protein concentration of the sample...